Protein Overview: HDHA_ECOLI

General Information:
Name(s) found:	HDHA_ECOLI [Swiss-Prot]
Description(s) found: SHOW ONLY BEST	pir\|\|A38527 7alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) - Escherichia coli (strain K-12) [NCBI NR] gi\|912437\|dbj\|BAA01384.1\| 7alpha-hydroxysteroid dehydrogenase [Escherichia coli] [NCBI NR] gi\|84028835\|sp\|P0AET8.1\|HDHA_ECOLI RecName: Full=7-alpha-hydroxysteroid dehydrogenase; Short=7-alpha-HSDH [NCBI NR] pir\|\|G90919 7alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) - Escherichia coli (strain O157:H7, substrain RIMD 0509952) [NCBI NR] pir\|\|D85768 7alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) - Escherichia coli (strain O157:H7, substrain EDL933) [NCBI NR] gi\|209769766\|gb\|ACI83195.1\| NAD-dependent 7alpha-hydroxysteroid dehydrogenase [Escherichia coli] [NCBI NR] gi\|209769764\|gb\|ACI83194.1\| NAD-dependent 7alpha-hydroxysteroid dehydrogenase [Escherichia coli] [NCBI NR] gi\|209769762\|gb\|ACI83193.1\| NAD-dependent 7alpha-hydroxysteroid dehydrogenase [Escherichia coli] [NCBI NR] gi\|209769760\|gb\|ACI83192.1\| NAD-dependent 7alpha-hydroxysteroid dehydrogenase [Escherichia coli] [NCBI NR] gi\|209769758\|gb\|ACI83191.1\| NAD-dependent 7alpha-hydroxysteroid dehydrogenase [Escherichia coli] [NCBI NR] gi\|1943534\|pdb\|1FMC\|B Chain B, 7-Alpha-Hydroxysteroid Dehydrogenase Complex With Nadh And 7-Oxo Glycochenodeoxycholic Acid [NCBI NR] gi\|1943533\|pdb\|1FMC\|A Chain A, 7-Alpha-Hydroxysteroid Dehydrogenase Complex With Nadh And 7-Oxo Glycochenodeoxycholic Acid [NCBI NR] gi\|1827716\|pdb\|1AHH\|B Chain B, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nad+ [NCBI NR] gi\|1827715\|pdb\|1AHH\|A Chain A, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nad+ [NCBI NR] gi\|1827714\|pdb\|1AHI\|B Chain B, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nadh And 7-Oxo Glycochenodeoxycholic Acid [NCBI NR] gi\|1827713\|pdb\|1AHI\|A Chain A, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nadh And 7-Oxo Glycochenodeoxycholic Acid [NCBI NR] dbj\|BAA15407.1\| 7-a-hydroxysteroid dehydrogenase (EC 1.1.1.159) (7-alpha-HSDH). [Escherichia coli] [NCBI NR] dbj\|BAA15377.1\| 7-a-hydroxysteroid dehydrogenase (EC 1.1.1.159) (7-alpha-HSDH). [Escherichia coli] [NCBI NR] gi\|1742673\|dbj\|BAA15370.1\| 7alpha-hydroxysteroid dehydrogenase, NAD-dependent [Escherichia coli W3110] [NCBI NR] 7-alpha-hydroxysteroid dehydrogenase (EC 1.1.1.159) (7-alpha-HSDH) - Escherichia coli [Swiss-Prot] 7alpha-hydroxysteroid dehydrogenase, NAD-dependent; NAD-dependent 7alpha-hydroxysteroid dehydrogenase, dehydroxylation of bile acids [Escherichia coli K12]gi\|15831581\|ref\|NP_310354.1\| NAD-dependent 7alpha-hydroxysteroid dehydrogenase [Escherichia coli O1 [nrdb_11152004_Chlamydomonas] 7-alpha-hydroxysteroid dehydrogenase (7-alpha-HSDH) [NCBI-RefSeq_human_na_08-09-2006_reversed.fasta] Chain A, 7-Alpha-Hydroxysteroid Dehydrogenase Complex With Nadh And 7-Oxo Glycochenodeoxycholic Acid [nr-271106-contam.fasta] Chain B, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nad+ [nr-271106-contam.fasta] Chain B, 7-Alpha-Hydroxysteroid Dehydrogenase Complex With Nadh And 7-Oxo Glycochenodeoxycholic Acid [nr-271106-contam.fasta] Chain B, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nadh And 7-Oxo Glycochenodeoxycholic Acid [nr-271106-contam.fasta] Chain A, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nad+ [nr-271106-contam.fasta] Chain A, 7 Alpha-Hydroxysteroid Dehydrogenase Complexed With Nadh And 7-Oxo Glycochenodeoxycholic Acid [nr-271106-contam.fasta] 7-alpha-hydroxysteroid dehydrogenase OS=Escherichia coli (strain K12) GN=hdhA PE=1 SV=1 [uniprot-Ecoli-reviewed-110511.fasta]
Organism:	Escherichia coli
Length:	255 amino acids
Gene Ontology:
Cellular Component:	NONE FOUND
Biological Process:	NONE FOUND
Molecular Function:	NONE FOUND

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFNSDNLRLD GKCAIITGAG AGIGKEIAIT FATAGASVVV SDINADAANH VVDEIQQLGG  60
   61 QAFACRCDIT SEQELSALAD FAISKLGKVD ILVNNAGGGG PKPFDMPMAD FRRAYELNVF 120
  121 SFFHLSQLVA PEMEKNGGGV ILTITSMAAE NKNINMTSYA SSKAAASHLV RNMAFDLGEK 180
  181 NIRVNGIAPG AILTDALKSV ITPEIEQKML QHTPIRRLGQ PQDIANAALF LCSPAASWVS 240
  241 GQILTVSGGG VQELN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Noble WS, et al. (2009) Manuscript in preparation.		HDHA_ECOLI gi\|134105085, gi... gi\|189095939, gi... gi\|60594470, gi\|...

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

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Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
oxidoreductase activity	3.09625241707629	bayes_pls_golite062009
3-hydroxyacyl-CoA dehydrogenase activity	3.06391460989761	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	3.02758902218302	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	2.66329970371321	bayes_pls_golite062009
retinol dehydrogenase activity	2.50453100919665	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	1.81060491890041	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
steroid dehydrogenase activity	1.75109362865449	bayes_pls_golite062009
retinoid binding	1.72614743805853	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	1.65338499044318	bayes_pls_golite062009
isoprenoid binding	1.61095405873981	bayes_pls_golite062009
steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	1.55012034958204	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	1.47309297191111	bayes_pls_golite062009
fatty acid synthase activity	1.23679054027553	bayes_pls_golite062009
alcohol binding	1.07158175924364	bayes_pls_golite062009
sorbose reductase activity	1.06293597394863	bayes_pls_golite062009
binding	0.982999356179965	bayes_pls_golite062009
retinal dehydrogenase activity	0.69372321031969	bayes_pls_golite062009
3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity	0.665447800395781	bayes_pls_golite062009
3-oxoacyl-[acyl-carrier-protein] reductase activity	0.604742541607263	bayes_pls_golite062009
receptor activity	0.591732115418164	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.576163737662951	bayes_pls_golite062009
transmembrane receptor activity	0.565177620701637	bayes_pls_golite062009
testosterone 17-beta-dehydrogenase activity	0.532187027260366	bayes_pls_golite062009
DNA binding	0.525227236870065	bayes_pls_golite062009
nucleic acid binding	0.459537641913493	bayes_pls_golite062009
enoyl-CoA hydratase activity	0.439912268968251	bayes_pls_golite062009
signal transducer activity	0.384212208916324	bayes_pls_golite062009
molecular transducer activity	0.384212208916324	bayes_pls_golite062009
retinol binding	0.343379136183648	bayes_pls_golite062009
transcription regulator activity	0.177044638410302	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.0913823272876781	bayes_pls_golite062009
ethanol binding	0.0448614072391299	bayes_pls_golite062009
alcohol dehydrogenase (NAD) activity	0.00644214957417527	bayes_pls_golite062009

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.64

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..255]	PSI-BLAST	60.69897	7-alpha-hydroxysteroid dehydrogenase

Protein Overview: HDHA_ECOLI

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: