YRC Public Data Repository - Protein Structure Prediction

Protein:	SIR2
Organism:	Saccharomyces cerevisiae
Length:	562 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for SIR2.

Description	E-value	Query Range	Subject Range
SIR2_YEAST - NAD-dependent histone deacetylase SIR2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=S... SIR2 - Conserved NAD+ dependent histone deacetylase of the Sirtuin family involved in regulation of lifespa... gi\|190405052 - gi\|190405052\|gb\|EDV08319.1\| NAD-dependent histone deacetylase SIR2 [Saccharomyces cerevisiae RM11-1a... gi\|207346927 - gi\|207346927\|gb\|EDZ73271.1\| YDL042Cp-like protein [Saccharomyces cerevisiae AWRI1631]	0.0	[1..562]	[1..562]
SIR2_KLULA - NAD-dependent histone deacetylase SIR2 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70... SIR2_KLULA - NAD-dependent histone deacetylase SIR2 OS=Kluyveromyces lactis GN=SIR2 PE=3 SV=2	0.0	[99..554]	[142..610]
SIR2_CANAL - NAD-dependent histone deacetylase SIR2 OS=Candida albicans GN=SIR2 PE=3 SV=1	0.0	[123..553]	[91..506]
gi\|31982681, gi\|... - gi\|31982681\|ref\|NP_071877.2\| sirtuin 2 (silent mating type information regulation 2, homolog) 2 [Mus...	0.0	[178..553]	[11..361]
SIRT2 - sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) gi\|114677027 - gi\|114677027\|ref\|XP_001168375.1\| PREDICTED: sirtuin 2 isoform 8 [Pan troglodytes]	0.0	[178..553]	[11..361]

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Predicted Domain #1

Region A:
Residues: [1-191]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTIPHMKYAV SKTSENKVSN TVSPTQDKDA IRKQPDDIIN NDEPSHKKIK VAQPDSLRET  60
   61 NTTDPLGHTK AALGEVASME LKPTNDMDPL AVSAASVVSM SNDVLKPETP KGPIIISKNP 120
  121 SNGIFYGPSF TKRESLNARM FLKYYGAHKF LDTYLPEDLN SLYIYYLIKL LGFEVKDQAL 180
  181 IGTINSIVHI N

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [192-273]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SQERVQDLGS AISVTNVEDP LAKKQTVRLI KDLQRAINKV LCTRLRLSNF FTIDHFIQKL  60
   61 HTARKILVLT GAGVSTSLGI PD

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [317-366]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PPEKIYSPLH SFIKMLQMKG KLLRNYTQNI DNLESYAGIS TDKLVQCHGS

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [450-562]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PNKFHKSIRE DILECDLLIC IGTSLKVAPV SEIVNMVPSH VPQVLINRDP VKHAEFDLSL  60
   61 LGYCDDIAAM VAQKCGWTIP HKKWNDLKNK NFKCQEKDKG VYVVTSDEHP KTL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	480.38764
Match:	1j8fA_
Description:	Sirt2 histone deacetylase
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [274-316]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FRSSEGFYSK IKHLGLDDPQ DVFNYNIFMH DPSVFYNIAN MVL

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [367-449]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 FATATCVTCH WNLPGERIFN KIRNLELPLC PYCYKKRREY FPEGYNNKVG VAASQGSMSE  60
   61 RPPYILNSYG VLKPDITFFG EAL

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	480.38764
Match:	1j8fA_
Description:	Sirt2 histone deacetylase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
protein deacetylase activity	11.8771442637949	bayes_pls_golite062009
histone deacetylase activity	11.7308825525122	bayes_pls_golite062009
deacetylase activity	10.8368006972109	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	8.53375308475286	bayes_pls_golite062009
NAD-dependent protein deacetylase activity	8.13038616303736	bayes_pls_golite062009
NAD-dependent histone deacetylase activity	8.13038616303736	bayes_pls_golite062009
NAD-dependent histone deacetylase activity (H3-K9 specific)	7.45234027270448	bayes_pls_golite062009
NAD-dependent histone deacetylase activity (H3-K14 specific)	7.45234027270448	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	7.0185004322765	bayes_pls_golite062009
histone deacetylase activity (H3-K14 specific)	6.02509672218091	bayes_pls_golite062009
NAD-dependent histone deacetylase activity (H4-K16 specific)	5.11288441003953	bayes_pls_golite062009
tubulin deacetylase activity	4.98963898611597	bayes_pls_golite062009
histone deacetylase activity (H3-K9 specific)	4.84457718476539	bayes_pls_golite062009
NAD+ ADP-ribosyltransferase activity	4.62921662347995	bayes_pls_golite062009
transcription regulator activity	2.9155913558118	bayes_pls_golite062009
DNA binding	2.43141001866996	bayes_pls_golite062009
nucleic acid binding	2.41433477472968	bayes_pls_golite062009
transcription repressor activity	2.24657784428979	bayes_pls_golite062009
binding	2.06552694538163	bayes_pls_golite062009
NAD-independent histone deacetylase activity	2.03444106183496	bayes_pls_golite062009
histone binding	1.71445510915336	bayes_pls_golite062009
transcription corepressor activity	1.64212790017753	bayes_pls_golite062009
protein binding	1.52593340148652	bayes_pls_golite062009
nucleosome binding	1.5125174755597	bayes_pls_golite062009
hydrolase activity	1.43067365161346	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	1.40719303660557	bayes_pls_golite062009
transcription factor activity	1.30143289828088	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	1.26370407179255	bayes_pls_golite062009
transcription factor binding	1.14766678956419	bayes_pls_golite062009
NAD(P)+-protein-arginine ADP-ribosyltransferase activity	0.963519974868694	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.74741958699764	bayes_pls_golite062009
catalytic activity	0.739210399081789	bayes_pls_golite062009
cation transmembrane transporter activity	0.56434127203174	bayes_pls_golite062009
transcription cofactor activity	0.54005617175343	bayes_pls_golite062009
transferase activity	0.483523935618287	bayes_pls_golite062009

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: