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Protein Overview: PUS4

General Information:
Name(s) found: PUS4 / YNL292W [SGD]
Description(s) found:
Found 26 descriptions.
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  • Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 403 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IMPLink to PubMed article]
    mitochondrion [IMPLink to PubMed article]
    Biological Process: tRNA modification [IDALink to PubMed article]
    Molecular Function: RNA binding [IEA]
    pseudouridine synthase activity [IDA][IEA][IGI]
    isomerase activity [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNGIFAIEKP SGITSNQFML KLQHALTKSQ VFSKEIQRAT AERKQQYEKQ TGKKASKRKL  60
   61 RKVSKVKMGH GGTLDPLASG VLVIGIGAGT KKLANYLSGT VKVYESEALF GVSTTSGDVE 120
  121 GEILSQNSVK HLNFDDLKTV EEKFVGQLKQ TPPIYAALKM DGKPLHEYAR EGKPLPRAIE 180
  181 PRQVTIYDLK VFSDSLKRDH DYPLLRPTTE EAVDTVKNLN ANMLNDVLYF SKEYTEKHGL 240
  241 DSEVAKVEEP FPLSEQEEQE IQKEGDSYRA PKLHFKANVS SGTYIRSLVS DIGKSMRSSC 300
  301 YMVKLIRLQQ QDWSLEKNNV FQLTDFTERD EKVWSKVLEK VLDEGATVDV IEELKKAEKE 360
  361 IPADVKECIV SSDQPGDEAT AETIETANAE EHSNTLKRKI EQV

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) DIA4 PUS4

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data PUS4 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..282] PSI-BLAST 267.30103 Pseudouridine synthase II TruB, C-terminal domain; Pseudouridine synthase II TruB
    2 View Details [283..403] PSI-BLAST 267.30103 Pseudouridine synthase II TruB, C-terminal domain; Pseudouridine synthase II TruB

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • pseudouridine synthase activity
    		•  5.19957135839682 bayes_pls_golite062009
  • intramolecular transferase activity
    		•  3.78688422405974 bayes_pls_golite062009
  • isomerase activity
    		•  2.91720316078381 bayes_pls_golite062009
  • catalytic activity
    		•  2.02180970970004 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.21173188039059 bayes_pls_golite062009
  • ligase activity
    		•  1.08945974848348 bayes_pls_golite062009
  • DNA binding
    		•  0.950713703876672 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.909704702595901 bayes_pls_golite062009
  • binding
    		•  0.730744558777463 bayes_pls_golite062009
  • RNA binding
    		•  0.706879359688019 bayes_pls_golite062009
  • transcription factor activity
    		•  0.459074630080185 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.83

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle