Protein Overview: MCM21

General Information:
Name(s) found:	MCM21 / YDR318W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|85690863\|sp\|Q06675.2\|MCM21_YEAST RecName: Full=Central kinetochore subunit MCM21; AltName: Full=Minichromosome maintenance protein 21; AltName: Full=Chromosome transmission fidelity protein 5 [NCBI NR] gi\|37362633\|ref\|NP_010604.2\| Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules; Mc [NCBI NR] Central kinetochore subunit MCM21 OS=Saccharomyces cerevisiae GN=MCM21 PE=1 SV=2 [Swiss-Prot] MCM21 Outer kinetochore protein [mips] Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules [SGD] Chr IV from 1103750-1103801,1103885-1104939 [yeastorf5.fasta] MCM21 SGDID:S0002726, Chr IV from 1103751-1103802,1103886-1104940, Verified ORF [yeastflysequest.fasta] MCM21 SGDID:S0002726, Chr IV from 1103753-1103804,1103888-1104942, Verified ORF [10112005cervSGD.fasta] Mcm21p [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] MCM21 SGDID:S000002726, Chr IV from 1103753-1103804,1103888-1104942, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits b [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MCM21 SGDID:S000002726, Chr IV from 1103755-1103806,1103890-1104944, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with cen [yeast-contam.fasta] MCM21 SGDID:S000002726, Chr IV from 1103756-1103807,1103891-1104945, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with cen [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Tax_Id=4932 Theoretical pI/Mw: 4.90 / 42971.00 COMA complex [ecoli-190209-contam-DG.fasta] Central kinetochore subunit MCM21 (Minichromosome maintenance protein 21) (Chromosome transmission fidelity protein 5) [nr-271106-contam.fasta] Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules; Mcm21p [Saccharomyces cerevisia [nr-271106-contam.fasta] MCM21 SGDID:S000002726, Chr IV from 1103756-1103807,1103891-1104945, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules" [yeast-bovrabprots.fasta] MCM21 SGDID:S000002726, Chr IV from 1103755-1103806,1103890-1104944, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules" [yeast-contam_custom.fasta] [chemostatdb.fasta] 368 bases [Ecoli-contam-Dam1-Mind-COMA-MBP-110.fasta] Central kinetochore subunit MCM21 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MCM21 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MCM21 SGDID:S000002726, Chr IV from 1103758-1103809,1103893-1104947, Genome Release 64-1-1, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules" [yeast-030211-contam.fasta] MCM21 SGDID:S000002726, Chr IV from 1103758-1103809,1103893-1104947, Genome Release 64-2-1, Verified ORF, "Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2" [20141208_orf_trans.fasta] TR7275\|c0_g1_i1\|g.5276 ORF TR7275\|c0_g1_i1\|g.5276 TR7275\|c0_g1_i1\|m.5276 type:complete len:369 (+) TR7275\|c0_g1_i1:74-1180(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDR318W SGDID:S000002726, Chr IV from 1103758-1103809,1103893-1104947, Genome Release 64-1-1, Verified ORF, "Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_160\|g.214 ORF ID=asmbl_160\|g.214 ID=asmbl_160\|m.214 type:complete len:369 (+) ID=asmbl_160:73-1179(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	368 amino acids
Gene Ontology:
Cellular Component:	COMA complex [IDA] condensed nuclear chromosome kinetochore [IDA]
Biological Process:	chromosome segregation [IPI]
Molecular Function:	protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSRIDDLQQD IESLLSEINS LEESREKLKA KIKDKRKNEE SANPIVQEFE DLFDQFPQLN  60
   61 NFLFNEHPEL EETDDKDISR AQADIPATPI PYEPKKRAKL ENEEILPEQE WVLKTQPMVQ 120
  121 HQMFDPGVAD LLDTDILTSP SKRKRKLKID DISTSDRSEL EDYIVLENVY RMFGITFFPL 180
  181 VDPIDLKIKD ASGEIFVDRE MLGIRLEVFS ERTSQFEKPH YVLLKKRIKS NSWFLFKHTI 240
  241 PSFIDVQGIF DDTNGGLVIS HDDAYLFAKR VFLQLVEVQK RRQIFKDLEA KKIIHDLDLD 300
  301 LESSMVSFFV KDIKVELFVK QNEIVSCSIL DDIHDFSQNN KSKWEIALLG SLDDLELKLN 360
  361 HSFATIFK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		AME1 CHL4 CTF19 CTF3 MCM21 MCM22 NKP1 NKP2 OKP1
View Details	(MIPS) Mewes HW, et al. (2004)		CTF19 MCM21 OKP1
View Details	Krogan NJ, et al. (2006)		AME1 ARG5,6 CHL4 CTF19 CTF3 IML3 MCM16 MCM21 MCM22 NKP1 NKP2 OKP1 PRP18 SET4 YNR062C
View Details	Gavin AC, et al. (2006)		CHL4 CTF19 CTF3 MCM21 MCM22 OKP1
View Details	Gavin AC, et al. (2002)		AME1 CHL4 CTF19 CTF3 IML3 MCM21 MCM22 NKP1
View Details	Qiu et al. (2008)		MCM16 MCM21 SPC25 TID3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NSE5	MCM21	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MCM21	Huh WK, et al. (2003)
View Data	MCM21 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..368]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2