Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha

Mol Cell Biol. 1991 May;11(5):2576-82. doi: 10.1128/mcb.11.5.2576-2582.1991.

Abstract

DNA strand transfer protein alpha (STP alpha) from meiotic Saccharomyces cerevisiae cells promotes homologous pairing of DNA without any nucleotide cofactor in the presence of yeast single-stranded DNA binding protein. This gene (DNA strand transferase 1, DST1) encodes a 309-amino-acid protein with a predicted molecular mass of 34,800 Da. The STP alpha protein level is constant in both mitotic and meiotic cells, but during meiosis the polypeptide is activated by an unknown mechanism, resulting in a large increase in its specific activity. A dst1::URA3/dst1::URA3 mutant grows normally in mitotic media; however, meiotic cells exhibit a greatly reduced induction of both DNA strand transfer activity and intragenic recombination between his1 heteroalleles. Spore viability is normal. These results suggest that DST1 is required for much of the observed induction of homologous recombination in S. cerevisiae during meiosis but not for normal sporulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal / genetics*
  • DNA, Fungal / isolation & purification
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Escherichia coli / genetics
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Library
  • Genes, Viral*
  • Genotype
  • Kinetics
  • Meiosis
  • Mitosis
  • Molecular Sequence Data
  • Nucleotidyltransferases / genetics*
  • Nucleotidyltransferases / metabolism
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Viral Structural Proteins / genetics*

Substances

  • DNA, Fungal
  • DNA-Binding Proteins
  • DST1 protein, S cerevisiae
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Viral Structural Proteins
  • Nucleotidyltransferases

Associated data

  • GENBANK/M60416
  • GENBANK/M60770
  • GENBANK/M64820
  • GENBANK/M64821
  • GENBANK/M64822
  • GENBANK/M64823
  • GENBANK/M64824
  • GENBANK/M64825
  • GENBANK/M64826
  • GENBANK/M64827