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Protein Overview: PHO90

General Information:
Name(s) found: PHO90 / YJL198W [SGD]
Description(s) found:
Found 22 descriptions.
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  • Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 881 amino acids

    Gene Ontology:
    Cellular Component: membrane [ISSLink to PubMed article]
    Biological Process: phosphate transport [IGILink to PubMed article]
    Molecular Function: phosphate transmembrane transporter activity [IGILink to PubMed article][TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRFSHFLKYN AVPEWQNHYM DYSELKNLIY TLQTDELQVG DNEEGFGAGK SSNITDRFKN  60
   61 KFSFKNAKED TSSGMNKDAG IVEETIELRE LPTAQTVAAK PSPFRRMKEK IFYKRRSSSA 120
  121 SSVSSTANEN LQLDTYDTFV GDLTAEKQKV DDFYKRTEAK FYDKFDALVK DLKKIGVIEY 180
  181 DIDDDTLFNE PIASTNDEVP PLDLDDDEDD DEFYDDQSNI EDNTALLHHS QYNIKSQKKS 240
  241 LLKKSIVNLY IDLCQLKSFI ELNRIGFAKI TKKSDKVLHL NTRTELIESE QFFKDTYAFQ 300
  301 AETIELLNSK ISQLVTFYAR ITDRPHNISH SKQELKSYLH DHIVWERSNT WKDMLGLLSQ 360
  361 ADELTPKETE YNANKLVGKL DLEYYRWPLP RPINLKFTSI NNVALPKLFF TKKAYKIYFI 420
  421 ILVTGLLLGI KTFNDAAQHR CMALVECVAF LWASEAIPLH ITAFLVPLLV VLFKVLKTSD 480
  481 GAIMSAASAS SEILAAMWSS TIMILLAGFT LGEVLAQYNI AKVLASWLLA FAGCKPRNVL 540
  541 LMAMCVVFFL SMWISNVAAP VLTYSLLSPL LDAMDADSPF AQALVLGVAL AANIGGMSSP 600
  601 ISSPQNIISM SYLKPYGIGW GQFFAVALPS GILAMLLVWI LLFTTFKMNK TKLEKFKPIK 660
  661 TKFTVKQYYI ITVTVATILL WCVESQIEGA FGSSGQIAII PIVLFFGTGL LSTQDLNAFP 720
  721 WSIVILAMGG IALGKAVSSS GLLSTIAKAL QKKIENDGVF AILCIFGILM LVVGTFVSHT 780
  781 VSAIIIIPLV QEVGDKLGNP KAAPILVFGC ALLSSCGMGL ASSGFPNVTA ISKVDRKGDR 840
  841 YLSVMTFLTR GVPASILAFL CVITLGYGIM ASVVKGNATS A

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Qiu et al. (2008) PHO90 PHO91

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run YNL313C No Comments Hazbun TR, et al. (2003)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run VAM3 sample: hx3 Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run VAM3 hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex Hao Xu, et al. (2010)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data PHO90 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..294] Pfam 82.259637 SPX domain No confident structure predictions are available.
    2 View Details [295..389] deduced N/A Confident ab initio structure predictions are available.
    3 View Details [390..881] Pfam 6.769551 Sodium:sulfate symporter transmembrane region No confident structure predictions are available.
    4 View Details [646..710] deduced N/A No confident structure predictions are available.
    5 View Details [711..881] MSA 7.256995 View MSA. No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
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