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Protein Overview: eme1

General Information:
Name(s) found: eme1 / SPAPB1E7.06c [Sanger Pombe]
Description(s) found:
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  • gi|46395960|sp|Q9C103.1|EME1_SCHPO RecName: Full=Crossover junction endonuclease eme1; AltName: Full=Essential meiotic endonuclease 1 [NCBI NR]
  • gi|19115044|ref|NP_594132.1| Holliday junction resolvase subunit Eme1 [Schizosaccharomyces pombe 972h-] [NCBI NR]
  • gi|13624761|emb|CAC36923.1| Holliday junction resolvase subunit Eme1 [Schizosaccharomyces pombe] [NCBI NR]
  • Holliday junction resolvase subunit Eme1 [Sanger Pombe]
  • Crossover junction endonuclease eme1 OS=Schizosaccharomyces pombe GN=eme1 PE=1 SV=1 [Swiss-Prot]
  • HYPOTHETICAL PROTEIN; SEQUENCE ORPHAN; PREDICTED COILED-COIL REGION: Q9C103; [pompepVF.fasta]
  • HYPOTHETICAL PROTEIN; SEQUENCE ORPHAN; CONTAINS PUTATIVE COILED-COIL REGION: Q9C103; [pompepV13]
  • Hypothetical protein [pompep4896]
  • |eme1||DNA damage tolerance protein [pompepnew.fasta]
  • Essential meiotic endonuclease 1 (EC 3.1.22.4) (Crossover junction endonuclease eme1) (Holliday junction resolvase eme1) [4896.FASTAC]
  • |eme1||endodeoxyribonuclease RUS activity (PMID 11741546)|Schizosaccharomyces pombe|chr 1|||Manual [pombe041504.fasta]
  • hypothetical protein; sequence orphan; contains putative coiled-coil region [Schizosaccharomyces pombe]gi|13624761|emb|CAC36923.1| SPAPB1E7.06c [Schizosaccharomyces pombe]gi|46395960|sp|Q9C103|EME1_SCHPO Essential meiotic endonuclease 1 (Crossover junct [nrdb_11152004_Chlamydomonas]
  • |eme1||Holliday junction resolvase|Schizosaccharomyces pombe|chr 1|||Manual [pompep2242005.fasta]
  • (Q9C103) Hypothetical protein [pompep4896_normal_reversed]
  • |eme1|mms4, slx2|Holliday junction resolvase subunit Eme1|Schizosaccharomyces pombe|chr 1|||Manual [SGD_S-Pombe_na_10-23-2009_con_reversed.fasta]
  • SPAPB1E7.06c [Schizosaccharomyces pombe] [nr-271106-contam.fasta]
  • Crossover junction endonuclease eme1 (Essential meiotic endonuclease 1) [nr-271106-contam.fasta]
  • hypothetical protein SPAPB1E7.06c [Schizosaccharomyces pombe 972h-] [nr-271106-contam.fasta]
  • |eme1||Holliday junction resolvase subunit Eme1|Schizosaccharomyces pombe|chr 1|||Manual [spombe-190209-contam.fasta]
    • Organism: Schizosaccharomyces pombe
    Length: 738 amino acids

    Gene Ontology:
    Cellular Component: nucleus [ICLink to PubMed article]
    Holliday junction resolvase complex [TASLink to PubMed article]
    Biological Process: DNA repair [TASLink to PubMed article]
    meiotic joint molecule formation [TASLink to PubMed article]
    reciprocal meiotic recombination [IDALink to PubMed article]
    replication fork processing [IMPLink to PubMed article]
    intra-S DNA damage checkpoint [IDALink to PubMed article]
    Molecular Function: magnesium ion binding [IEA]
    DNA binding [IEA]
    crossover junction endodeoxyribonuclease activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTLQSTDSAI VIDSEADVDI SSSQASPSKV CRDNIALSEH NVITVLDTPQ RSTQCDSLLK  60
   61 SFSTPLVSGS EDVLPSPRDA LNITNKKSVT DNLLLSLTSS NQSTNTNLNP SSRVEIINLN 120
  121 SSPPNSLSSQ PKHQEFHLFH TPTIPRTTQL SSKTSSPIVI PDDNEQVASP LSKKAASLTS 180
  181 SPLKDFQSSP PLSTVLQKSH SLHDILLDTN DDDHFPFTQS PLTKTKSFND ALTSSSSILK 240
  241 PCMPSIASPT SNRLSHAPST PNLFPNQDSS NTIDLINNRS KTSVENQERT FNLTSDVHLD 300
  301 SPTSPSKHSS IEPNTSQEDS FQELPSLNKL SIQSRAFKKM RLPKISRTTD TPPASTSNSN 360
  361 KKNLDKLKKM RKLCSRSLEP YELDSNTQRK RKRYEDSLKK SKTLDKVDSL NRKMAKELDR 420
  421 KNSKELQKIN KVKRTKEECL SEIILLSPDD WASSWYSTVR SQLDSYNCQF VVNSNQPKDS 480
  481 IMWKRKVNNV FNSSTNRFEL SIEHEQIEPF ALLRLKCRDF IKYIEEDQAD TFFHEMSEKF 540
  541 KGCKLILLLE GIPNYFKSLK AELNRQYAAA VNSGTRPLLF GSLSKYQNFT KEKLESEIVR 600
  601 FSFEHSILIN TSNDEKETAQ WIVSFTGDIA LSRYKHFSKF SARASTTEIG HVKSADRIEN 660
  661 SLNFMLRQIL RVTPNIANAI CDQFDSIPSL IHHLKTHGEE SLTNVVIQSS ISERNLGPVL 720
  721 SRRIYNTFLC KEASSDAP

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..155] MSA 2.225996 View MSA. No confident structure predictions are available.
    2 View Details [156..372] MSA 8.244995 View MSA. No confident structure predictions are available.
    3 View Details [373..439] deduced N/A No confident structure predictions are available.
    4 View Details [440..738] FFAS03 2.22 XPF from Aeropyrum pernix, complex with DNA

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • four-way junction helicase activity
    		•  9.97778352333441 bayes_pls_golite062009
  • DNA helicase activity
    		•  7.39534793109438 bayes_pls_golite062009
  • endodeoxyribonuclease activity
    		•  3.3215200974849 bayes_pls_golite062009
  • single-stranded DNA specific endodeoxyribonuclease activity
    		•  3.18118446093396 bayes_pls_golite062009
  • deoxyribonuclease activity
    		•  3.15144163151183 bayes_pls_golite062009
  • excinuclease ABC activity
    		•  2.93145997831744 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  2.54099670773663 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  2.43123013915603 bayes_pls_golite062009
  • binding
    		•  2.15665005558542 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.99056241947033 bayes_pls_golite062009
  • helicase activity
    		•  1.95147423101621 bayes_pls_golite062009
  • damaged DNA binding
    		•  1.93355995630071 bayes_pls_golite062009
  • DNA binding
    		•  1.8937816608663 bayes_pls_golite062009
  • nuclease activity
    		•  1.53464565125134 bayes_pls_golite062009
  • endonuclease activity
    		•  1.2384221353329 bayes_pls_golite062009
  • pyrophosphatase activity
    		•  0.868370062080145 bayes_pls_golite062009
  • catalytic activity
    		•  0.776505703815067 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  0.757551245363434 bayes_pls_golite062009
  • protein binding
    		•  0.687937183662529 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  0.593379763919056 bayes_pls_golite062009
  • DNA strand annealing activity
    		•  0.591712648797139 bayes_pls_golite062009


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle