YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: RAM2

General Information:
Name(s) found: RAM2 / YKL019W [SGD]
Description(s) found:
Found 28 descriptions.
SHOW ALL
  • Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 316 amino acids

    Gene Ontology:
    Cellular Component: protein farnesyltransferase complex [IDALink to PubMed article]
    CAAX-protein geranylgeranyltransferase complex [IDALink to PubMed article]
    Biological Process: peptide pheromone maturation [IDALink to PubMed article]
    protein amino acid farnesylation [IDALink to PubMed article]
    protein amino acid geranylgeranylation [IDALink to PubMed article]
    Molecular Function: protein geranylgeranyltransferase activity [IDALink to PubMed article]
    protein farnesyltransferase activity [IDALink to PubMed article]
    CAAX-protein geranylgeranyltransferase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEEYDYSDVK PLPIETDLQD ELCRIMYTED YKRLMGLARA LISLNELSPR ALQLTAEIID  60
   61 VAPAFYTIWN YRFNIVRHMM SESEDTVLYL NKELDWLDEV TLNNPKNYQI WSYRQSLLKL 120
  121 HPSPSFKREL PILKLMIDDD SKNYHVWSYR KWCCLFFSDF QHELAYASDL IETDIYNNSA 180
  181 WTHRMFYWVN AKDVISKVEL ADELQFIMDK IQLVPQNISP WTYLRGFQEL FHDRLQWDSK 240
  241 VVDFATTFIG DVLSLPIGSP EDLPEIESSY ALEFLAYHWG ADPCTRDNAV KAYSLLAIKY 300
  301 DPIRKNLWHH KINNLN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CDC43 RAM1 RAM2
    View Details (MIPS) Mewes HW, et al. (2004) CDC43 RAM1 RAM2
    View Details Krogan NJ, et al. (2006) CDC43 RAM2
    View Details Gavin AC, et al. (2002) GPM1 RAM1 RAM2
    View Details Qiu et al. (2008) BET2 BET4 CDC43 RAM1 RAM2

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RAM2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..124] PSI-BLAST 343.927757 Protein farnesyltransferase alpha-subunit
    2 View Details [125..208] PSI-BLAST 343.927757 Protein farnesyltransferase alpha-subunit
    3 View Details [209..316] PSI-BLAST 343.927757 Protein farnesyltransferase alpha-subunit

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • protein geranylgeranyltransferase activity
    		•  12.6759732522795 bayes_pls_golite062009
  • protein farnesyltransferase activity
    		•  12.1496415778348 bayes_pls_golite062009
  • prenyltransferase activity
    		•  11.9861287863026 bayes_pls_golite062009
  • CAAX-protein geranylgeranyltransferase activity
    		•  10.4593287273566 bayes_pls_golite062009
  • protein prenyltransferase activity
    		•  8.98854711542899 bayes_pls_golite062009
  • Rab geranylgeranyltransferase activity
    		•  6.64922604670914 bayes_pls_golite062009
  • acetylcholine receptor regulator activity
    		•  2.46174453725203 bayes_pls_golite062009
  • transferase activity
    		•  1.86754243570948 bayes_pls_golite062009
  • binding
    		•  1.29714843812125 bayes_pls_golite062009
  • catalytic activity
    		•  0.874200184943744 bayes_pls_golite062009
  • protein binding
    		•  0.478870166958436 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle