Protein Overview: SEC12

General Information:
Name(s) found:	SEC12 / YNR026C [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER [SGD]
Organism:	Saccharomyces cerevisiae
Length:	471 amino acids
Gene Ontology:
Cellular Component:	integral to endoplasmic reticulum membrane [IDA] integral to membrane of membrane fraction [IDA]
Biological Process:	ER to Golgi vesicle-mediated transport [IDA] ER-associated protein catabolic process [IMP]
Molecular Function:	guanyl-nucleotide exchange factor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKFVTASYNV GYPAYGAKFL NNDTLLVAGG GGEGNNGIPN KLTVLRVDPT KDTEKEQFHI  60
   61 LSEFALEDND DSPTAIDASK GIILVGCNEN STKITQGKGN KHLRKFKYDK VNDQLEFLTS 120
  121 VDFDASTNAD DYTKLVYISR EGTVAAIASS KVPAIMRIID PSDLTEKFEI ETRGEVKDLH 180
  181 FSTDGKVVAY ITGSSLEVIS TVTGSCIARK TDFDKNWSLS KINFIADDTV LIAASLKKGK 240
  241 GIVLTKISIK SGNTSVLRSK QVTNRFKGIT SMDVDMKGEL AVLASNDNSI ALVKLKDLSM 300
  301 SKIFKQAHSF AITEVTISPD STYVASVSAA NTIHIIKLPL NYANYTSMKQ KISKFFTNFI 360
  361 LIVLLSYILQ FSYKHNLHSM LFNYAKDNFL TKRDTISSPY VVDEDLHQTT LFGNHGTKTS 420
  421 VPSVDSIKVH GVHETSSVNG TEVLCTESNI INTGGAEFEI TNATFREIDD A

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		SBH1 SEC12 SOP4 YOS1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC48	SEC12	1	Unpublished Fields Lab Data
View Screen	YHR122W	SEC12	1	Hazbun TR, et al. (2003)
View Screen	CTF13	SEC12	1	Unpublished Fields Lab Data
View Screen	RRB1	SEC12	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SEC12	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..337]	PSI-BLAST	68.154902	beta1-subunit of the signal-transducing G protein heterotrimer
2	View Details	[338..471]	PSI-BLAST	26.30103	Groucho/tle1, C-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
protein binding	1.61720914342874	bayes_pls_golite062009
binding	1.40353148474598	bayes_pls_golite062009
transporter activity	1.21549336905926	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.949459052802047	bayes_pls_golite062009
small conjugating protein ligase activity	0.876326238093745	bayes_pls_golite062009
substrate-specific transporter activity	0.734793983890004	bayes_pls_golite062009
protein transporter activity	0.304681243699511	bayes_pls_golite062009
nucleic acid binding	0.110806113669931	bayes_pls_golite062009
acid-amino acid ligase activity	0.0294947647335083	bayes_pls_golite062009
hydrolase activity	0.0078918747815433	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.51

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2