YRC Public Data Repository - Protein Structure Prediction

Protein:	NAS2
Organism:	Saccharomyces cerevisiae
Length:	220 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NAS2.

Description	E-value	Query Range	Subject Range
gi\|229344317, gi... - gi\|229519628\|ref\|ZP_04409071.1\| outer membrane stress sensor protease DegQ [Vibrio cholerae RC9], gi... gi\|229342095, gi... - gi\|229520862\|ref\|ZP_04410284.1\| outer membrane stress sensor protease DegQ [Vibrio cholerae TM 11079... gi\|227080749, gi... - gi\|227080749\|ref\|YP_002809300.1\| protease DO [Vibrio cholerae M66-2], gi\|227008637\|gb\|ACP04849.1\| pr... gi\|229507177, gi... - gi\|229507177\|ref\|ZP_04396683.1\| outer membrane stress sensor protease DegQ [Vibrio cholerae BX 33028... gi\|15640588, gi\|... - gi\|9654996\|gb\|AAF93734.1\| protease DO [Vibrio cholerae O1 biovar El Tor str. N16961], gi\|15640588\|re... gi\|11278798 - pir\|\|F82307 proteinase DO VC0566 [imported] - Vibrio cholerae (strain N16961 serogroup O1) gi\|229606145, gi... - gi\|229606145\|ref\|YP_002876793.1\| outer membrane stress sensor protease DegQ [Vibrio cholerae MJ-1236... gi\|121591583, gi... - gi\|121591583\|ref\|ZP_01678838.1\| protease DO [Vibrio cholerae 2740-80], gi\|121546565\|gb\|EAX56765.1\| p... gi\|124120842, gi... - gi\|153803624\|ref\|ZP_01958210.1\| protease DO [Vibrio cholerae MZO-3], gi\|124120842\|gb\|EAY39585.1\| pro... gi\|153224050, gi... - gi\|153224050\|ref\|ZP_01954195.1\| protease DO [Vibrio cholerae MAK 757], gi\|124117870\|gb\|EAY36613.1\| p... gi\|126518367, gi... - gi\|229509162\|ref\|ZP_04398647.1\| outer membrane stress sensor protease DegQ [Vibrio cholerae B33], gi... gi\|125617304 - gi\|125617304\|gb\|EAZ45876.1\| protease DO [Vibrio cholerae MO10] gi\|153830227, gi... - gi\|153830227\|ref\|ZP_01982894.1\| protease DO [Vibrio cholerae 623-39], gi\|148874287\|gb\|EDL72422.1\| pr... gi\|147673156, gi... - gi\|227116942\|ref\|YP_002818838.1\| protease DO [Vibrio cholerae O395], gi\|227012392\|gb\|ACP08602.1\| pro...	2.0E-45	[2..212]	[159..368]
gi\|12721029, gi\|... - gi\|15602599\|ref\|NP_245671.1\| hypothetical protein PM0734 [Pasteurella multocida subsp. multocida str...	7.0E-45	[2..206]	[162..366]
gi\|2935166 - gi\|2935166\|gb\|AAC38202.1\| HtrA [Haemophilus influenzae]	9.0E-44	[2..210]	[140..348]
gi\|7445360 - pir\|\|F72359 periplasmic serine proteinase Do (EC 3.4.21.-) - Thermotoga maritima (strain MSB8) gi\|15643337, gi\|... - gi\|4981088\|gb\|AAD35656.1\|AE001732_1 heat shock serine protease, periplasmic [Thermotoga maritima MSB...	1.0E-43	[2..202]	[147..348]
EG10463 - serine endoprotease (protease Do), membrane-associated / Periplasmic, membrane-associated serine end... gi\|238863539, gi... - gi\|238899560\|ref\|YP_002925356.1\| serine endoprotease (protease Do), membrane-associated [Escherichia... gi\|253976391, gi... - gi\|254287084\|ref\|YP_003052832.1\| serine endoprotease (protease Do), membrane-associated [Escherichia... gi\|226840687, gi... - gi\|254037581\|ref\|ZP_04871658.1\| serine endoprotease [Escherichia sp. 1_1_43], gi\|226840687\|gb\|EEH726... DEGP_ECO57 - Periplasmic serine endoprotease DegP OS=Escherichia coli O157:H7 GN=degP PE=3 SV=1 gi\|254160281, gi... - gi\|254160281\|ref\|YP_003043389.1\| serine endoprotease (protease Do), membrane-associated [Escherichia... EG10463 - serine endoprotease (protease Do), membrane-associated / Periplasmic, membrane-associated serine end... gi\|189365646, gi... - gi\|189365646\|gb\|EDU84062.1\| protease Do [Escherichia coli O157:H7 str. EC4501], gi\|168764331\|ref\|ZP_... gi\|188487913, gi... - gi\|75512174\|ref\|ZP_00734746.1\| COG0265: Trypsin-like serine proteases, typically periplasmic, contai... gi\|170021485, gi... - gi\|170021485\|ref\|YP_001726439.1\| serine endoprotease [Escherichia coli ATCC 8739], gi\|169756413\|gb\|A... gi\|74310782, gi\|... - gi\|74310782\|ref\|YP_309201.1\| serine endoprotease [Shigella sonnei Ss046], gi\|73854259\|gb\|AAZ86966.1\|... gi\|168789271, gi... - gi\|189371084\|gb\|EDU89500.1\| protease Do [Escherichia coli O157:H7 str. EC869], gi\|168789271\|ref\|ZP_0... gi\|75176493, gi\|... - gi\|75176493\|ref\|ZP_00696630.1\| COG0265: Trypsin-like serine proteases, typically periplasmic, contai... gi\|217324151, gi... - gi\|217324151\|ref\|ZP_03440235.1\| protease Do [Escherichia coli O157:H7 str. TW14588], gi\|217320372\|gb... gi\|190903575, gi... - gi\|75230476\|ref\|ZP_00716957.1\| COG0265: Trypsin-like serine proteases, typically periplasmic, contai... gi\|169887633, gi... - gi\|170079798\|ref\|YP_001729118.1\| serine endoprotease (protease Do), membrane-associated [Escherichia... gi\|218430514, gi... - gi\|218703417\|ref\|YP_002410936.1\| serine endoprotease [Escherichia coli UMN026], gi\|218430514\|emb\|CAR... gi\|82542761, gi\|... - gi\|82542761\|ref\|YP_406708.1\| serine endoprotease [Shigella boydii Sb227], gi\|81244172\|gb\|ABB64880.1\|... gi\|15799845, gi\|... - gi\|15799845\|ref\|NP_285857.1\| serine endoprotease [Escherichia coli O157:H7 EDL933], gi\|12512885\|gb\|A... gi\|21239017, gi\|... - gi\|89107042\|ref\|AP_000822.1\| serine endoprotease (protease Do), membrane-associated [Escherichia col... gi\|124529614, gi... - gi\|124529614\|ref\|ZP_01700558.1\| protease Do [Escherichia coli B], gi\|124499770\|gb\|EAY47253.1\| protea... DEGP_ECOLI - Protease do precursor (EC 3.4.21.-) - Escherichia coli gi\|13359621, gi\|... - gi\|15829419\|ref\|NP_308192.1\| serine endoprotease [Escherichia coli O157:H7 str. Sakai], gi\|13359621\|... gi\|194418374, gi... - gi\|83569742\|ref\|ZP_00921192.1\| COG0265: Trypsin-like serine proteases, typically periplasmic, contai... gi\|194439111, gi... - gi\|83585478\|ref\|ZP_00924123.1\| COG0265: Trypsin-like serine proteases, typically periplasmic, contai...	2.0E-43	[2..208]	[180..385]
HTOA_HAEIN - Probable periplasmic serine protease do/hhoA-like OS=Haemophilus influenzae GN=HI_1259 PE=3 SV=1 HTOA_HAEIN - Probable periplasmic serine protease do/HhoA-like OS=Haemophilus influenzae (strain ATCC 51907 / DSM...	2.0E-43	[2..206]	[169..373]
gi\|7259285 - gi\|7259285\|dbj\|BAA92745.1\| heat shock protein HtrA [Shigella sonnei]	5.0E-43	[2..208]	[180..385]
gi\|7507776 - gi\|7507776\|pir\|\|T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans	8.0E-43	[2..208]	[180..385]
gi\|25305541, gi\|... - gi\|25305541\|pir\|\|AC0528 protease DO precursor, heat shock protein HtrA [imported] - Salmonella enter... gi\|29140750, gi\|... - gi\|29140750\|ref\|NP_804092.1\| serine endoprotease [Salmonella enterica subsp. enterica serovar Typhi ... gi\|213051748 - gi\|213051748\|ref\|ZP_03344626.1\| serine endoprotease [Salmonella enterica subsp. enterica serovar Typ... gi\|16759200 - gi\|16759200\|ref\|NP_454817.1\| serine endoprotease [Salmonella enterica subsp. enterica serovar Typhi ... gi\|213425935 - gi\|213425935\|ref\|ZP_03358685.1\| serine endoprotease [Salmonella enterica subsp. enterica serovar Typ... gi\|213852252 - gi\|213852252\|ref\|ZP_03381784.1\| serine endoprotease [Salmonella enterica subsp. enterica serovar Typ...	2.0E-42	[2..203]	[181..381]

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Predicted Domain #1

Region A:
Residues: [1-220]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS VLEQQGIGMD  60
   61 SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS HVLLNQHFDN MNVKSNQDAR 120
  121 RNNDDQAIQY TIPFAFISEV VPGSPSDKAD IKVDDKLISI GNVHAANHSK LQNIQMVVMK 180
  181 NEDRPLPVLL LREGQILKTS LTPSRNWNGR GLLGCRIQEL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	42.0
Match:	1ky9A_
Description:	Protease Do (DegP, HtrA), C-terminal domains; Protease Do (DegP, HtrA), catalytic domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
peptidase activity	4.00482245922158	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	3.80641317919152	bayes_pls_golite062009
endopeptidase activity	3.69721977392816	bayes_pls_golite062009
protein binding	3.40921081688513	bayes_pls_golite062009
binding	3.2595117613307	bayes_pls_golite062009
hydrolase activity	2.69939003844937	bayes_pls_golite062009
transcription regulator activity	2.16834620509051	bayes_pls_golite062009
transcription activator activity	1.37255187012662	bayes_pls_golite062009
transporter activity	0.666562078558936	bayes_pls_golite062009
transmembrane transporter activity	0.510205657363692	bayes_pls_golite062009
enzyme binding	0.46071739042312	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.342817187733892	bayes_pls_golite062009
small conjugating protein ligase activity	0.299572882681276	bayes_pls_golite062009
structural molecule activity	0.153277808949873	bayes_pls_golite062009
substrate-specific transporter activity	0.141613198202921	bayes_pls_golite062009
PDZ domain binding	0.0604467745298803	bayes_pls_golite062009
transcription factor binding	0.0283953080227368	bayes_pls_golite062009
cytoskeletal protein binding	0.0187659836234897	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: