Protein Overview: GNP1

General Information:
Name(s) found:	GNP1 / YDR508C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|927778\|gb\|AAB64950.1\| Gnp1p: high-affinity glutamine permease; CAI: 0.20 [Saccharomyces cerevisiae] [NCBI NR] gi\|6320717\|ref\|NP_010796.1\| High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; Gnp1p [Saccharomyces ce [NCBI NR] pir\|\|S69566 glutamine transport protein, high affinity - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1708002\|sp\|P48813.2\|GNP1_YEAST RecName: Full=High-affinity glutamine permease [NCBI NR] High-affinity glutamine permease OS=Saccharomyces cerevisiae GN=GNP1 PE=1 SV=2 [Swiss-Prot] GNP1 Broad-specificity amino-acid permease [mips] High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids [SGD] GNP1, Chr IV from 1466456-1468447, reverse complement [yeast_orfs2] Chr IV from 1466456-1468447, reverse complement [yeastorf3.fasta] Chr IV from 1466439-1468430, reverse complement [yeastorf4.fasta] Chr IV from 1468432-1466441, reverse complement [yeastorf5.fasta] (P48813) High-affinity glutamine permease [4932.FASTACCERV.txt] GNP1 SGDID:S0002916, Chr IV from 1468432-1466441, reverse complement, Verified ORF [yeastflysequest.fasta] GNP1 SGDID:S0002916, Chr IV from 1468434-1466443, reverse complement, Verified ORF [10112005cervSGD.fasta] Gnp1p [Saccharomyces cerevisiae]gi\|927778\|gb\|AAB64950.1\| Gnp1p: high-affinity glutamine permease; CAI: 0.20 [Saccharomyces cerevisiae]gi\|1708002\|sp\|P48813\|GNP1_YEAST High-affinity glutamine permeasegi\|2119772\|pir\|\|S69566 glutamine transport protein, hi [nrdb_11152004_Chlamydomonas] GNP1 SGDID:S000002916, Chr IV from 1468434-1466443, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) s [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GNP1 SGDID:S000002916, Chr IV from 1468436-1466445, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) s [yeast-contam.fasta] GNP1 SGDID:S000002916, Chr IV from 1468437-1466446, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) s [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Gnp1p: high-affinity glutamine permease; CAI: 0.20 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] High-affinity glutamine permease [nr-271106-contam.fasta] High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; Gnp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GNP1 SGDID:S000002916, Chr IV from 1468437-1466446, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids" [yeast-bovrabprots.fasta] GNP1 SGDID:S000002916, Chr IV from 1468436-1466445, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids" [yeast-contam_custom.fasta] [chemostatdb.fasta] High-affinity glutamine permease OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GNP1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GNP1 SGDID:S000002916, Chr IV from 1468444-1466453, Genome Release 64-1-1, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids" [yeast-030211-contam.fasta] GNP1 SGDID:S000002916, Chr IV from 1468444-1466453, Genome Release 64-2-1, reverse complement, Verified ORF, "High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YDR508C SGDID:S000002916, Chr IV from 1468444-1466453, Genome Release 64-1-1, reverse complement, Verified ORF, "High-affinity glutamine permease, also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	663 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] plasma membrane [ISS]
Biological Process:	amino acid transport [IDA] proximal/distal pattern formation [IDA]
Molecular Function:	epsilon hydroxylase activity [IGI] amino acid transmembrane transporter activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTLGNRRHGR NNEGSSNMNM NRNDLDDVSH YEMKEIQPKE KQIGSIEPEN EVEYFEKTVE  60
   61 KTIENMEYEG EHHASYLRRF IDSFRRAEGS HANSPDSSNS NGTTPISTKD SSSQLDNELN 120
  121 RKSSYITVDG IKQSPQEQEQ KQENLKKSIK PRHTVMMSLG TGIGTGLLVG NSKVLNNAGP 180
  181 GGLIIGYAIM GSCVYCIIQA CGELAVIYSD LIGGFNTYPL FLVDPALGFS VAWLFCLQWL 240
  241 CVCPLELVTA SMTIKYWTTS VNPDVFVVIF YVLIVVINVF GAKGYAEADF FFNCCKILMI 300
  301 VGFFILAIII DCGGAGTDGY IGSKYWRDPG AFRGDTPIQR FKGVVATFVT AAFAFGMSEQ 360
  361 LAMTASEQSN PRKAIPSAAK KMIYRILFVF LASLTLVGFL VPYTSDQLLG AAGSATKASP 420
  421 YVIAVSSHGV RVVPHFINAV ILLSVLSVAN GAFYTSSRIL MSLAKQGNAP KCFDYIDREG 480
  481 RPAAAMLVSA LFGVIAFCAS SKKEEDVFTW LLAISGLSQL FTWITICLSH IRFRRAMKVQ 540
  541 GRSLGEVGYK SQVGVWGSAY AVLMMVLALI AQFWVAIAPI GGGGKLSAQS FFENYLAMPI 600
  601 WIALYIFYKV WKKDWSLFIP ADKVDLVSHR NIFDEELLKQ EDEEYKERLR NGPYWKRVLD 660
  661 FWC

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GPI14	GNP1	2	Hazbun TR, et al. (2003)
View Screen	ELM1	GNP1	1	Unpublished Fields Lab Data
View Screen	ILM1	GNP1	1	Lockshon D, et al. (2006)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GNP1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..121]	deduced	N/A	No confident structure predictions are available.
2	View Details	[122..663]	Pfam	211.124939	Amino acid permease No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2