Protein Overview: AVT3

General Information:
Name(s) found:	AVT3 / YKL146W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322702\|ref\|NP_012776.1\| Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt3p [Saccharomyces cerevisiae] [NCBI NR] gi\|549723\|sp\|P36062.1\|AVT3_YEAST RecName: Full=Vacuolar amino acid transporter 3 [NCBI NR] pir\|\|S37976 hypothetical protein YKL146w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486252\|emb\|CAA81988.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (P36062) Hypothetical 75.5 kDa protein in SDH1-CIM5/YTA3 intergenic region [Swiss-Prot] Vacuolar amino acid transporter 3 OS=Saccharomyces cerevisiae GN=AVT3 PE=1 SV=1 [Swiss-Prot] AVT3 Protein involved in amino acid efflux from the vacuole [mips] Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters [SGD] YKL146W, Chr XI from 171785-173863 [yeast_orfs2] Chr XI from 171785-173863 [yeastorf3.fasta] Chr XI from 171784-173862 [yeastorf4.fasta] AVT3 SGDID:S0001629, Chr XI from 171788-173866, Verified ORF [yeastflysequest.fasta] Avt3p [Saccharomyces cerevisiae]gi\|486252\|emb\|CAA81988.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|549723\|sp\|P36062\|YKO6_YEAST Hypothetical 75.5 kDa protein in SDH1-CIM5/YTA3 intergenic regiongi\|539210\|pir\|\|S37976 hypothetical protein YKL14 [nrdb_11152004_Chlamydomonas] AVT3 SGDID:S000001629, Chr XI from 171788-173866, Verified ORF, "Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Vacuolar amino acid transporter 3 [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; Avt3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Vacuolar amino acid transporter 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AVT3 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] AVT3 SGDID:S000001629, Chr XI from 171783-173861, Genome Release 64-1-1, Verified ORF, "Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters" [yeast-030211-contam.fasta] AVT3 SGDID:S000001629, Chr XI from 171783-173861, Genome Release 64-2-1, Verified ORF, "Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters" [20141208_orf_trans.fasta] YKL146W SGDID:S000001629, Chr XI from 171783-173861, Genome Release 64-1-1, Verified ORF, "Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	692 amino acids
Gene Ontology:
Cellular Component:	fungal-type vacuole membrane [IMP]
Biological Process:	vacuolar transport [IGI][IMP] amino acid transport [IGI][IMP]
Molecular Function:	L-isoleucine transmembrane transporter activity [IGI][IMP] L-glutamine transmembrane transporter activity [IGI][IMP] transporter activity [ISS] L-tyrosine transmembrane transporter activity [IGI][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNGKEVSSGS GRTQSNNNKK NNNGGSTGIS HASGSPLTDG NGGNSNGNSR SRSRSRKSSG  60
   61 TTGGLLKKPP LLVNNEAVHA SVPDASHTSC NNGTLEVSIN NPEPHVVDAV ARHLIRNPSN 120
  121 SLQLQGGDIT RDLYKWTNDH PSSPSQYQYP SQPALSTSIP SQAPSFSNRK RSMSFSAASI 180
  181 ASSSHLNNNS EANGNPLAAI GLAPAPMTHE EIRAPGGFRR SFIIQKRRKH NVDAPIPNFF 240
  241 TRNFIEFLTL YGHFAGEDLS EEEEEEEETE EEPEEEALET ESTQLVSREH GRHPHKSSTV 300
  301 KAVLLLLKSF VGTGVLFLPK AFHNGGWGFS ALCLLSCALI SYGCFVSLIT TKDKVGVDGY 360
  361 GDMGRILYGP KMKFAILSSI ALSQIGFSAA YTVFTATNLQ VFSENFFHLK PGSISLATYI 420
  421 FAQVLIFVPL SLTRNIAKLS GTALIADLFI LLGLVYVYVY SIYYIAVNGV ASDTMLMFNK 480
  481 ADWSLFIGTA IFTFEGIGLL IPIQESMKHP KHFRPSLSAV MCIVAVIFIS CGLLCYAAFG 540
  541 SDVKTVVLLN FPQDTSYTLT VQLLYALAIL LSTPLQLFPA IRILENWTFP SNASGKYNPK 600
  601 VKWLKNYFRC AIVVLTSILA WVGANDLDKF VSLVGSFACI PLIYIYPPLL HYKASILSGT 660
  661 SRARLLLDLI VIVFGVAVMA YTSWQTIKMW SQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		AGP2 AVT1 AVT3 AVT4 GAP1 TPO1 TPO3 VBA1 VBA2 VMA9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

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	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD6	AVT3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	AVT3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..282]	MSA	2.714997	View MSA. No confident structure predictions are available.
2	View Details	[283..692]	Pfam	145.69897	Transmembrane amino acid transporter protein No confident structure predictions are available.
3	View Details	[293..692]	PSI-BLAST	3.522879	Crystal structure of LEUTAA, a bacterial homolog of Na+/Cl--dependent neurotransmitter transporters

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2