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View Structure Prediction Details

Protein: NTG2
Organism: Saccharomyces cerevisiae
Length: 380 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for NTG2.

Description E-value Query
Range
Subject
Range
NTH2_YEAST - Endonuclease III homolog 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NTG2 PE=1 SV=...
NTG2 - DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase involved in base excision repair, localizes t...
gi|151945590 - gi|151945590|gb|EDN63831.1| endonuclease III DNA base excision repair N-glycosylase [Saccharomyces c...
2.0E-95 [1..380] [1..380]
gi|17380754, gi|... - gi|20259623|gb|AAM14168.1| putative endonuclease [Arabidopsis thaliana], gi|17380754|gb|AAL36207.1| ...
5.0E-76 [11..342] [38..361]
gi|229903773, gi... - gi|229903773|ref|ZP_04518886.1| adenine DNA glycosylase [Yersinia pestis Nepal516], gi|229679543|gb|...
gi|15979021, gi|... - gi|218928123|ref|YP_002345998.1| adenine DNA glycosylase [Yersinia pestis CO92], gi|16121255|ref|NP_...
gi|25292160 - pir||AH0116 A/G-specific adenine glycosylase (EC 3.2.2.-) [imported] - Yersinia pestis (strain CO92)
gi|149290654, gi... - gi|149367040|ref|ZP_01889073.1| A/G-specific adenine glycosylase [Yersinia pestis CA88-4125], gi|149...
gi|229837648, gi... - gi|229837648|ref|ZP_04457810.1| adenine DNA glycosylase [Yersinia pestis Pestoides A], gi|229704336|...
gi|229842567, gi... - gi|229842567|ref|ZP_04462722.1| adenine DNA glycosylase [Yersinia pestis biovar Orientalis str. Indi...
gi|229697238, gi... - gi|229840872|ref|ZP_04461031.1| adenine DNA glycosylase [Yersinia pestis biovar Orientalis str. PEXU...
8.0E-64 [130..379] [12..251]
gi|227008550, gi... - gi|227080662|ref|YP_002809213.1| A/G-specific adenine glycosylase [Vibrio cholerae M66-2], gi|227008...
gi|121728580, gi... - gi|121728580|ref|ZP_01681601.1| A/G-specific adenine glycosylase [Vibrio cholerae V52], gi|121629136...
gi|15640479, gi|... - gi|9654877|gb|AAF93625.1| A/G-specific adenine glycosylase [Vibrio cholerae O1 biovar El Tor str. N1...
gi|11354352 - pir||D82320 A/G-specific adenine glycosylase VC0452 [imported] - Vibrio cholerae (strain N16961 sero...
gi|125618186 - gi|125618186|gb|EAZ46730.1| A/G-specific adenine glycosylase [Vibrio cholerae MO10]
gi|153224226, gi... - gi|153224226|ref|ZP_01954309.1| A/G-specific adenine glycosylase [Vibrio cholerae MAK 757], gi|12411...
gi|153823186, gi... - gi|153823186|ref|ZP_01975853.1| A/G-specific adenine glycosylase [Vibrio cholerae B33], gi|126519312...
gi|153819148, gi... - gi|153819148|ref|ZP_01971815.1| A/G-specific adenine glycosylase [Vibrio cholerae NCTC 8457], gi|126...
3.0E-63 [127..379] [7..249]
MUTY_ECOLI - A/G-specific adenine glycosylase (EC 3.2.2.-) - Escherichia coli
EG10627 - adenine DNA glycosylase / Adenine DNA glycosylase, G-A mismatched DNA repair; 2-hydroxyadenine glyco...
gi|89109738, gi|... - gi|89109738|ref|AP_003518.1| adenine DNA glycosylase [Escherichia coli str. K-12 substr. W3110], gi|...
gi|169753726, gi... - gi|170018798|ref|YP_001723752.1| adenine DNA glycosylase [Escherichia coli ATCC 8739], gi|169753726|...
gi|75515259 - gi|75515259|ref|ZP_00737443.1| COG1194: A/G-specific DNA glycosylase [Escherichia coli 53638]
gi|169890348, gi... - gi|170082513|ref|YP_001731833.1| adenine DNA glycosylase [Escherichia coli str. K-12 substr. DH10B],...
gi|75196910 - gi|75196910|ref|ZP_00706980.1| COG1194: A/G-specific DNA glycosylase [Escherichia coli HS]
EG10627 - adenine DNA glycosylase / Adenine DNA glycosylase, G-A mismatched DNA repair; 2-hydroxyadenine glyco...
gi|124526139, gi... - gi|124526139|ref|ZP_01698144.1| A/G-specific adenine glycosylase [Escherichia coli B], gi|124502243|...
gi|238859929, gi... - gi|238902083|ref|YP_002927879.1| adenine DNA glycosylase [Escherichia coli BW2952], gi|238859929|gb|...
gi|242378487, gi... - gi|251786214|ref|YP_003000518.1| hypothetical protein B21_02754 [Escherichia coli BL21], gi|24237848...
gi|254289631, gi... - gi|254289631|ref|YP_003055379.1| adenine DNA glycosylase [Escherichia coli BL21(DE3)], gi|253978938|...
gi|253974772, gi... - gi|254162871|ref|YP_003045979.1| adenine DNA glycosylase [Escherichia coli B str. REL606], gi|253974...
3.0E-62 [130..379] [12..251]
gi|213609558 - gi|213609558|ref|ZP_03369384.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar...
gi|213425494 - gi|213425494|ref|ZP_03358244.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar...
gi|214000587 - gi|214000587|ref|ZP_03411322.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar...
gi|213850187 - gi|213850187|ref|ZP_03381085.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar...
gi|213646177 - gi|213646177|ref|ZP_03376230.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar...
gi|16761886 - gi|16761886|ref|NP_457503.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar Ty...
gi|25292161, gi|... - gi|25292161|pir||AG0879 A/G-specific adenine glycosylase STY3265 [imported] - Salmonella enterica su...
gi|29143373, gi|... - gi|29143373|ref|NP_806715.1| adenine DNA glycosylase [Salmonella enterica subsp. enterica serovar Ty...
1.0E-61 [130..379] [12..252]

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Predicted Domain #1
Region A:
Residues: [1-97]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MREESRSRKR KHIPVDIEEV EVRSKYFKKN ERTVELVKEN KINKDLQNYG GVNIDWIKAL  60
   61 KPIEYFEWIE SRTCDDPRTW GRPITKEEMI NDSGAKV

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below are all of our de novo (Rosetta) predictions for this domain.
Click here to view only most confident match.

    MCM
Score
SCOP
Match
SCOP Description
View Download 0.484 a.4.5 "Winged helix" DNA-binding domain
View Download 0.468 f.14.1 Voltage-gated potassium channels
View Download 0.343 a.60.9 lambda integrase-like, N-terminal domain
View Download 0.464 a.24.17 Group V grass pollen allergen
View Download 0.327 a.37.1 A DNA-binding domain in eukaryotic transcription factors
View Download 0.554 a.79.1 Antitermination factor NusB
View Download 0.537 c.58.1 Aminoacid dehydrogenase-like, N-terminal domain
View Download 0.463 a.45.1 Glutathione S-transferase (GST), C-terminal domain
View Download 0.433 d.110.3 PYP-like sensor domain (PAS domain)
View Download 0.408 a.1.1 Globin-like
View Download 0.322 a.60.2 RuvA domain 2-like
View Download 0.296 d.95.2 Homing endonucleases
View Download 0.284 a.177.1 Sigma2 domain of RNA polymerase sigma factors
View Download 0.280 d.54.1 Enolase N-terminal domain-like
View Download 0.271 d.88.1 SRF-like
View Download 0.252 d.38.1 Thioesterase/thiol ester dehydrase-isomerase
View Download 0.244 a.61.1 Retroviral matrix proteins
View Download 0.240 a.121.1 Tetracyclin repressor-like, C-terminal domain
View Download 0.236 a.28.1 ACP-like

Predicted Domain #2
Region A:
Residues: [98-140]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PESFLPIYNR VRLMRSKVKT PVDAMGCSMI PVLVSNKCGI PSE

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [250-380]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GYLTLQKGWG LIAGICVDVH VHRLCKMWNW VDPIKCKTAE HTRKELQVWL PHSLWYEINT  60
   61 VLVGFGQLIC MARGKRCDLC LANDVCNARN EKLIESSKFH QLEDKEDIEK VYSHWLDTVT 120
  121 NGITTERHKK K

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 170.927757
Match: 2abk__
Description: Endonuclease III
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
DNA N-glycosylase activity 4.8124964619976 bayes_pls_golite062009
hydrolase activity, hydrolyzing N-glycosyl compounds 4.26870112679392 bayes_pls_golite062009
DNA-(apurinic or apyrimidinic site) lyase activity 3.71152530486175 bayes_pls_golite062009
oxidized base lesion DNA N-glycosylase activity 2.49872706126491 bayes_pls_golite062009
hydrolase activity 2.39042189031819 bayes_pls_golite062009
hydrolase activity, acting on glycosyl bonds 2.24777884454113 bayes_pls_golite062009
catalytic activity 2.13345932727422 bayes_pls_golite062009
alkylbase DNA N-glycosylase activity 1.5832578018127 bayes_pls_golite062009
DNA binding 1.44394874535958 bayes_pls_golite062009
8-oxo-7,8-dihydroguanine DNA N-glycosylase activity 1.41538804527379 bayes_pls_golite062009
nucleic acid binding 1.36800347239439 bayes_pls_golite062009
damaged DNA binding 1.32391480975295 bayes_pls_golite062009
binding 1.15752946565805 bayes_pls_golite062009
DNA-3-methyladenine glycosylase I activity 1.06383943982125 bayes_pls_golite062009
oxidized pyrimidine base lesion DNA N-glycosylase activity 0.889914753262716 bayes_pls_golite062009
uracil DNA N-glycosylase activity 0.879508131574476 bayes_pls_golite062009
oxidized purine base lesion DNA N-glycosylase activity 0.789032002944782 bayes_pls_golite062009
purine-specific mismatch base pair DNA N-glycosylase activity 0.68578837518217 bayes_pls_golite062009
mismatch base pair DNA N-glycosylase activity 0.661651574677137 bayes_pls_golite062009
transcription regulator activity 0.493252955870142 bayes_pls_golite062009
structure-specific DNA binding 0.32094102946624 bayes_pls_golite062009
protein binding 0.0921713980805489 bayes_pls_golite062009
carbon-oxygen lyase activity 0.0676784802238829 bayes_pls_golite062009
double-stranded DNA binding 0.0420287478494503 bayes_pls_golite062009

Predicted Domain #3
Region A:
Residues: [141-249]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KVDPKNFRLQ FLIGTMLSAQ TRDERMAQAA LNITEYCLNT LKIAEGITLD GLLKIDEPVL  60
   61 ANLIRCVSFY TRKANFIKRT AQLLVDNFDS DIPYDIEGIL SLPGVGPKM

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 170.927757
Match: 2abk__
Description: Endonuclease III
Matching Structure (courtesy of the PDB):

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle