YRC Public Data Repository - Protein Structure Prediction

Protein:	TOK1
Organism:	Saccharomyces cerevisiae
Length:	691 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for TOK1.

Description	E-value	Query Range	Subject Range
TOK1_YEAST - Outward-rectifier potassium channel TOK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN... gi\|190409408 - gi\|190409408\|gb\|EDV12673.1\| outward-rectifier potassium channel TOK1 [Saccharomyces cerevisiae RM11-... TOK1 - Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of ...	0.0	[1..691]	[1..691]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	6.0E-85	[18..344]	[158..487]
gi\|225892 - gi\|225892\|prf\|\|1402312A K channel protein gi\|288442, gi\|13... - gi\|288442\|emb\|CAA29917.1\| Shaker [Drosophila melanogaster], gi\|13432103\|sp\|P08510\|KCNAS_DROME Potass...	1.0E-84	[18..344]	[170..499]
CIK3_MOUSE, KCNA... - Potassium voltage-gated channel subfamily A member 3 - Mus musculus (Mouse), (P16390) Potassium volt...	2.0E-83	[18..354]	[130..464]
KCNA5_MUSPF - Potassium voltage-gated channel subfamily A member 5 OS=Mustela putorius furo GN=KCNA5 PE=2 SV=1	7.0E-83	[18..331]	[182..512]
gi\|114559003, gi... - gi\|114559003\|ref\|XP_001162925.1\| PREDICTED: potassium voltage-gated channel, shaker-related subfamil... gi\|208968679 - gi\|208968679\|dbj\|BAG74178.1\| potassium voltage-gated channel, shaker-related subfamily, member 2 [sy... KCNA2 - potassium voltage-gated channel, shaker-related subfamily, member 2 gi\|61841397 - gi\|61841397\|ref\|XP_588328.1\| PREDICTED: similar to potassium channel [Bos taurus]	2.0E-82	[18..362]	[106..449]

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Predicted Domain #1

Region A:
Residues: [1-117]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTRFMNSFAK QTLGYGNMAT VEQESSAQAV DSHSNNTPKQ AKGVLAEELK DALRFRDERV  60
   61 SIINAEPSST LFVFWFVVSC YFPVITACLG PVANTISIAC VVEKWRSLKN NSVVTNP

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [118-194]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RSNDTDVLMN QVKTVFDPPG IFAVNIISLV LGFTSNIILM LHFSKKLTYL KSQLINITGW  60
   61 TIAGGMLLVD VIVCSLN

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
potassium channel activity	6.80950139957332	bayes_pls_golite062009
voltage-gated potassium channel activity	6.78740036921969	bayes_pls_golite062009
voltage-gated cation channel activity	6.69340621375858	bayes_pls_golite062009
voltage-gated ion channel activity	6.55904318827964	bayes_pls_golite062009
voltage-gated channel activity	6.55320598344395	bayes_pls_golite062009
metal ion transmembrane transporter activity	5.06264960439093	bayes_pls_golite062009
cation transmembrane transporter activity	4.86252983188139	bayes_pls_golite062009
cation channel activity	4.85749153259346	bayes_pls_golite062009
ion transmembrane transporter activity	4.77144021609018	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	4.3961579058414	bayes_pls_golite062009
gated channel activity	4.19619757426613	bayes_pls_golite062009
inward rectifier potassium channel activity	4.13359972196575	bayes_pls_golite062009
substrate-specific channel activity	4.09858664479122	bayes_pls_golite062009
ion channel activity	3.86720832958006	bayes_pls_golite062009
channel activity	3.7587607940377	bayes_pls_golite062009
passive transmembrane transporter activity	3.7587607940377	bayes_pls_golite062009
transporter activity	2.93091397928047	bayes_pls_golite062009
transmembrane transporter activity	2.82224868778929	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	2.63886826791804	bayes_pls_golite062009
substrate-specific transporter activity	2.54207434157938	bayes_pls_golite062009
ligand-gated ion channel activity	2.26062383880472	bayes_pls_golite062009
ligand-gated channel activity	2.26062383880472	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	1.88952785751441	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	1.58358846593395	bayes_pls_golite062009
potassium ion transmembrane transporter activity	1.57069429249737	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	0.282641904073821	bayes_pls_golite062009
active transmembrane transporter activity	0.239024094835991	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	0.22529291758658	bayes_pls_golite062009
binding	0.144628283348863	bayes_pls_golite062009

Predicted Domain #3

Region A:
Residues: [195-350]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DMPSIYSKTI GFWFACISSG LYLVCTIILT IHFIGYKLGK YPPTFNLLPN ERSIMAYTVL  60
   61 LSLWLIWGAG MFSGLLHITY GNALYFCTVS LLTVGLGDIL PKSVGAKIMV LIFSLSGVVL 120
  121 MGLIVFMTRS IIQKSSGPIF FFHRVEKGRS KSWKHY

[Run NCBI BLAST on this sequence.]

Detection Method:	ORFEUS
Confidence:	10.57
Match:	1f6gA
Description:	Potassium channel protein
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [351-458]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDSSKNLSER EAFDLMKCIR QTASRKQHWF SLSVTIAIFM AFWLLGALVF KFAENWSYFN  60
   61 CIYFCFLCLL TIGYGDYAPR TGAGRAFFVI WALGAVPLMG AILSTVGD

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	57.512579
Match:	1lnqA_
Description:	Potassium channel-related protein MthK
Matching Structure (courtesy of the PDB):

Predicted Domain #5

Region A:
Residues: [459-638]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LLFDISTSLD IKIGESFNNK VKSIVFNGRQ RALSFMVNTG EIFEESDTAD GDLEENTTSS  60
   61 QSSQISEFND NNSEENDSGV TSPPASLQES FSSLSKASSP EGILPLEYVS SAEYALQDSG 120
  121 TCNLRNLQEL LKAVKKLHRI CLADKDYTLS FSDWSYIHKL HLRNITDIEE YTRGPEFWIS 180
  181

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	57.512579
Match:	1lnqA_
Description:	Potassium channel-related protein MthK
Matching Structure (courtesy of the PDB):

Predicted Domain #6

Region A:
Residues: [639-691]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PDTPLKFPLN EPHFAFMMLF KNIEELVGNL VEDEELYKVI SKRKFLGEHR KTL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: