YRC Public Data Repository - Protein Structure Prediction

Protein:	ILV2
Organism:	Saccharomyces cerevisiae
Length:	687 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for ILV2.

Description	E-value	Query Range	Subject Range
ILV2 - Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... ILVB_YEAST - Acetolactate synthase catalytic subunit, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 2045... gi\|151946264 - gi\|151946264\|gb\|EDN64495.1\| acetolactate synthase [Saccharomyces cerevisiae YJM789] gi\|207342316 - gi\|207342316\|gb\|EDZ70109.1\| YMR108Wp-like protein [Saccharomyces cerevisiae AWRI1631]	0.0	[1..687]	[1..687]
gi\|17232105, gi\|... - gi\|17232105\|ref\|NP_488653.1\| acetolactate synthase 3 catalytic subunit [Nostoc sp. PCC 7120], gi\|171... gi\|25291313 - pir\|\|AE2382 acetohydroxy acid synthase [imported] - Nostoc sp. (strain PCC 7120)	0.0	[66..678]	[5..608]
gi\|3820612 - gi\|3820612\|gb\|AAC69629.1\| herbicide resistant acetolactate synthase precursor [Bassia scoparia]	0.0	[6..665]	[14..657]
gi\|1314832 - gi\|1314832\|gb\|AAB67839.1\| acetolactate synthase precursor [Amaranthus sp.]	0.0	[12..665]	[18..656]
gi\|7436698 - pir\|\|C70341 acetolactate synthase (EC 4.1.3.18) large chain - Aquifex aeolicus gi\|15605942, gi\|... - gi\|2983101\|gb\|AAC06706.1\| acetolactate synthase large subunit [Aquifex aeolicus VF5], gi\|15605942\|re...	0.0	[93..680]	[5..582]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	0.0	[82..672]	[24..604]
Q66EL8\|Q66EL8_YERPS - Acetolactate synthase III, valine sensitive, large subunit FAD and thiamine PPi binding - Yersinia p... gi\|77631655, gi\|... - gi\|77631655\|ref\|ZP_00794241.1\| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synth... gi\|161511301 - gi\|161511301\|ref\|NP_994917.2\| acetolactate synthase 3 catalytic subunit [Yersinia pestis biovar Micr... gi\|161484748 - gi\|161484748\|ref\|NP_670937.2\| acetolactate synthase 3 catalytic subunit [Yersinia pestis KIM] gi\|186894031, gi... - gi\|186894031\|ref\|YP_001871143.1\| acetolactate synthase 3 catalytic subunit [Yersinia pseudotuberculo... gi\|169752280, gi... - gi\|170025746\|ref\|YP_001722251.1\| acetolactate synthase 3 catalytic subunit [Yersinia pseudotuberculo... gi\|229837069, gi... - gi\|229837069\|ref\|ZP_04457234.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis Pes... gi\|150260425, gi... - gi\|150260425\|ref\|ZP_01917153.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis CA8... gi\|229696794, gi... - gi\|229840428\|ref\|ZP_04460587.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis bio... gi\|229843030, gi... - gi\|229843030\|ref\|ZP_04463180.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis bio... gi\|89104759 - gi\|89104759\|ref\|ZP_01177293.1\| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synth... gi\|108809551, gi... - gi\|108809551\|ref\|YP_653467.1\| acetolactate synthase 3 catalytic subunit [Yersinia pestis Antiqua], g... gi\|77633509 - gi\|77633509\|ref\|ZP_00795637.1\| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synth... gi\|229682102, gi... - gi\|229900763\|ref\|ZP_04515887.1\| acetolactate synthase isozyme III large subunit [Yersinia pestis Nep... gi\|25291303 - pir\|\|AI0066 acetolactate synthase (EC 4.1.3.18) isozyme III large chain [imported] - Yersinia pestis... gi\|218927736, gi... - gi\|218927736\|ref\|YP_002345611.1\| acetolactate synthase 3 catalytic subunit [Yersinia pestis CO92], g... gi\|51588307, gi\|... - gi\|51595025\|ref\|YP_069216.1\| acetolactate synthase 3 catalytic subunit [Yersinia pseudotuberculosis ...	0.0	[92..672]	[4..575]
gi\|6478277 - gi\|6478277\|gb\|AAF13787.1\|AF129501_2 acetohydroxy acid synthase large subunit [Buchnera aphidicola]	0.0	[92..670]	[4..569]
ILVB3_BRANA - Acetolactate synthase 3, chloroplastic OS=Brassica napus PE=3 SV=1	0.0	[26..673]	[6..651]

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Predicted Domain #1

Region A:
Residues: [1-274]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIRQSTLKNF AIKRCFQHIA YRNTPAMRSV ALAQRFYSSS SRYYSASPLP ASKRPEPAPS  60
   61 FNVDPLEQPA EPSKLAKKLR AEPDMDTSFV GLTGGQIFNE MMSRQNVDTV FGYPGGAILP 120
  121 VYDAIHNSDK FNFVLPKHEQ GAGHMAEGYA RASGKPGVVL VTSGPGATNV VTPMADAFAD 180
  181 GIPMVVFTGQ VPTSAIGTDA FQEADVVGIS RSCTKWNVMV KSVEELPLRI NEAFEIATSG 240
  241 RPGPVLVDLP KDVTAAILRN PIPTKTTLPS NALN

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	11000.0
Match:	1jscA_
Description:	Acetohydroxyacid synthase catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
acetolactate synthase activity	12.903449876837	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	12.3239025230705	bayes_pls_golite062009
pyruvate dehydrogenase activity	5.28857071459273	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor	5.21233670052586	bayes_pls_golite062009
pyruvate dehydrogenase (acetyl-transferring) activity	5.08663245333808	bayes_pls_golite062009
transcription regulator activity	2.51117258094456	bayes_pls_golite062009
transketolase activity	2.50501741089295	bayes_pls_golite062009
catalytic activity	2.25537597692103	bayes_pls_golite062009
oxidoreductase activity, acting on the aldehyde or oxo group of donors	2.15444163802756	bayes_pls_golite062009
DNA binding	2.09930834951157	bayes_pls_golite062009
deoxyhypusine synthase activity	2.04735838056767	bayes_pls_golite062009
nucleic acid binding	2.00972611627502	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	1.98546381612935	bayes_pls_golite062009
NAD-dependent protein deacetylase activity	1.93141177609772	bayes_pls_golite062009
NAD-dependent histone deacetylase activity	1.93141177609772	bayes_pls_golite062009
histone deacetylase activity	1.91254869751164	bayes_pls_golite062009
protein deacetylase activity	1.90521776801103	bayes_pls_golite062009
thiamin pyrophosphate binding	1.87247313698582	bayes_pls_golite062009
oxidoreductase activity	1.85833680148029	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.82969293063325	bayes_pls_golite062009
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity	1.65348126142808	bayes_pls_golite062009
alpha-ketoacid dehydrogenase activity	1.20517690263403	bayes_pls_golite062009
binding	1.17940168894417	bayes_pls_golite062009
transcription factor binding	1.07103864164531	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	1.0611726850452	bayes_pls_golite062009
transcription factor activity	1.0180828203704	bayes_pls_golite062009
NAD or NADH binding	0.98460927780383	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.982646671777	bayes_pls_golite062009
deacetylase activity	0.977680867213479	bayes_pls_golite062009
cation transmembrane transporter activity	0.78591260444574	bayes_pls_golite062009
hydrolase activity	0.536729211484905	bayes_pls_golite062009
transcription repressor activity	0.516307124442946	bayes_pls_golite062009
ion transmembrane transporter activity	0.51426028502998	bayes_pls_golite062009
transcription cofactor activity	0.42635585193838	bayes_pls_golite062009
transferase activity	0.413024577830522	bayes_pls_golite062009
transcription corepressor activity	0.23414654967509	bayes_pls_golite062009
pyruvate decarboxylase activity	0.198062360371269	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	0.114062221123972	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [275-460]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QLTSRAQDEF VMQSINKAAD LINLAKKPVL YVGAGILNHA DGPRLLKELS DRAQIPVTTT  60
   61 LQGLGSFDQE DPKSLDMLGM HGCATANLAV QNADLIIAVG ARFDDRVTGN ISKFAPEARR 120
  121 AAAEGRGGII HFEVSPKNIN KVVQTQIAVE GDATTNLGKM MSKIFPVKER SEWFAQINKW 180
  181 KKEYPY

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [510-522]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 WRNPHTFITS GGL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	11000.0
Match:	1jscA_
Description:	Acetohydroxyacid synthase catalytic subunit
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [461-509]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AYMEETPGSK IKPQTVIKKL SKVANDTGRH VIVTTGVGQH QMWAAQHWT

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [523-687]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GTMGYGLPAA IGAQVAKPES LVIDIDGDAS FNMTLTELSS AVQAGTPVKI LILNNEEQGM  60
   61 VTQWQSLFYE HRYSHTHQLN PDFIKLAEAM GLKGLRVKKQ EELDAKLKEF VSTKGPVLLE 120
  121 VEVDKKVPVL PMVAGGSGLD EFINFDPEVE RQQTELRHKR TGGKH

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	11000.0
Match:	1jscA_
Description:	Acetohydroxyacid synthase catalytic subunit
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: