YRC Public Data Repository - Protein Structure Prediction

Protein:	HRT1
Organism:	Saccharomyces cerevisiae
Length:	121 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

[What does the above image mean?]

[Show Ginzu Version Information]

Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for HRT1.

Description	E-value	Query Range	Subject Range
gi\|4240031 - gi\|4240031\|dbj\|BAA74802.1\| DNA binding zinc finger protein (Pspzf) [Pisum sativum]	1.0E-23	[3..117]	[364..467]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	2.0E-23	[3..121]	[280..388]
RBX1_YEAST - RING-box protein HRT1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HRT1 PE=1 SV=1 HRT1 - RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gi... gi\|151945502 - gi\|151945502\|gb\|EDN63743.1\| Skp1-Cullin-F-box ubiquitin protein ligase (SCF) subunit [Saccharomyces ... gi\|190407219 - gi\|190407219\|gb\|EDV10486.1\| Skp1-Cullin-F-box ubiquitin protein ligase subunit [Saccharomyces cerevi...	6.0E-23	[1..121]	[1..121]
MBR1_ARATH - E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1	3.0E-22	[3..113]	[596..699]
gi\|218187559 - gi\|218187559\|gb\|EEC69986.1\| hypothetical protein OsI_00495 [Oryza sativa Indica Group] gi\|50948809, gi\|... - gi\|9757680\|dbj\|BAB08199.1\| unnamed protein product [Oryza sativa Japonica Group], gi\|8570055\|dbj\|BAA...	3.0E-21	[3..112]	[385..485]
gi\|51980558 - gb\|AAH82183.1\| Unknown (protein for MGC:98645) [Xenopus laevis] gi\|90075658, gi\|... - gi\|90075658\|dbj\|BAE87509.1\| unnamed protein product [Macaca fascicularis], gi\|84579139\|dbj\|BAE73003.... gi\|118082672 - gi\|118082672\|ref\|XP_416239.2\| PREDICTED: similar to RBX1 protein [Gallus gallus] CCDS14009.1_1 - null gi\|114686557 - gi\|114686557\|ref\|XP_515153.2\| PREDICTED: similar to RBX1 protein isoform 5 [Pan troglodytes] RBX1 - ring-box 1 gi\|126338707 - gi\|126338707\|ref\|XP_001363807.1\| PREDICTED: similar to RBX1 protein [Monodelphis domestica] RBX1_MOUSE - E3 ubiquitin-protein ligase RBX1 OS=Mus musculus GN=Rbx1 PE=1 SV=1 gi\|76617022, gi\|... - gi\|88682859\|gb\|AAI05330.1\| Ring-box 1 [Bos taurus], gi\|76617022\|ref\|XP_597082.2\| PREDICTED: similar ... gi\|109451460, gi... - gi\|109452056\|emb\|CAK54890.1\| RBX1 [synthetic construct], gi\|109451460\|emb\|CAK54591.1\| RBX1 [syntheti... HGNC:9928\|MIM:60... - 1 gi\|72679391, gi\|... - gi\|77628004\|ref\|NP_001029307.1\| ring-box 1 [Rattus norvegicus], gi\|72679391\|gb\|AAI00259.1\| Ring-box ... tr\|M3X867\|M3X867... - Ring-box 1 OS=Felis catus GN=RBX1 PE=4 SV=1 gi\|197127417 - gi\|197127417\|gb\|ACH43915.1\| putative RBX1 protein [Taeniopygia guttata] gi\|149743062 - gi\|149743062\|ref\|XP_001502369.1\| PREDICTED: similar to RING-box protein 1 (Rbx1) (Regulator of culli...	8.0E-21	[21..120]	[8..107]
gi\|19067884, gi\|... - gi\|62869880\|gb\|AAY18085.1\| hyperosmotic protein 21 [Salmo salar], gi\|62869878\|gb\|AAY18084.1\| hyperos...	8.0E-21	[17..120]	[83..188]
gi\|12833653 - gi\|12833653\|dbj\|BAB22612.1\| unnamed protein product [Mus musculus]	2.0E-20	[21..120]	[8..107]
gi\|195347430, gi... - gi\|195347430\|ref\|XP_002040256.1\| GM19082 [Drosophila sechellia], gi\|194121684\|gb\|EDW43727.1\| GM19082... FBpp0310165, Roc... - The gene Roc1a is referred to in FlyBase by the symbol Dmel\Roc1a (CG16982, FBgn0025638). It is a pr... gi\|195564465, gi... - gi\|195564465\|ref\|XP_002105838.1\| GD16520 [Drosophila simulans], gi\|194203200\|gb\|EDX16776.1\| GD16520 ... gi\|220952868 - gi\|220952868\|gb\|ACL88977.1\| Roc1a-PA [synthetic construct]	2.0E-20	[8..120]	[1..107]

Back

Predicted Domain #1

Region A:
Residues: [1-121]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSNEVDRMDV DEDESQNIAQ SSNQSAPVET KKKRFEIKKW TAVAFWSWDI AVDNCAICRN  60
   61 HIMEPCIECQ PKAMTDTDNE CVAAWGVCNH AFHLHCINKW IKTRDACPLD NQPWQLARCG 120
  121 R

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	309.30103
Match:	1ldjB_
Description:	RIGG-box protein 1 (RBX1) of SCF ubiquitin ligase complex
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ubiquitin-protein ligase activity	10.2497392096259	bayes_pls_golite062009
small conjugating protein ligase activity	10.0460587929138	bayes_pls_golite062009
acid-amino acid ligase activity	8.99384576623969	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	7.6988425139962	bayes_pls_golite062009
ligase activity	3.92677358871643	bayes_pls_golite062009
binding	2.78710909602478	bayes_pls_golite062009
NEDD8 ligase activity	2.41658628840288	bayes_pls_golite062009
protein binding	1.5975811408548	bayes_pls_golite062009
small conjugating protein-specific protease activity	1.2126425713931	bayes_pls_golite062009
ubiquitin-specific protease activity	1.03469202541773	bayes_pls_golite062009
transcription regulator activity	0.943706743211193	bayes_pls_golite062009
nucleic acid binding	0.708499177773075	bayes_pls_golite062009
hydrolase activity	0.52386813728704	bayes_pls_golite062009
cysteine-type peptidase activity	0.50197530717959	bayes_pls_golite062009
DNA binding	0.365874679056682	bayes_pls_golite062009
ubiquitin thiolesterase activity	0.265769062717221	bayes_pls_golite062009
transcription factor binding	0.16916169785605	bayes_pls_golite062009
catalytic activity	0.14112008370434	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: