Protein Overview: SPH1

General Information:
Name(s) found:	SPH1 / YLR313C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74644943\|sp\|Q06160.2\|SPH1_YEAST RecName: Full=Protein SPH1; AltName: Full=SPA2 homolog 1 [NCBI NR] gi\|42742288\|ref\|NP_013417.2\| Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste [NCBI NR] gi\|42544110\|gb\|AAB64516.2\| Ylr313cp [Saccharomyces cerevisiae] [NCBI NR] Protein SPH1 OS=Saccharomyces cerevisiae GN=SPH1 PE=1 SV=2 [Swiss-Prot] SPH1 Protein required for bipolar bud site selection [mips] Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p [SGD] SPH1 SGDID:S0004305, Chr XII from 762342-760357, reverse complement, Verified ORF [yeastflysequest.fasta] Protein involved in shmoo formation and required for bipolar bud site selection, homologous to Spa2p, localizes to sites of polarized growth [Saccharomyces cerevisiae]gi\|42544110\|gb\|AAB64516.2\| Ylr313cp [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] (Q06160) Ylr313cp [cerv4932.FASTAC] SPH1 SGDID:S000004305, Chr XII from 762342-760357, reverse complement, Verified ORF, "Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-depe [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein SPH1 (SPA2 homolog 1) [nr-271106-contam.fasta] Ylr313cp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p; Sph1p [Saccharomyces cere [nr-271106-contam.fasta] SPH1 SGDID:S000004305, Chr XII from 762342-760357, reverse complement, Verified ORF, "Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein SPH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPH1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
Organism:	Saccharomyces cerevisiae
Length:	661 amino acids
Gene Ontology:
Cellular Component:	polarisome [TAS] incipient cellular bud site [IDA] cellular bud tip [IDA ] cellular bud neck [IDA ] mating projection [IDA ]
Biological Process:	bipolar cellular bud site selection [TAS] pseudohyphal growth [IMP] actin filament organization [IPI] Rho protein signal transduction [IPI]
Molecular Function:	cytoskeletal regulatory protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MISSELTNDQ IIDLISDYKN FKRIIEASVP EDDRRRNQNR QNRNKDLTKL SNVQFWQLTT  60
   61 DVNDELMKRL TDSGADASLN DLDLKRGKAQ SKLSRLKDAK FHKLILDIFT EIERRNLHHL 120
  121 DMGTHNNGLD EGDLNFYLND TLFESFKIND DFMSVNGIIS IEVFRELKTQ FTLYFQNTLH 180
  181 RIDPVDTTTT RLPILLETII KIAKLIGDLL PVLSSVSLQS SLENEIVYLK SALSHAITST 240
  241 RYFLTYGDLI PRIVAQSSIS EVIFAFCNIA QIVKIKSTSR DDISRNEGEL SDIEAGMKPL 300
  301 KIIEKVKNEK NGKDISSLGD SGSTAVSFPS SGKPITKKSD MPVVVASPSI SIIEKSESSI 360
  361 RESGKVRNNT SGETNLASVS PLKNTKNSSR ITSEPSPREG LPLKVVSNSR SPSPQGNTLP 420
  421 LIGKFRQDYQ ASPPKKVITK PVAETAKPYA NIPPAADVLY SPTVTKMRKF REKVQKFAPN 480
  481 SGLGLRISTS EENLNNSDVN STTHNANINN LVEFVESKSM VVLPMAQGIL NDVQASKSKL 540
  541 FKSARSVSRL CQNSIEIIPI LESVIDMTTK AMVQKSFKLD LGEHCKEIIE RLTDCSQKLS 600
  601 ELCTYGCDST KLGKKRFYQK LADILMEVTK RTRELVECVK MANRQTLSQD LSSFFNYRSV 660
  661 Q

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		CLU1 CYC8 ECM10 SPH1 SSY5 TUP1
View Details	Qiu et al. (2008)		BNI1 BNR1 BUD6 HOF1 IQG1 MYO2 PEA2 SPA2 SPH1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RGT2	SPH1	2	Unpublished Fields Lab Data
View Screen	HMT1	SPH1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPH1	Sundin BA, et al. (2004)
View Data	SPH1	Sundin BA, et al. (2004)
View Data	SPH1	Sundin BA, et al. (2004)
View Data	SPH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..124]	MSA	9.01398	View MSA. No confident structure predictions are available.
2	View Details	[125..381]	deduced	N/A	No confident structure predictions are available.
3	View Details	[382..553]	deduced	N/A	No confident structure predictions are available.
4	View Details	[554..661]	MSA	1.002953	View MSA. Confident ab initio structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2