Protein Overview: DST1

General Information:
Name(s) found:	DST1 / YGL043W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321395\|ref\|NP_011472.1\| General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; Dst1p [Saccharomyces cerevisiae [NCBI NR] gi\|34810565\|pdb\|1PQV\|S Chain S, Rna Polymerase Ii-Tfiis Complex [NCBI NR] pir\|\|A42921 transcription elongation factor S-II - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|218517\|dbj\|BAA02046.1\| transcriptional elongation factor S-II [Saccharomyces cerevisiae] [NCBI NR] gi\|1729915\|sp\|P07273.4\|TFS2_YEAST RecName: Full=Transcription elongation factor S-II; AltName: Full=DNA strand transfer protein alpha; Short=STP-alpha; AltName: Full=DNA strand transferase 1; AltName: Full=Pyrimidine pathway regulatory protein 2 [NCBI NR] gi\|1322528\|emb\|CAA96744.1\| DST1 [Saccharomyces cerevisiae] [NCBI NR] Transcription elongation factor S-II OS=Saccharomyces cerevisiae GN=DST1 PE=1 SV=4 [Swiss-Prot] DST1 TFIIS (transcription elongation factor) [mips] General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites [SGD] DST1, Chr VII from 417478-418407 [yeast_orfs2] Chr VII from 417478-418407 [yeastorf3.fasta] Chr VII from 417485-418414 [yeastorf4.fasta] (P07273) Transcription elongation factor S-II (DNA strand transfer protein alpha) (STP-alpha) (DNA strand transferase 1) (Pyrimidine pathway regulatory protein 2) [4932.FASTACCERV.txt] DST1 SGDID:S0003011, Chr VII from 417485-418414, Verified ORF [yeastflysequest.fasta] DST1 SGDID:S0003011, Chr VII from 417487-418416, Verified ORF [10112005cervSGD.fasta] Dst1p [Saccharomyces cerevisiae]gi\|1322528\|emb\|CAA96744.1\| DST1 [Saccharomyces cerevisiae]gi\|34810565\|pdb\|1PQV\|S Chain S, Rna Polymerase Ii-Tfiis Complexgi\|283281\|pir\|\|A42921 transcription elongation factor S-II - yeast (Saccharomyces cerevisiae)gi\|17 [nrdb_11152004_Chlamydomonas] DST1 SGDID:S000003011, Chr VII from 417487-418416, Verified ORF, "General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] transcriptional elongation factor S-II [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Transcription elongation factor S-II (DNA strand transfer protein alpha) (STP-alpha) (DNA strand transferase 1) (Pyrimidine pathway regulatory protein 2) [nr-271106-contam.fasta] DST1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Chain S, Rna Polymerase Ii-Tfiis Complex [nr-271106-contam.fasta] General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; Dst1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DST1 SGDID:S000003011, Chr VII from 417487-418416, Verified ORF, "General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Transcription elongation factor S-II OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DST1 PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DST1 SGDID:S000003011, Chr VII from 417483-418412, Genome Release 64-1-1, Verified ORF, "General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites" [yeast-030211-contam.fasta] DST1 SGDID:S000003011, Chr VII from 417483-418412, Genome Release 64-2-1, Verified ORF, "General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress" [20141208_orf_trans.fasta] YGL043W SGDID:S000003011, Chr VII from 417483-418412, Genome Release 64-1-1, Verified ORF, "General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_1503\|g.1111 ORF ID=asmbl_1503\|g.1111 ID=asmbl_1503\|m.1111 type:complete len:310 (+) ID=asmbl_1503:32-961(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	309 amino acids
Gene Ontology:
Cellular Component:	nucleoplasm [IPI]
Biological Process:	reciprocal meiotic recombination [IMP] RNA elongation from RNA polymerase II promoter [IDA] mRNA cleavage [IDA][TAS]
Molecular Function:	positive transcription elongation factor activity [IDA ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV NKFKKSTNVE  60
   61 ISKLVKKMIS SWKDAINKNK RSRQAQQHHQ DHAPGNAEDK TTVGESVNGV QQPASSQSDA 120
  121 MKQDKYVSTK PRNSKNDGVD TAIYHHKLRD QVLKALYDVL AKESEHPPQS ILHTAKAIES 180
  181 EMNKVNNCDT NEAAYKARYR IIYSNVISKN NPDLKHKIAN GDITPEFLAT CDAKDLAPAP 240
  241 LKQKIEEIAK QNLYNAQGAT IERSVTDRFT CGKCKEKKVS YYQLQTRSAD EPLTTFCTCE 300
  301 ACGNRWKFS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DST1 IWR1 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC10 RPO21 RPO26 SPT5 TAF14 TFG1 TFG2
View Details	Krogan NJ, et al. (2006)		ALG3 BUD27 CBF2 DST1 ESS1 IWR1 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPO21 RPO26 SPT4 TAF14 TFG1 TFG2 YBL059W YBR259W YPL225W
View Details	Qiu et al. (2008)		CDC73 DST1 ELC1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NTA1	DST1	1	Unpublished Fields Lab Data
View Screen	RAS2	DST1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DST1	Huh WK, et al. (2003)

Protein Structure Data

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Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..103]	PSI-BLAST	330.502279	Transcription elongation factor TFIIS N-domain
2	View Details	[104..190]	PSI-BLAST	574.66807	Elongation factor TFIIS domain 2
3	View Details	[191..258]	ORFEUS	13.27	Elongation factor TFIIS domain 2
4	View Details	[259..309]	PSI-BLAST	155.08661	Transcriptional factor SII, C-terminal domain

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2