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View Structure Prediction Details

Protein: RAD30
Organism: Saccharomyces cerevisiae
Length: 632 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for RAD30.

Description E-value Query
Range
Subject
Range
gi|207346313 - gi|207346313|gb|EDZ72843.1| YDR419Wp-like protein [Saccharomyces cerevisiae AWRI1631]
gi|190404649 - gi|190404649|gb|EDV07916.1| DNA polymerase eta [Saccharomyces cerevisiae RM11-1a]
POLH_YEAST - DNA polymerase eta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD30 PE=1 SV=1
RAD30 - DNA polymerase eta, involved in tranlesion synthesis during post-replication repair; catalyzes the s...
0.0 [1..632] [1..632]
POLH - polymerase (DNA directed), eta
0.0 [24..624] [7..563]
POLH_MOUSE - DNA polymerase eta OS=Mus musculus GN=Polh PE=1 SV=1
0.0 [23..615] [6..549]

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Predicted Domain #1
Region A:
Residues: [1-33]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKFTWKELI QLGSPSKAYE SSLACIAHID MNA

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [122-299]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EDHKVSLEPY RRESRKALKI FKSACDLVER ASIDEVFLDL GRICFNMLMF DNEYELTGDL  60
   61 KLKDALSNIR EAFIGGNYDI NSHLPLIPEK IKSLKFEGDV FNPEGRDLIT DWDDVILALG 120
  121 SQVCKGIRDS IKDILGYTTS CGLSSTKNVC KLASNYKKPD AQTIVKNDCL LDFLDCGK

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 10119.0
Match: 1jihA_
Description: DNA polymerase eta
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
DNA-directed DNA polymerase activity 8.5725258398851 bayes_pls_golite062009
DNA polymerase activity 8.52309706572764 bayes_pls_golite062009
nucleotidyltransferase activity 6.34496739872612 bayes_pls_golite062009
DNA primase activity 5.34779288251618 bayes_pls_golite062009
chromatin binding 2.18085270555452 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 1.74380808085854 bayes_pls_golite062009
DNA binding 1.69096430884207 bayes_pls_golite062009
nucleic acid binding 1.57540655259696 bayes_pls_golite062009
binding 1.40175931463327 bayes_pls_golite062009
structure-specific DNA binding 1.06010018021303 bayes_pls_golite062009
catalytic activity 1.04899053677564 bayes_pls_golite062009
DNA-directed RNA polymerase activity 0.83818211151495 bayes_pls_golite062009
RNA polymerase activity 0.83818211151495 bayes_pls_golite062009
transferase activity 0.784455099927681 bayes_pls_golite062009
exonuclease activity 0.778608024896469 bayes_pls_golite062009
damaged DNA binding 0.39441061793492 bayes_pls_golite062009
protein binding 0.354190470641287 bayes_pls_golite062009
3'-5' exonuclease activity 0.0700154911930899 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [34-121]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FFAQVEQMRC GLSKEDPVVC VQWNSIIAVS YAARKYGISR MDTIQEALKK CSNLIPIHTA  60
   61 VFKKGEDFWQ YHDGCGSWVQ DPAKQISV

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [300-388]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FEITSFWTLG GVLGKELIDV LDLPHENSIK HIRETWPDNA GQLKEFLDAK VKQSDYDRST  60
   61 SNIDPLKTAD LAEKLFKLSR GRYGLPLSS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 10119.0
Match: 1jihA_
Description: DNA polymerase eta
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
transcription regulator activity 3.3375695352073 bayes_pls_golite062009
DNA binding 3.28852346568913 bayes_pls_golite062009
nucleic acid binding 3.28464324017293 bayes_pls_golite062009
binding 2.56809202445862 bayes_pls_golite062009
structure-specific DNA binding 2.42858975345257 bayes_pls_golite062009
transcription factor activity 2.32991522163341 bayes_pls_golite062009
chromatin binding 2.32872787634324 bayes_pls_golite062009
transcription activator activity 1.20800295439208 bayes_pls_golite062009
RNA polymerase II transcription factor activity 1.14039305317011 bayes_pls_golite062009
protein binding 0.952480850696098 bayes_pls_golite062009
double-stranded DNA binding 0.752135837564561 bayes_pls_golite062009
transcription repressor activity 0.733541244206639 bayes_pls_golite062009
sequence-specific DNA binding 0.564341752288536 bayes_pls_golite062009
transcription factor binding 0.547857728784058 bayes_pls_golite062009
catalytic activity 0.263007611601737 bayes_pls_golite062009

Predicted Domain #3
Region A:
Residues: [389-530]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RPVVKSMMSN KNLRGKSCNS IVDCISWLEV FCAELTSRIQ DLEQEYNKIV IPRTVSISLK  60
   61 TKSYEVYRKS GPVAYKGINF QSHELLKVGI KFVTDLDIKG KNKSYYPLTK LSMTITNFDI 120
  121 IDLQKTVVDM FGNQVHTFKS SA

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 10119.0
Match: 1jihA_
Description: DNA polymerase eta
Matching Structure (courtesy of the PDB):

Predicted Domain #4
Region A:
Residues: [531-632]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GKEDEEKTTS SKADEKTPKL ECCKYQVTFT DQKALQEHAD YHLALKLSEG LNGAEESSKN  60
   61 LSFGEKRLLF SRKRPNSQHT ATPQKKQVTS SKNILSFFTR KK

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle