Protein Overview: pug-PB, pug-PD

General Information:
Name(s) found:	pug-PD / FBpp0099494 [FlyBase] pug-PB / FBpp0081741 [FlyBase]
Description(s) found: Found 33 descriptions. SHOW ALL	The gene pugilist is referred to in FlyBase by the symbol Dmel\pug (CG4067, FBgn0020385). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 3R:6522240..6529011. It has the cytological map location 86C4. Based on sequence similarity, it is predicted to have molecular function</span>: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process</span>: folic acid and derivative biosynthetic process. 16 alleles are reported. The phenotypes of these alleles are annotated with: eye; pigment cell. It has 4 annotated transcripts and 4 annotated polypeptides. [FlyBase] The gene pugilist is referred to in FlyBase by the symbol Dmel\pug (CG4067, FBgn0020385). It is a protein_coding_gene from Drosophila melanogaster. Its sequence location is 3R:6522240..6529011. It has the cytological map location 86C4. Based on sequence similarity, it is predicted to have molecular function</span>: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process</span>: folic acid and derivative biosynthetic process. 16 alleles are reported. The phenotypes of these alleles are annotated with: eye; pigment cell. It has 4 annotated transcripts and 4 annotated polypeptides. [FlyBase]
Organism:	Drosophila melanogaster
Length:	968 amino acids
Gene Ontology:
Cellular Component:	lipid particle [IDA]
Biological Process:	folic acid and derivative biosynthetic process [IEA]
Molecular Function:	ATP binding [IEA] formate-tetrahydrofolate ligase activity [ISS] methylenetetrahydrofolate dehydrogenase (NADP+) activity [ISS] methenyltetrahydrofolate cyclohydrolase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSAQYQRFLK VLEKWPAEKS KVGSGEWTAE PAIKMSGAKI ISGTAVAKSI REELRNEVTA  60
   61 MSKQLADFVP GLRIVQVGGR EDSNVYIRMK IKAATEIGID AAHVQLPRSI TEVELLDKIN 120
  121 DLNEDPRVHG IIVQMPLDCD TPIDSHRITD AVSPEKDVDG LHTVNEGRLA IGDLGGFLPC 180
  181 TPWGCLELIR RSGVEIAGAR AVVLGRSKIV GTPAAELLKW ANATVTVCHS KTRNLEEITR 240
  241 SADILVVGIG VAEMVKGSWI KPGAVVIDCG INVKPDASKA SGSKLVGDVD YAEALQVAGH 300
  301 LTPVPGGVGP MTVAMLMKNT VRSAARFLER LAKSQWALQT LPLKPQRPVP SDIVIARAQK 360
  361 PKDIAVLAKE IGLEAREVSL YGNKKAKISL SVLERLKDKE VGHYVVVAGM TPTPLGEGKT 420
  421 TTLMGLVQAL GAHKLRNTMA ALRQPSQGPT FGIKGGAAGG GYAQVIPMEE FNLHLTGDIH 480
  481 AVSAANNLLA AQLDTRIFHE NTQKDKALYD RLVPAIKGQR KFSPIQLRRL QKLGITKTDP 540
  541 DTLTADEYGP FARLDIDPDT IMWERVVDIN DRYLRTITVG QSPTEKGISR ETRFSISVAS 600
  601 EIMAVLALSR SLEDMKQRLA DMVVAFDKRG KPVTADDLGV TGALAVLLKD ALEPNLMQSL 660
  661 EGTPVLVHAG PFANIAHGCN SIIADEVGLK LVGKNGFVCT EAGFGSDIGM EKFCNIKCRT 720
  721 SGRKPNAMVL VATVRAIKMH GGGAPVTPGA PLNKQYTEEN LELVQKGLPN LLQHIENGKA 780
  781 FGMPVVVSLN AHSADTPAEH ELVKKAALEA GAFAAVVSTH WADGGAGAVQ LADAVIKACE 840
  841 QGNQFRLLYD LELPLVDKMN KIATTMYGAG KVVLSPAAEE KVKRLTDAGF GNLPICMSKV 900
  901 SGSFTGDAKI KGAPKGFTLD VEDVYVSAGA GFVVAMCGEV TKMPGLPTRP AIYDIDLNTE 960
  961 TGEIEGLF

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
translation initiation factor activity	2.41232228963167	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
lysine N-acetyltransferase activity	1.55478124277326	bayes_pls_golite062009
histone acetyltransferase activity	1.55478124277326	bayes_pls_golite062009
hydrolase activity	1.29060292615008	bayes_pls_golite062009
purine ribonucleotide binding	1.1110976601505	bayes_pls_golite062009
ribonucleotide binding	1.11103644113672	bayes_pls_golite062009
purine nucleotide binding	1.1086792521709	bayes_pls_golite062009
nucleotide binding	1.1060655022247	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.854899277460172	bayes_pls_golite062009
RNA helicase activity	0.81256037545135	bayes_pls_golite062009
ATP binding	0.80197288940364	bayes_pls_golite062009
adenyl ribonucleotide binding	0.770085840885632	bayes_pls_golite062009
adenyl nucleotide binding	0.751575230566049	bayes_pls_golite062009
RNA-dependent ATPase activity	0.74918137191219	bayes_pls_golite062009
structural constituent of ribosome	0.738234102557832	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.694120974355463	bayes_pls_golite062009
ATP-dependent RNA helicase activity	0.69117331267457	bayes_pls_golite062009
N-acyltransferase activity	0.581315571752634	bayes_pls_golite062009
N-acetyltransferase activity	0.487240078246257	bayes_pls_golite062009
kinase activity	0.431162005044036	bayes_pls_golite062009
ligase activity	0.417681194526243	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.347619818489809	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.250756824614616	bayes_pls_golite062009
acetyltransferase activity	0.190660928077915	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
nucleobase, nucleoside, nucleotide kinase activity	0.12351419478929	bayes_pls_golite062009
helicase activity	0.0775311459068899	bayes_pls_golite062009
structural molecule activity	0.0558641287316701	bayes_pls_golite062009
RNA binding	0.0261282189186349	bayes_pls_golite062009

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..342]	PSI-BLAST	108.0	Methylenetetrahydrofolate dehydrogenase/cyclohydrolase; Tetrahydrofolate dehydrogenase/cyclohydrolase
2	View Details	[343..968]	PSI-BLAST	1000.0	Formyltetrahydrofolate synthetase

Protein Overview: pug-PB, pug-PD

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: