YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: SPO14

General Information:
Name(s) found: SPO14 / YKR031C [SGD]
Description(s) found:
Found 22 descriptions.
SHOW ALL
  • Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1683 amino acids

    Gene Ontology:
    Cellular Component: endosome [IDALink to PubMed article]
    prospore membrane [IDALink to PubMed article]
    Biological Process: ascospore-type prospore formation [IMPLink to PubMed article]
    phospholipid metabolic process [IDALink to PubMed article][IMPLink to PubMed article]
    exocytosis [IGILink to PubMed articleLink to PubMed article]
    Molecular Function: phosphatidylinositol-3-phosphate binding [IDALink to PubMed article]
    phospholipase D activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNVSTASGT HFAPPQADRS VTEEVDRVNS RPDELENQEV LRQLPENGNL TSSLQREKRR  60
   61 TPNGKEAERK HALPKSFVDR NLSDVSPNHS LDHIMHSNEH DPRRGSDEEN MHRLYNNLHS 120
  121 SNNNVHSKRN SKREEERAPQ RRSSSVAYTQ QQFNGWKKEF GHAFKKISAI GRLKSSVNSP 180
  181 TPAGSGHRHN QHQHQQVNEE DLYTQRLASD LLDSLLAGCP ASLFASTQFL RDEHGKRRAP 240
  241 LLLAKLDVRV SPLKNDNNIL DITNSNHNHR GNNNNNTGEN SDRRPSIPRS SSIISISSNV 300
  301 AEFMYSRNEN SLFRIHLEYG IDEDRLKWSI IRSYKDIKSL HHKLKIVAFQ QLTISKLYSD 360
  361 NNRYHSLQLP HFPHYKEMVK ERNVMEKKAE NKPSSAASAP HTSENNNNDN GSNITSLETL 420
  421 SSSEISEFNI DNVKMKHLQD LIDEPDDFSQ PIHLRLERYL RLLNIALCLR PHANRLFEFY 480
  481 ELSPLGNLLS RESGFQGKQG YLVIRSTAKA QGWRVSHFGK HAFKDMIDRH TTKWFLVRNS 540
  541 YLTYVSDLSS TTPLDVFLID WKFKVRFSGN KNNILDNENE INWIIHDPNL EINDELEEFG 600
  601 IENDANNILD KNGKSKTHQK KSNISSKLLL LTLENSERKL KIICKSESSL KQWMSSIIKM 660
  661 STSTPWSKPN RFGSFAPVRT NSFCKFLVDG RDYFWSLSEA LLMAKDVIYI HDWWLSPELY 720
  721 LRRPVKGNQG FRIDRMLKSC AEKGIKIFIV IYRNVGNIVG TDSLWTKHSM LNLHPNIHII 780
  781 RSPNQWLQNT YFWAHHEKFV VIDETFAFIG GTDLCYGRYD TFEHVLRDDA ESLLDQNFPG 840
  841 KDYSNARIAD FHDLDKPFES MYDRKVIPRM PWHDVQMMTL GEPARDLARH FVQRWNYLLR 900
  901 AKRPSRLTPL LTPPSDLTAE ELKSLPMFEI LREKSTCETQ ILRSAGNWSL GLKETECSIQ 960
  961 NAYLKLIEQS EHFIYIENQF FITSTVWNGT CVLNKIGDAL VDRIVKANQE KKPWKAFILI1020
 1021 PLMPGFDSPV DTAEASSLRL IMQFQYQSIS RGEHSTFSKL KKLNIDPAQY IQFFSLRKWS1080
 1081 TFAPNERLIT EQLYVHAKIL IADDRRCIIG SANINERSQL GNRDSEVAIL IRDTDLIKTK1140
 1141 MNGDDYYAGK FPWELRQRLM REHLGCDVDL VEFVEKKFER FEKFAAKNYE KLHTLSKEGD1200
 1201 SGNNWSDREM IDSAMIELGY REIFGCKFSP QWKSGHGNSV DDGSTQCGIN EKEVGREDEN1260
 1261 VYEKFFNSVD YGKSSRKRTP LPKHNFASLG LTFNHRAGIE NVGIRDHKVL STDPRLRKND1320
 1321 EHKKEVDGYG PDCWKKESNK KFKADATEQL KEWALNSLAS KVLDDKEMIK SEIPEGFSNY1380
 1381 LPNEKDLEMY LTDKTVTNRN KWSMLKRICY LQYLSHKLDE RKTQRLKKIK DMRRHLSSST1440
 1441 ESTRNGSNSL PLNEKSNEGE STNVDQDIEG DEYHRLHEDI LKNQELDDGS LDDLLSQIIP1500
 1501 KITNFNSGEI DDAKKEELLK LNFIDPYSFE DPLISSFSEG LWFTIALRNT LLYKLVFHCQ1560
 1561 PDNAVQNWKE YGEFTELEQE FQINQEKLID LEAENINSTT TNVVDKDREK EKMRKAAELR1620
 1621 MKLSGSLLYG FNQKVFDKHT AQRILERIHG HLVIFPTEWL AKEVESRNWI FNSDRLSPME1680
 1681 IYN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Gavin AC, et al. (2006) SPO14 YGL036W
    View Details Gavin AC, et al. (2002) MUM2 SPO14 YGL036W
    View Details Qiu et al. (2008) GIP1 SPO14

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run PAN1 No Comments Toshima J, et al (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SPO14 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..178] deduced N/A No confident structure predictions are available.
    2 View Details [179..386] MSA 1.012992 View MSA. No confident structure predictions are available.
    3 View Details [387..672] deduced N/A No confident structure predictions are available.
    4 View Details [673..1204] PSI-BLAST 24.69897 Phospholipase D
    5 View Details [1205..1683] deduced N/A No confident structure predictions are available.
    6 View Details [1227..1511] deduced N/A No confident structure predictions are available.
    7 View Details [1512..1683] MSA 1.010978 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • binding
    		•  2.35228805972571 bayes_pls_golite062009
  • protein binding
    		•  1.69714467506208 bayes_pls_golite062009
  • phosphoinositide binding
    		•  1.68899406133945 bayes_pls_golite062009
  • phospholipid binding
    		•  1.58244376007826 bayes_pls_golite062009
  • lipid binding
    		•  0.431109628784594 bayes_pls_golite062009
  • catalytic activity
    		•  0.144334987824229 bayes_pls_golite062009
  • phosphatidylinositol-3-phosphate binding
    		•  0.0470750400040636 bayes_pls_golite062009
    3 No functions predicted.
    4
    Term Confidence Notes
  • phosphoric diester hydrolase activity
    		•  1.55477881010087 bayes_pls_golite062009
  • catalytic activity
    		•  1.29236214516383 bayes_pls_golite062009
  • binding
    		•  1.28229897975449 bayes_pls_golite062009
  • phosphoric ester hydrolase activity
    		•  0.590797893558058 bayes_pls_golite062009
  • protein binding
    		•  0.438178121330569 bayes_pls_golite062009
    5 No functions predicted.
    6 No functions predicted.
    7 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.92

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle