Protein Overview: NEM1

General Information:
Name(s) found:	NEM1 / YHR004C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731630\|sp\|P38757.1\|NEM1_YEAST RecName: Full=Nuclear envelope morphology protein 1 [NCBI NR] gi\|6321791\|ref\|NP_011867.1\| Catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme, which regulates nuclear growth by controlling recruitment of Pah1p onto promoters of phospholipid biosynthetic genes; required for normal nuclear envelope morphology and [NCBI NR] pir\|\|S46802 hypothetical protein YHR004c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|51013351\|gb\|AAT92969.1\| YHR004C [Saccharomyces cerevisiae] [NCBI NR] gi\|500822\|gb\|AAB68431.1\| Yhr004cp [Saccharomyces cerevisiae] [NCBI NR] (P38757) Uncharacterized protein YHR004C [Swiss-Prot] Nuclear envelope morphology protein 1 OS=Saccharomyces cerevisiae GN=NEM1 PE=1 SV=1 [Swiss-Prot] NEM1 Protein required for nuclear morphology [mips] Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard [SGD] NEM1, Chr VIII from 111747-113087, reverse complement [yeast_orfs2] Chr VIII from 111747-113087, reverse complement [yeastorf3.fasta] Chr VIII from 113087-111747, reverse complement [yeastorf5.fasta] (P38757) Hypothetical 50.6 kDa protein in RPL14B-GPA1 intergenic region [4932.FASTACCERV.txt] NEM1 SGDID:S0001046, Chr VIII from 113088-111748, reverse complement, Verified ORF [yeastflysequest.fasta] Nem1p [Saccharomyces cerevisiae]gi\|51013351\|gb\|AAT92969.1\| YHR004C [Saccharomyces cerevisiae]gi\|731630\|sp\|P38757\|YHG4_YEAST Hypothetical 50.6 kDa protein in RPL14B-GPA1 intergenic regiongi\|626594\|pir\|\|S46802 hypothetical protein YHR004c - yeast (Saccha [nrdb_11152004_Chlamydomonas] NEM1 SGDID:S000001046, Chr VIII from 113089-111749, reverse complement, Verified ORF, "Protein of the nuclear envelope required for the spherical shape of the nucleus; required for normal sporulation" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NEM1 SGDID:S000001046, Chr VIII from 113089-111749, reverse complement, Verified ORF, "Catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme, which regulates nuclear growth by controlling recruitment of Pah1p onto promoters of phospholipid biosynthetic [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] NEM1 SGDID:S000001046, Chr VIII from 113089-111749, reverse complement, Verified ORF, "Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelop [yeast-200209-contam.fasta] Yhr004cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein of the nuclear envelope required for the spherical shape of the nucleus; required for normal sporulation; Nem1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YHR004C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nuclear envelope morphology protein 1 [nr-271106-contam.fasta] NEM1 SGDID:S000001046, Chr VIII from 113089-111749, reverse complement, Verified ORF, "Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Nuclear envelope morphology protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NEM1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NEM1 SGDID:S000001046, Chr VIII from 113094-111754, Genome Release 64-1-1, reverse complement, Verified ORF, "Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard" [yeast-030211-contam.fasta] NEM1 SGDID:S000001046, Chr VIII from 113094-111754, Genome Release 64-2-1, reverse complement, Verified ORF, "Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard" [20141208_orf_trans.fasta] YHR004C SGDID:S000001046, Chr VIII from 113094-111754, Genome Release 64-1-1, reverse complement, Verified ORF, "Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	446 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] nuclear membrane-endoplasmic reticulum network [IDA] integral to membrane [IDA][ISS]
Biological Process:	ascospore formation [IMP] nucleus organization [IGI][IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV SEVDQQYDHS  60
   61 SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL LFLPYYLIIK PLMSLWFVFT 120
  121 FPLSVIERRV KHTDKRNRGS NASENELPVS SSNINDSSEK TNPKNCNLNT IPEAVEDDLN 180
  181 ASDEIILQRD NVKGSLLRAQ SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL 240
  241 IPKSVLNTQK KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 300
  301 YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC VLRDGVGYIK 360
  361 DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI QVEGWISDPT DTDLLNLLPF 420
  421 LEAMRYSTDV RNILALKHGE KAFNIN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		NEM1 SPO7
View Details	Gavin AC, et al. (2002)		FKS1 GCD6 NAT1 NEM1 SWI3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ERT1	NEM1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NEM1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..178]	deduced	N/A	No confident structure predictions are available.
2	View Details	[179..446]	Pfam	100.180456	NLI interacting factor-like phosphatase No confident structure predictions are available.
3	View Details	[228..446]	PSI-BLAST	59.39794	No description for 2q5eA was found.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2