YRC Public Data Repository - Protein Structure Prediction

Protein:	FHLA_ECOLI
Organism:	Escherichia coli
Length:	692 amino acids
Reference:	Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for FHLA_ECOLI.

Description	E-value	Query Range	Subject Range
gi\|115514087, gi... - gi\|117624963\|ref\|YP_853951.1\| formate hydrogenlyase transcriptional activator [Escherichia coli APEC... gi\|218366490, gi... - gi\|218559721\|ref\|YP_002392634.1\| DNA-binding transcriptional activator [Escherichia coli S88], gi\|21...	466.0	[0..1]	[692..1]
gi\|208811906, gi... - gi\|208811906\|ref\|ZP_03253235.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O1... gi\|208821449, gi... - gi\|208821449\|ref\|ZP_03261769.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O1... gi\|209157482, gi... - gi\|209396082\|ref\|YP_002272195.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O... gi\|217327411, gi... - gi\|217327411\|ref\|ZP_03443494.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O1... gi\|189372200, gi... - gi\|189372200\|gb\|EDU90616.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|189377520, gi... - gi\|189377520\|gb\|EDU95936.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|195938483 - gi\|195938483\|ref\|ZP_03083865.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O1... gi\|208805742, gi... - gi\|208805742\|ref\|ZP_03248079.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O1... gi\|13363058, gi\|... - gi\|15832841\|ref\|NP_311614.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:... gi\|189357234, gi... - gi\|189357234\|gb\|EDU75653.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|189366906, gi... - gi\|189366906\|gb\|EDU85322.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|189362115, gi... - gi\|189362115\|gb\|EDU80534.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|168778715, gi... - gi\|189003507\|gb\|EDU72493.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|168749942, gi... - gi\|188015822\|gb\|EDU53944.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:H... gi\|254594072, gi... - gi\|254794672\|ref\|YP_003079509.1\| DNA-binding transcriptional activator [Escherichia coli O157:H7 str... gi\|25308298, gi\|... - pir\|\|C91077 formate hydrogenlyase activator protein [imported] - Escherichia coli (strain O157:H7, s... gi\|15803249, gi\|... - gi\|15803249\|ref\|NP_289281.1\| formate hydrogenlyase transcriptional activator [Escherichia coli O157:... gi\|13363058, gi\|... - gi\|15832841\|ref\|NP_311614.1\| formate hydrogenlyase activator protein [Escherichia coli O157:H7], gi\|...	466.0	[0..1]	[692..1]
gi\|82545196, gi\|... - gi\|82545196\|ref\|YP_409143.1\| formate hydrogen-lyase transcriptional activator for fdhF, hyc and hyp ...	466.0	[0..1]	[692..1]
gi\|110642854, gi... - gi\|110642854\|ref\|YP_670584.1\| formate hydrogenlyase transcriptional activator [Escherichia coli 536]... gi\|190908346, gi... - gi\|191171412\|ref\|ZP_03032961.1\| formate hydrogenlyase transcriptional activator [Escherichia coli F1... gi\|218428419, gi... - gi\|218690855\|ref\|YP_002399067.1\| DNA-binding transcriptional activator [Escherichia coli ED1a], gi\|2...	466.0	[0..1]	[692..1]
gi\|91212091, gi\|... - gi\|91212091\|ref\|YP_542077.1\| formate hydrogenlyase transcriptional activator [Escherichia coli UTI89... gi\|26249130, gi\|... - gi\|26249130\|ref\|NP_755170.1\| formate hydrogenlyase transcriptional activator [Escherichia coli CFT07...	466.0	[0..1]	[692..13]
gi\|110806642, gi... - gi\|110806642\|ref\|YP_690162.1\| formate hydrogen-lyase transcriptional activator for fdhF, hyc and hyp...	462.0	[0..1]	[692..1]
gi\|30042341, gi\|... - gi\|30064085\|ref\|NP_838256.1\| formate hydrogen-lyase transcriptional activator for fdhF, hyc and hyp ... gi\|56480174, gi\|... - gi\|56480174\|ref\|NP_708532.2\| formate hydrogen-lyase transcriptional activator for fdhF, hyc and hyp ...	462.0	[0..1]	[692..1]
gi\|75512359 - gi\|75512359\|ref\|ZP_00734911.1\| COG3604: Transcriptional regulator containing GAF, AAA-type ATPase, a...	457.0	[0..6]	[692..1]
gi\|75256933 - gi\|75256933\|ref\|ZP_00728509.1\| COG3604: Transcriptional regulator containing GAF, AAA-type ATPase, a... gi\|75210932 - gi\|75210932\|ref\|ZP_00711056.1\| COG3604: Transcriptional regulator containing GAF, AAA-type ATPase, a...	457.0	[0..6]	[692..1]
gi\|75235674 - gi\|75235674\|ref\|ZP_00719848.1\| COG3604: Transcriptional regulator containing GAF, AAA-type ATPase, a... gi\|75230063 - gi\|75230063\|ref\|ZP_00716573.1\| COG3604: Transcriptional regulator containing GAF, AAA-type ATPase, a...	457.0	[0..6]	[692..1]

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Predicted Domain #1

Region A:
Residues: [1-219]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSYTPMSDLG QQGLFDITRT LLQQPDLASL CEALSQLVKR SALADNAAIV LWQAQTQRAS  60
   61 YYASREKDTP IKYEDETVLA HGPVRSILSR PDTLHCSYEE FCETWPQLDA GGLYPKFGHY 120
  121 CLMPLAAEGH IFGGCEFIRY DDRPWSEKEF NRLQTFTQIV SVVTEQIQSR VVNNVDYELL 180
  181 CRERDNFRIL VAITNAVLSR LDMDELVSEV AKEIHYYFD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	21.30103
Match:	1mc0A
Description:	3',5'-cyclic nucleotide phosphodiesterase 2A, GAF A and GAF B domains
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
transcription regulator activity	2.80545151006505	bayes_pls_golite062009
DNA binding	2.12853183689157	bayes_pls_golite062009
nucleic acid binding	2.06466906422115	bayes_pls_golite062009
transcription factor activity	2.00805912336419	bayes_pls_golite062009
binding	1.3234252548847	bayes_pls_golite062009
two-component response regulator activity	1.13458435530855	bayes_pls_golite062009
protein histidine kinase activity	1.10591382238785	bayes_pls_golite062009
phosphotransferase activity, nitrogenous group as acceptor	1.05257897401701	bayes_pls_golite062009
catalytic activity	0.319188290441009	bayes_pls_golite062009
two-component sensor activity	0.213200223299938	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [220-628]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 IDDISIVLRS HRKNKLNIYS THYLDKQHPA HEQSEVDEAG TLTERVFKSK EMLLINLHER  60
   61 DDLAPYERML FDTWGNQIQT LCLLPLMSGD TMLGVLKLAQ CEEKVFTTTN LNLLRQIAER 120
  121 VAIAVDNALA YQEIHRLKER LVDENLALTE QLNNVDSEFG EIIGRSEAMY SVLKQVEMVA 180
  181 QSDSTVLILG ETGTGKELIA RAIHNLSGRN NRRMVKMNCA AMPAGLLESD LFGHERGAFT 240
  241 GASAQRIGRF ELADKSSLFL DEVGDMPLEL QPKLLRVLQE QEFERLGSNK IIQTDVRLIA 300
  301 ATNRDLKKMV ADREFRSDLY YRLNVFPIHL PPLRERPEDI PLLAKAFTFK IARRLGRNID 360
  361 SIPAETLRTL SNMEWPGNVR ELENVIERAV LLTRGNVLQL SLPDIVLPE

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	91.39794
Match:	1ny5A
Description:	Crystal structure of sigm54 activator (AAA+ ATPase) in the inactive state
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
protein histidine kinase activity	2.35011582062573	bayes_pls_golite062009
two-component sensor activity	2.32303301074957	bayes_pls_golite062009
phosphotransferase activity, nitrogenous group as acceptor	2.28838869071567	bayes_pls_golite062009
nucleoside-triphosphatase activity	1.95073642969863	bayes_pls_golite062009
pyrophosphatase activity	1.91141407779641	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	1.9017593265179	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	1.90014393273511	bayes_pls_golite062009
carboxylic acid transmembrane transporter activity	1.82370030183942	bayes_pls_golite062009
organic acid transmembrane transporter activity	1.79922369980544	bayes_pls_golite062009
transporter activity	1.55572428045529	bayes_pls_golite062009
amino acid transmembrane transporter activity	1.52217781539459	bayes_pls_golite062009
ATPase activity	1.48474288797022	bayes_pls_golite062009
two-component response regulator activity	1.45919623810582	bayes_pls_golite062009
amine transmembrane transporter activity	1.4031990658196	bayes_pls_golite062009
transmembrane transporter activity	1.38483976298883	bayes_pls_golite062009
ATPase activity, coupled	1.37457738689583	bayes_pls_golite062009
catalytic activity	1.37148167194523	bayes_pls_golite062009
binding	1.30574971150356	bayes_pls_golite062009
active transmembrane transporter activity	1.24278046928678	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	1.2014318795788	bayes_pls_golite062009
primary active transmembrane transporter activity	1.19670408144267	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	1.17063957238561	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	1.16918853509059	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	1.16885789920943	bayes_pls_golite062009
hydrolase activity	1.09839981963412	bayes_pls_golite062009
nucleic acid binding	0.900561861643259	bayes_pls_golite062009
transcription regulator activity	0.530058973650498	bayes_pls_golite062009
purine ribonucleotide binding	0.5127850474754	bayes_pls_golite062009
ribonucleotide binding	0.51230334374838	bayes_pls_golite062009
purine nucleotide binding	0.50688037036449	bayes_pls_golite062009
nucleotide binding	0.49473184426584	bayes_pls_golite062009
DNA binding	0.460315660888175	bayes_pls_golite062009
branched-chain aliphatic amino acid transmembrane transporter activity	0.32754685224555	bayes_pls_golite062009
amine-transporting ATPase activity	0.128277449580549	bayes_pls_golite062009
amino acid-transporting ATPase activity	0.109938795198381	bayes_pls_golite062009
substrate-specific transporter activity	0.0838665746103899	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [629-692]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PETPPAATVV ALEGEDEYQL IVRVLKETNG VVAGPKGAAQ RLGLKRTTLL SRMKRLGIDK  60
   61 SALI

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	89.69897
Match:	1ojlA
Description:	Crystal structure of a sigma54-activator suggests the mechanism for the conformational switch necessary for sigma54 binding
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions: