Protein Overview: DOT1

General Information:
Name(s) found:	DOT1 / YDR440W [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response [SGD]
Organism:	Saccharomyces cerevisiae
Length:	582 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	DNA damage checkpoint [IGI][IMP] recombinational repair [IGI][IMP] regulation of meiosis [IMP] histone methylation [IDA] nucleotide-excision repair [IGI][IMP] chromatin silencing at telomere [IMP] postreplication repair [IGI]
Molecular Function:	protein-lysine N-methyltransferase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGGQESISNN NSDSFIMSSP NLDSQESSIS PIDEKKGTDM QTKSLSSYSK GTLLSKQVQN  60
   61 LLEEANKYDP IYGSSLPRGF LRDRNTKGKD NGLVPLVEKV IPPIHKKTNN RNTRKKSSTT 120
  121 TKKDVKKPKA AKVKGKNGRT NHKHTPISKQ EIDTAREKKP LKKGRANKKN DRDSPSSTFV 180
  181 DWNGPCLRLQ YPLFDIEYLR SHEIYSGTPI QSISLRTNSP QPTSLTSDND TSSVTTAKLQ 240
  241 SILFSNYMEE YKVDFKRSTA IYNPMSEIGK LIEYSCLVFL PSPYAEQLKE TILPDLNASF 300
  301 DNSDTKGFVN AINLYNKMIR EIPRQRIIDH LETIDKIPRS FIHDFLHIVY TRSIHPQANK 360
  361 LKHYKAFSNY VYGELLPNFL SDVYQQCQLK KGDTFMDLGS GVGNCVVQAA LECGCALSFG 420
  421 CEIMDDASDL TILQYEELKK RCKLYGMRLN NVEFSLKKSF VDNNRVAELI PQCDVILVNN 480
  481 FLFDEDLNKK VEKILQTAKV GCKIISLKSL RSLTYQINFY NVENIFNRLK VQRYDLKEDS 540
  541 VSWTHSGGEY YISTVMEDVD ESLFSPAARG RRNRGTPVKY TR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		DBP8 DOT1 IMH1 TRM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	DOT1	RCL1	2	Unpublished Fields Lab Data
View Screen	DOT1	RCL1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DOT1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..174]	deduced	N/A	No confident structure predictions are available.
2	View Details	[175..327]	MSA	1.143996	View MSA. No confident structure predictions are available.
3	View Details	[328..391]	deduced	N/A	No confident structure predictions are available.
4	View Details	[392..511]	PSI-BLAST	680.211549	No description for 1m0rA_ was found.
5	View Details	[512..582]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
protein methyltransferase activity	5.03267617653315	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	4.4632173842967	bayes_pls_golite062009
histone methyltransferase activity	4.40911163698179	bayes_pls_golite062009
methyltransferase activity	4.15445027778357	bayes_pls_golite062009
N-methyltransferase activity	4.07806017452628	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	4.07153733729662	bayes_pls_golite062009
protein-arginine N-methyltransferase activity	3.26663548517117	bayes_pls_golite062009
arginine N-methyltransferase activity	3.26663548517117	bayes_pls_golite062009
histone-arginine N-methyltransferase activity	2.86617323549909	bayes_pls_golite062009
protein-arginine omega-N monomethyltransferase activity	2.48017990451667	bayes_pls_golite062009
histone methyltransferase activity (H4-R3 specific)	2.25250509433971	bayes_pls_golite062009
transferase activity	2.22303238506686	bayes_pls_golite062009
DNA-methyltransferase activity	2.18991764495147	bayes_pls_golite062009
transcription regulator activity	2.0187748912503	bayes_pls_golite062009
rRNA methyltransferase activity	1.93505048403302	bayes_pls_golite062009
DNA binding	1.86585662505513	bayes_pls_golite062009
nucleic acid binding	1.71901340148111	bayes_pls_golite062009
protein-arginine omega-N asymmetric methyltransferase activity	1.69391860928068	bayes_pls_golite062009
catalytic activity	1.25333584501255	bayes_pls_golite062009
binding	0.976078016260105	bayes_pls_golite062009
histone binding	0.964746527369069	bayes_pls_golite062009
protein-arginine omega-N symmetric methyltransferase activity	0.865238728135767	bayes_pls_golite062009
lysine N-methyltransferase activity	0.858129308056814	bayes_pls_golite062009
protein-lysine N-methyltransferase activity	0.858129308056814	bayes_pls_golite062009
histone-lysine N-methyltransferase activity	0.858129308056814	bayes_pls_golite062009
RNA methyltransferase activity	0.817509452491588	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	0.703178429468566	bayes_pls_golite062009
rRNA (adenine) methyltransferase activity	0.229189844833659	bayes_pls_golite062009
rRNA (adenine-N6,N6-)-dimethyltransferase activity	0.152064081957651	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2