Protein Overview: uaf-1

General Information:
Name(s) found:	uaf-1 / CE27339 [WormBase]
Description(s) found: SHOW ONLY BEST	WBGene00006697 locus:uaf-1 Splicing factor U2AF 65 kDa subunit status:Confirmed UniProt:P90978 protein_id:CCD62278.1 [WormBase] gi\|71996475\|ref\|NP_001022967.1\| U2AF splicing factor family member (uaf-1) [Caenorhabditis elegans] [NCBI NR] gi\|6226906\|sp\|P90978.2\|U2AF2_CAEEL RecName: Full=Splicing factor U2AF 65 kDa subunit; AltName: Full=U2 auxiliary factor 65 kDa subunit; Short=U2AF65; AltName: Full=U2 snRNP auxiliary factor large subunit [NCBI NR] gi\|3334906\|gb\|AAC26982.1\| splicing factor U2AF65 [Caenorhabditis elegans] [NCBI NR] ref\|NP_497326.1\| U2AF splicing factor (55.4 kD) (uaf-1) [Caenorhabditis elegans] [NCBI NR] gi\|13559786\|gb\|AAK29989.1\| U2af splicing factor protein 1, isoform a [Caenorhabditis elegans] [NCBI NR] (P90978) Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa subunit) (U2 snRNP auxiliary factor large subunit) (U2AF65) [Swiss-Prot] Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans GN=uaf-1 PE=2 SV=2 [Swiss-Prot] [wormpep67.fasta] CE27339 WBGene00006697 locus:uaf-1 status:Confirmed SW:P90978 protein_id:AAK29989.1 [wormpep150.fasta] 27339 WBGene00006697 locus:uaf-1 status:Confirmed SW:P90978 protein_id:AAK29989.1 [wormpep130-phg-ecoli-orf-rand.fasta] U2AF splicing factor (55.4 kD) (uaf-1) [Caenorhabditis elegans]gi\|13559786\|gb\|AAK29989.1\| U2af splicing factor protein 1, isoform a [Caenorhabditis elegans]gi\|3334906\|gb\|AAC26982.1\| splicing factor U2AF65 [Caenorhabditis elegans]gi\|6226906\|sp\|P90978\|U2 [nrdb_11152004_Chlamydomonas] locus:uaf-1 status:Confirmed SW:P90978 protein_id:AAK29989.1 [wormpep115.fasta.reversed] CE27339 locus:uaf-1 status:Confirmed [wormpep80.hongbin] CE27339 WBGene00006697 locus:uaf-1 status:Confirmed UniProt:U2AF2_CAEEL protein_id:AAK29989.1 [WormBase_C-elegans_193_07-31-2008_con_reversed.fasta] CE27339 WBGene00006697 locus:uaf-1 status:Confirmed UniProt:U2AF2_CAEEL protein_id:AAK29989.1\| [wormORF-contam.fasta] U2AF splicing factor family member (uaf-1) [Caenorhabditis elegans] [nr-271106-contam.fasta] U2af splicing factor protein 1, isoform a [Caenorhabditis elegans] [nr-271106-contam.fasta] splicing factor U2AF65 [Caenorhabditis elegans] [nr-271106-contam.fasta] Splicing factor U2AF 65 kDa subunit (U2 auxiliary factor 65 kDa subunit) (U2 snRNP auxiliary factor large subunit) (U2AF65) [nr-271106-contam.fasta] [wormpep210-phg-orf-LaDeana-ecoli-contam.fasta.nr] CE27339 WBGene00006697 locus:uaf-1 status:Confirmed UniProt:P90978 protein_id:AAK29989.1 [wormbase_c-elegans_2.11_01-10-2010_reversed.fasta] CE27339 WBGene00006697 locus:uaf-1 status:Confirmed UniProt:P90978 protein_id:CCD62278.1 [wormbase_c_elegans_ws231_03-13-2012_reversed.fasta] CE27339 WBGene00006697 locus:uaf-1 status:Confirmed UniProt:P90978 protein_id:CCD62278.1 Splicing factor U2AF 65 kDa subunit [wormpep246-ecoli-contam.fasta]
Organism:	Caenorhabditis elegans
Length:	496 amino acids
Gene Ontology:
Cellular Component:	nucleus [IEA] spliceosomal complex [ISS]
Biological Process:	embryonic development [IMP] hermaphrodite genitalia development [IMP] nematode larval development [IMP] embryonic development ending in birth or egg hatching [IMP] mRNA processing [IEA] reproduction [IMP]
Molecular Function:	nucleic acid binding [IEA] RNA binding [IDA][IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDHQDGMKL EDIERQFLDV AQREGGLEAI QPTTGPLENE ENLKSSTGGG GGEDDNDRKK  60
   61 RKRSRSRDRD TRRRSRSRDR GERRGGGGGG DRDRSRSRER RRGGGGRDEP RRRGGDDEAR 120
  121 SRREPEPQKP REPKKYRFWD VPPTGFETTT PMEYKNMQAA GQVPRGSVQS AVPVVGPSVT 180
  181 CQSRRLYVGN IPFGCNEEAM LDFFNQQMHL CGLAQAPGNP ILLCQINLDK NFAFIEFRSI 240
  241 DETTAGMAFD GINFMGQQLK VRRPRDYQPS QNTFDMNSRM PVSTIVVDSA NKIFIGGLPN 300
  301 YLTEDQVKEL LCSFGPLKAF SLNVDSQGNS KGYAFAEYLD PTLTDQAIAG LNGMQLGDKQ 360
  361 LVVQLACANQ QRHNTNLPNS ASAIAGIDLS QGAGRATEIL CLMNMVTEDE LKADDEYEEI 420
  421 LEDVRDECSK YGIVRSLEIP RPYEDHPVPG VGKVFVEFAS TSDCQRAQAA LTGRKFANRT 480
  481 VVTSYYDVDK YHNRQF

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
RNA binding	6.73097473547076	bayes_pls_golite062009
	6.62953239642182	bayes_pls_golite062009
mRNA binding	5.29759606920196	bayes_pls_golite062009
nucleic acid binding	4.92126426323641	bayes_pls_golite062009
snRNA binding	4.06596327298973	bayes_pls_golite062009
binding	3.64400841800969	bayes_pls_golite062009
poly-pyrimidine tract binding	3.11990160533699	bayes_pls_golite062009
U1 snRNA binding	3.02511982045347	bayes_pls_golite062009
single-stranded RNA binding	2.93798024346416	bayes_pls_golite062009
transcription regulator activity	1.92450390013691	bayes_pls_golite062009
DNA binding	1.8021593476478	bayes_pls_golite062009
structural molecule activity	1.29928829870122	bayes_pls_golite062009
transcription factor activity	1.15127506309683	bayes_pls_golite062009
protein binding	1.12215421354121	bayes_pls_golite062009
mRNA 3'-UTR binding	0.591700155096213	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.425042944899091	bayes_pls_golite062009
transporter activity	0.417047632648771	bayes_pls_golite062009
sequence-specific DNA binding	0.383589644462932	bayes_pls_golite062009
cytoskeletal protein binding	0.288834004308736	bayes_pls_golite062009
transmembrane transporter activity	0.288804044686487	bayes_pls_golite062009
snoRNA binding	0.0568636182001701	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00171911393626201	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	6.73097473547076	bayes_pls_golite062009
	6.62953239642182	bayes_pls_golite062009
mRNA binding	5.29759606920196	bayes_pls_golite062009
nucleic acid binding	4.92126426323641	bayes_pls_golite062009
snRNA binding	4.06596327298973	bayes_pls_golite062009
binding	3.64400841800969	bayes_pls_golite062009
poly-pyrimidine tract binding	3.11990160533699	bayes_pls_golite062009
U1 snRNA binding	3.02511982045347	bayes_pls_golite062009
single-stranded RNA binding	2.93798024346416	bayes_pls_golite062009
transcription regulator activity	1.92450390013691	bayes_pls_golite062009
DNA binding	1.8021593476478	bayes_pls_golite062009
structural molecule activity	1.29928829870122	bayes_pls_golite062009
transcription factor activity	1.15127506309683	bayes_pls_golite062009
protein binding	1.12215421354121	bayes_pls_golite062009
mRNA 3'-UTR binding	0.591700155096213	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.425042944899091	bayes_pls_golite062009
transporter activity	0.417047632648771	bayes_pls_golite062009
sequence-specific DNA binding	0.383589644462932	bayes_pls_golite062009
cytoskeletal protein binding	0.288834004308736	bayes_pls_golite062009
transmembrane transporter activity	0.288804044686487	bayes_pls_golite062009
snoRNA binding	0.0568636182001701	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00171911393626201	bayes_pls_golite062009

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..157]	MSA	3.313996	View MSA. No confident structure predictions are available.
2	View Details	[158..289]	PSI-BLAST	34.69897	No description for 2g4bA was found.
3	View Details	[290..394]	PSI-BLAST	17.045757	Splicing factor U2AF 65 KDa subunit
4	View Details	[395..496]	PSI-BLAST	14.0	Splicing factor U2AF 65 KDa subunit

Protein Overview: uaf-1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: