Protein Overview: CDC50

General Information:
Name(s) found:	CDC50 / YCR094W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|S19510 probable membrane protein YCR094w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6319937\|ref\|NP_010018.1\| Endosomal protein that regulates cell polarity, controls polarized growth; similar to Ynr048wp and Lem3p; Cdc50p [Saccharomyces cerevisiae] [NCBI NR] gi\|1907232\|emb\|CAA42249.1\| cell division cycle mutant [Saccharomyces cerevisiae] [NCBI NR] gi\|140555\|sp\|P25656.1\|CDC50_YEAST RecName: Full=Cell division control protein 50 [NCBI NR] (P25656) Hypothetical 45.0 kDa protein in NOT1-MATAL2 intergenic region [Swiss-Prot] Cell division control protein 50 OS=Saccharomyces cerevisiae GN=CDC50 PE=1 SV=1 [Swiss-Prot] CDC50 Cell division cycle mutant [mips] Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Ynr048wp and Lem3p [SGD] CDC50, Chr III from 286755-287930 [yeast_orfs2] Chr III from 286755-287930 [yeastorf3.fasta] CDC50 SGDID:S0000690, Chr III from 286758-287933, Verified ORF [yeastflysequest.fasta] Cdc50p [Saccharomyces cerevisiae]gi\|1907232\|emb\|CAA42249.1\| cell division cycle mutant [Saccharomyces cerevisiae]gi\|83268\|pir\|\|S19510 probable membrane protein YCR094w - yeast (Saccharomyces cerevisiae)gi\|140555\|sp\|P25656\|YCY4_YEAST HYPOTHETICAL 45.0 K [nrdb_11152004_Chlamydomonas] CDC50 SGDID:S000000690, Chr III from 286758-287933, Verified ORF, "Endosomal protein that regulates cell polarity; similar to Ynr048wp and Lem3p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CDC50 SGDID:S000000690, Chr III from 286759-287934, Verified ORF, "Endosomal protein that regulates cell polarity; similar to Ynr048wp and Lem3p" [yeast-contam.fasta] CDC50 SGDID:S000000690, Chr III from 286759-287934, Verified ORF, "Endosomal protein that regulates cell polarity, controls polarized growth; similar to Ynr048wp and Lem3p" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] cell division cycle mutant [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Endosomal protein that regulates cell polarity; similar to Ynr048wp and Lem3p; Cdc50p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cell division control protein 50 [nr-271106-contam.fasta] CDC50 SGDID:S000000690, Chr III from 286759-287934, Verified ORF, "Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; s [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] [chemostatdb.fasta] CDC50 SGDID:S000000690, Chr III from 286759-287934, Verified ORF, "Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Ynr048wp and Lem3p" [SGDyeast.20101221.fasta] Cell division control protein 50 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC50 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CDC50 SGDID:S000000690, Chr III from 286762-287937, Genome Release 64-1-1, Verified ORF, "Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Ynr048wp and Lem3p" [yeast-030211-contam.fasta] CDC50 SGDID:S000000690, Chr III from 286762-287937, Genome Release 64-2-1, Verified ORF, "Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YCR094W SGDID:S000000690, Chr III from 286762-287937, Genome Release 64-1-1, Verified ORF, "Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Ynr048wp and Lem3p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	391 amino acids
Gene Ontology:
Cellular Component:	trans-Golgi network [IDA] late endosome [IDA] integral to membrane of membrane fraction [IDA]
Biological Process:	phospholipid translocation [IGI] G1 phase of mitotic cell cycle [IDA][IGI][IMP] endocytosis [IGI] actin cortical patch localization [IGI]
Molecular Function:	phospholipid-translocating ATPase activity [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVSLFKRGKA PPLTKEGPTS KKPPNTAFRQ QRLKAWQPIL SPQSVLPLLI FVACIFTPIG  60
   61 IGLIVSATKV QDLTIDYSHC DTKASTTAFE DIPKKYIKYH FKSKVENKPQ WRLTENENGE 120
  121 QSCELQFEIP NDIKKSIFIY YKITNFYQNH RRYVQSFDTK QILGEPIKKD DLDTSCSPIR 180
  181 SREDKIIYPC GLIANSMFND TFSQVLSGID DTEDYNLTNK HISWSIDRHR FKTTKYNASD 240
  241 IVPPPNWMKK YPDGYTDENL PDIHTWEEFQ VWMRTAAFPK FYKLTLKNES ASLPKGKYQM 300
  301 NIELNYPISL FGGTKSFVLT TNGAIGGRNM SLGVLYLIVA GLCALFGIIF LVKLIFQPRA 360
  361 MGDHTYLNFD DEENEDYEDV HAENTTLREI L

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		CDC50 DRS2 FAT1 PDR16 SEC14 SFH5 STV1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	CDC50 and CHC1	Huh WK, et al. (2003)
View Data	CDC50	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..391]	Pfam	202.187087	LEM3 (ligand-effect modulator 3) family / CDC50 family No confident structure predictions are available.
2	View Details	[135..391]	MSA	4.093995	View MSA. No confident structure predictions are available.

Protein Overview: CDC50

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: