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View Structure Prediction Details

Protein: DMA1
Organism: Saccharomyces cerevisiae
Length: 416 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for DMA1.

Description E-value Query
Range
Subject
Range
DMA1 - Protein involved in ubiquitin ligation; plays a role in regulating spindle position and orientation;...
DMA1_YEAST - E3 ubiquitin-protein ligase DMA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DMA1 PE...
gi|190405894 - gi|190405894|gb|EDV09161.1| hypothetical protein SCRG_04828 [Saccharomyces cerevisiae RM11-1a]
1.0E-66 [1..416] [1..416]
gi|4959046, gi|4... - gi|4959046|gb|AAD34210.1|AF069993_1 LIM domain interacting RING finger protein [Gallus gallus], gi|4...
2.0E-39 [9..382] [214..591]
gi|9758583, gi|2... - gi|9758583|dbj|BAB09196.1| unnamed protein product [Arabidopsis thaliana], gi|28416527|gb|AAO42794.1...
3.0E-32 [5..372] [326..679]
dma1 - mitotic spindle checkpoint protein Dma1
DMA1_SCHPO - Probable E3 ubiquitin-protein ligase dma1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=...
3.0E-30 [155..393] [28..258]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
3.0E-28 [5..273] [34..284]
RNF12_MOUSE - E3 ubiquitin-protein ligase RLIM OS=Mus musculus GN=Rlim PE=1 SV=2
3.0E-27 [9..382] [214..598]

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Predicted Domain #1
Region A:
Residues: [1-118]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTNTVPSSP PNQTPPAASG IATSHDHTKF NNPIRLPISI SLTINDTPNN NSNNNSVSNG  60
   61 LGILPSRTAT SLVVANNGSA NGNVGATAAA AATVETNTAP AVNTTKSIRH FIYPPNQV

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.274 b.71.1 Glycosyl hydrolase domain
View Download 0.249 a.39.2 Insect pheromon/odorant-binding proteins

Predicted Domain #2
Region A:
Residues: [119-326]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NQTEFSLDIH LPPNTSLPER IDQSTLKRRM DKHGLFSIRL TPFIDTSSTS VANQGLFFDP  60
   61 IIRTAGAGSQ IIIGRYTERV REAISKIPDQ YHPVVFKSKV ISRTHGCFKV DDQGNWFLKD 120
  121 VKSSSGTFLN HQRLSSASTT SKDYLLHDGD IIQLGMDFRG GTEEIYRCVK MKIELNKSWK 180
  181 LKANAFNKEA LSRIKNLQKL TTGLEQED

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [380-416]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LESESESEFE NEDEDEPDIE MDIDMEINNN LGVRLVD

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 6.0
Match: 1fbvA_
Description: Cbl; N-terminal domain of cbl (N-cbl); CBL
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
cytoskeletal protein binding 3.49668552859347 bayes_pls_golite062009
motor activity 3.25814691068414 bayes_pls_golite062009
binding 2.69791297400248 bayes_pls_golite062009
tubulin binding 2.51210014078446 bayes_pls_golite062009
actin binding 2.38676888633305 bayes_pls_golite062009
ubiquitin-protein ligase activity 2.20438586677555 bayes_pls_golite062009
microtubule binding 2.17136406283012 bayes_pls_golite062009
protein binding 2.14586566310919 bayes_pls_golite062009
small conjugating protein ligase activity 1.97264874084155 bayes_pls_golite062009
molecular adaptor activity 1.33740973673697 bayes_pls_golite062009
structural constituent of muscle 1.30669499755555 bayes_pls_golite062009
acid-amino acid ligase activity 1.26357595913158 bayes_pls_golite062009
SH3/SH2 adaptor activity 1.2323381312171 bayes_pls_golite062009
microfilament motor activity 1.0725773039399 bayes_pls_golite062009
signal transducer activity 0.999269271132931 bayes_pls_golite062009
molecular transducer activity 0.999269271132931 bayes_pls_golite062009
protein binding, bridging 0.83823250804321 bayes_pls_golite062009
actin filament binding 0.836375875773328 bayes_pls_golite062009
structural molecule activity 0.800850121197479 bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor 0.748479359639348 bayes_pls_golite062009
kinase activity 0.662911740280035 bayes_pls_golite062009
N-terminal myristoylation domain binding 0.63045154489014 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 0.493362981045534 bayes_pls_golite062009
G-protein beta/gamma-subunit binding 0.487272307313601 bayes_pls_golite062009
enzyme regulator activity 0.456661966897198 bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds 0.452350351194157 bayes_pls_golite062009
protein phosphorylated amino acid binding 0.43808715710519 bayes_pls_golite062009
nucleoside-triphosphatase activity 0.382055332967642 bayes_pls_golite062009
enzyme activator activity 0.369738999181035 bayes_pls_golite062009
actin-dependent ATPase activity 0.3688716707761 bayes_pls_golite062009
pyrophosphatase activity 0.321819287555157 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides 0.30564870400158 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides 0.305174269756747 bayes_pls_golite062009
kinase regulator activity 0.262406945568467 bayes_pls_golite062009
transferase activity 0.169970258787201 bayes_pls_golite062009
phosphoprotein binding 0.16798254471727 bayes_pls_golite062009
myosin binding 0.125186564742711 bayes_pls_golite062009
structural constituent of cytoskeleton 0.116648335890738 bayes_pls_golite062009
protein kinase activity 0.0865488336042732 bayes_pls_golite062009

Predicted Domain #3
Region A:
Residues: [327-379]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 CSICLNKIKP CQAIFISPCA HSWHFHCVRR LVIMNYPQFM CPNCRTNCDL ETT

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 6.0
Match: 1fbvA_
Description: Cbl; N-terminal domain of cbl (N-cbl); CBL
Matching Structure (courtesy of the PDB):

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle