YRC Public Data Repository - Protein Structure Prediction

Protein:	MUS81
Organism:	Saccharomyces cerevisiae
Length:	632 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MUS81.

Description	E-value	Query Range	Subject Range
gi\|190404676 - gi\|190404676\|gb\|EDV07943.1\| class II crossover junction endonuclease MUS81 [Saccharomyces cerevisiae... MUS81_YEAST - Crossover junction endonuclease MUS81 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MU... MUS81 - Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in D...	0.0	[1..632]	[1..632]
gi\|19075333 - gi\|19075333\|ref\|NP_587833.1\| hypothetical protein SPCC4G3.05c [Schizosaccharomyces pombe 972h-] gi\|46396277, gi\|... - gi\|7491907\|pir\|\|T41371 hypothetical protein SPCC4G3.05c - fission yeast (Schizosaccharomyces pombe)...	0.0	[40..631]	[1..570]
gi\|15242470 - gi\|15242470\|ref\|NP_198793.1\| repair endonuclease family protein [Arabidopsis thaliana]	4.0E-98	[21..606]	[764..1207]
gi\|193785222, gi... - gi\|46396495\|sp\|Q96NY9\|MUS81_HUMAN Crossover junction endonuclease MUS81 homolog (Holliday junction r...	2.0E-78	[10..247]	[20..242]
mus-81 - status:Partially_confirmed UniProt:P91153 protein_id:AAB37627.1	6.0E-75	[214..617]	[67..439]
gi\|19352281 - gi\|19352281\|gb\|AAL86954.1\|AC114259_1 similar to Homo sapiens (Human). MUS81 endonuclease [Dictyostel...	8.0E-67	[224..629]	[575..957]
gi\|220953062 - gi\|220953062\|gb\|ACL89074.1\| mus81-PA [synthetic construct] mus81-PA - The gene mus81 is referred to in FlyBase by the symbol Dmel\mus81 (CG3026, FBgn0040347). It is a pro...	2.0E-66	[280..623]	[93..419]
gi\|18894225, gi\|... - gi\|18978387\|ref\|NP_579744.1\| Hef nuclease [Pyrococcus furiosus DSM 3638], gi\|18894225\|gb\|AAL82139.1\|...	1.0E-63	[287..585]	[494..759]
gi\|14591755 - gi\|14591755\|ref\|NP_143722.1\| Hef nuclease [Pyrococcus horikoshii OT3]	2.0E-60	[288..585]	[497..744]

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Predicted Domain #1

Region A:
Residues: [1-226]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MELSSNLKDL YIEWLQELVD GLTPKQEQLK IAYEKAKRNL QNAEGSFYYP TDLKKVKGIG  60
   61 NTIIKRLDTK LRNYCKIHHI SPVEAPSLTQ TSSTRPPKRT TTALRSIVNS CENDKNEAPE 120
  121 EKGTKKRKTR KYIPKKRSGG YAILLSLLEL NAIPRGVSKE QIIEVAGKYS DHCMTPNFST 180
  181 KEFYGAWSSI AALKKHSLVL EEGRPKRYSL TEEGVELTKS LKTADG

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
DNA-directed DNA polymerase activity	3.46780352518677	bayes_pls_golite062009
DNA polymerase activity	3.42429084792579	bayes_pls_golite062009
nucleotidyltransferase activity	1.8990253905448	bayes_pls_golite062009
catalytic activity	0.585392957294978	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [227-334]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 ISFPKENEEP NEYSVTRNES SEFTANLTDL RGEYGKEEEP CDINNTSFML DITFQDLSTP  60
   61 QRLQNNVFKN DRLNSQTNIS SHKLEEVSDD QTVPDSALKA KSTIKRRR

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #3

Region A:
Residues: [335-454]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YNGVSYELWC SGDFEVFPII DHREIKSQSD REFFSRAFER KGMKSEIRQL ALGDIIWVAK  60
   61 NKNTGLQCVL NTIVERKRLD DLALSIRDNR FMEQKNRLEK SGCEHKYYLI EETMSGNIGN 120
  121

[Run NCBI BLAST on this sequence.]

Detection Method:	Pfam
Confidence:	50.086186
Match:	PF02732
Description:	ERCC4 domain

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted functions:

Term	Confidence	Notes
four-way junction helicase activity	10.562254984301	bayes_pls_golite062009
DNA helicase activity	7.81641089090703	bayes_pls_golite062009
endodeoxyribonuclease activity	3.88121538069074	bayes_pls_golite062009
single-stranded DNA specific endodeoxyribonuclease activity	3.87045877570028	bayes_pls_golite062009
deoxyribonuclease activity	3.62965779224499	bayes_pls_golite062009
excinuclease ABC activity	3.48615352002265	bayes_pls_golite062009
DNA binding	2.97855002479178	bayes_pls_golite062009
structure-specific DNA binding	2.83758378400856	bayes_pls_golite062009
nucleic acid binding	2.79236389919005	bayes_pls_golite062009
single-stranded DNA binding	2.79104877030658	bayes_pls_golite062009
damaged DNA binding	2.3008149920351	bayes_pls_golite062009
helicase activity	2.29491252701513	bayes_pls_golite062009
binding	2.17324322139334	bayes_pls_golite062009
nuclease activity	1.92659045080864	bayes_pls_golite062009
endonuclease activity	1.7109181764667	bayes_pls_golite062009
DNA strand annealing activity	1.5117854648336	bayes_pls_golite062009
pyrophosphatase activity	1.02983374615445	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	1.02325715570644	bayes_pls_golite062009
catalytic activity	0.816447099398022	bayes_pls_golite062009
double-stranded DNA binding	0.73359455482295	bayes_pls_golite062009
protein binding	0.71091195096997	bayes_pls_golite062009

Predicted Domain #4

Region A:
Residues: [455-632]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNEALKTALW VILVYYKFSM IRTCNSDETV EKIHALHTVI SHHYSQKDLI VIFPSDLKSK  60
   61 DDYKKVLLQF RREFERKGGI ECCHNLECFQ ELMGKGDLKT VGELTIHVLM LVKGISLEKA 120
  121 VAIQEIFPTL NKILMAYKTC SSEEEAKLLM FNVLGDAPGA KKITKSLSEK IYDAFGKL

[Run NCBI BLAST on this sequence.]

Detection Method:

MSA

Shown below is our most confident prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions:

Predicted functions: