Protein Overview: HMI1

General Information:
Name(s) found:	HMI1 / YOL095C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|841469\|gb\|AAC49064.1\| potential DNA helicase by amino acid sequence similarity [NCBI NR] gi\|6324477\|ref\|NP_014546.1\| Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD; Hmi1p [Saccharomy [NCBI NR] gi\|600463\|emb\|CAA58184.1\| orf 00920 [Saccharomyces cerevisiae] [NCBI NR] gi\|59799592\|sp\|Q12039.1\|HMI1_YEAST RecName: Full=ATP-dependent DNA helicase HMI1, mitochondrial; Flags: Precursor [NCBI NR] gi\|1419944\|emb\|CAA99107.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S57374 probable DNA helicase YOL095c - yeast (Saccharomyces cerevisiae) [NCBI NR] ATP-dependent DNA helicase HMI1, mitochondrial OS=Saccharomyces cerevisiae GN=HMI1 PE=1 SV=1 [Swiss-Prot] HMI1 Mitochondrial DNA helicase [mips] Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD [SGD] >Q12039 (Q12039) Chromosome XV reading frame ORF YOL095C [4932.FASTACCERV.txt] Chr XV from 141346-139226, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 139226-141346, reverse complement [yeastorf3.fasta] HMI1, Chr XV from 139226-141346, reverse complement [yeast_orfs2] HMI1 SGDID:S0005455, Chr XV from 141346-139226, reverse complement, Verified ORF [yeastflysequest.fasta] Hmi1p [Saccharomyces cerevisiae]gi\|600463\|emb\|CAA58184.1\| orf 00920 [Saccharomyces cerevisiae]gi\|1419944\|emb\|CAA99107.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|841469\|gb\|AAC49064.1\| potential DNA helicase by amino acid sequence similarity [nrdb_11152004_Chlamydomonas] (Q12039) ORF YOL095c (Similarity) (Orf 00920 protein) [cerv4932.FASTAC] HMI1 SGDID:S000005455, Chr XV from 141346-139226, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HMI1 SGDID:S000005455, Chr XV from 141347-139227, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; [yeast-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] orf 00920 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] potential DNA helicase by amino acid sequence similarity [nr-271106-contam.fasta] Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD; Hmi1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ATP-dependent DNA helicase HMI1, mitochondrial precursor [nr-271106-contam.fasta] HMI1 SGDID:S000005455, Chr XV from 141347-139227, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD" [yeast-bovrabprots.fasta] [chemostatdb.fasta] ATP-dependent DNA helicase HMI1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HMI1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HMI1 SGDID:S000005455, Chr XV from 141347-139227, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD" [yeast-030211-contam.fasta] HMI1 SGDID:S000005455, Chr XV from 141347-139227, Genome Release 64-2-1, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase; required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD" [20141208_orf_trans.fasta] YOL095C SGDID:S000005455, Chr XV from 141347-139227, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	706 amino acids
Gene Ontology:
Cellular Component:	mitochondrial matrix [IDA] mitochondrion [IDA]
Biological Process:	mitochondrial DNA metabolic process [IMP] mitochondrial genome maintenance [IMP]
Molecular Function:	ATP-dependent 3'-5' DNA helicase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDKLTPSQWK VINKSYEPAS TIKVIAGPGS GKTLTLLYKV LHLITVENIK PEEILIFSLT  60
   61 NKAVDSIIEN LLSIFENSHT NKEIVHQIGC YTVHGLANRI VVENEGMINI IEEIGWRGLM 120
  121 KLLPPSKRTP HHFRSYKELE KVVKDYKLNN AKNNNPVIEK LVELMDNCKV MTNDDLIIRA 180
  181 KKYLELDSSD SDASSFTQDL RNKYKVVLID EFQDLYPSLA PLITMICKGK QLIMFGDTNQ 240
  241 SIYGFLGSNN EIMSQLDNLH PKNSTTVLKL FDNFRSTPEI ISLASKIINR PLAEKQIIDD 300
  301 TDETPSELVR KLPSGVSPQI MTFDDLAAES EFIIDKITQL ICSSAKFSDI AILSRTNSHL 360
  361 TAIASILKKY GIPYQKLKSQ PDWMDDLRIQ FLLDILKVCS LASDEKHNRE FNTGDKWQSN 420
  421 FSILVTMSAL KGIGDASIQA LYKACSLKNL SIWKYLTMVP NFEWPLGLSI KKKMENYTSN 480
  481 LYEMIENDQV HQLDDPMELL EKVASITNNL NLNPTYFQSL SDAQSSLEFK THLQEMAQVM 540
  541 KVSKSNKPPG ISFVKWFLET YFDQTMVFHQ SQQALQTTGP GTVKLSTIHS AKGLEFPIVF 600
  601 LTNGSMSNFP MDTNALYVGI TRARNLLYMC NMKHERLVSK SSPYSRNIMS NNLFWTYYNK 660
  661 DLKRSVCDVK VTHGYNVQRY NQLRKNFGFY RAYSSLRGCK SVFRRI

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD1	HMI1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..274]	PSI-BLAST	362.0	DEXX box DNA helicase
2	View Details	[275..706]	PSI-BLAST	362.0	DEXX box DNA helicase

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2