Protein Overview: MIH1

General Information:
Name(s) found:	MIH1 / YMR036C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323679\|ref\|NP_013750.1\| Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25; Mih1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1171007\|sp\|P23748.2\|MPIP_YEAST RecName: Full=M-phase inducer phosphatase; AltName: Full=Mitosis initiation protein MIH1; AltName: Full=Mitotic inducer homolog [NCBI NR] M-phase inducer phosphatase OS=Saccharomyces cerevisiae GN=MIH1 PE=1 SV=2 [Swiss-Prot] MIH1 M-phase inducing protein tyrosine phosphatase [mips] Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 [SGD] MIH1, Chr XIII from 341855-343519, reverse complement [yeast_orfs2] Chr XIII from 341855-343519, reverse complement [yeastorf3.fasta] Chr XIII from 343519-341855, reverse complement [yeastorf5.fasta] (P23748) M-phase inducer phosphatase (EC 3.1.3.48) (Mitosis initiation protein MIH1) (Mitotic inducer homolog) [4932.FASTACCERV.txt] MIH1 SGDID:S0004639, Chr XIII from 343519-341855, reverse complement, Verified ORF [yeastflysequest.fasta] Mih1p [Saccharomyces cerevisiae]gi\|1171007\|sp\|P23748\|MPIP_YEAST M-phase inducer phosphatase (Mitosis initiation protein MIH1) (Mitotic inducer homolog) [nrdb_11152004_Chlamydomonas] MIH1 SGDID:S000004639, Chr XIII from 343519-341855, reverse complement, Verified ORF, "Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] M-phase inducer phosphatase (Mitosis initiation protein MIH1) (Mitotic inducer homolog) [nr-271106-contam.fasta] Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25; Mih1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] M-phase inducer phosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MIH1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MIH1 SGDID:S000004639, Chr XIII from 343520-341856, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25" [yeast-030211-contam.fasta] MIH1 SGDID:S000004639, Chr XIII from 343520-341856, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25" [20141208_orf_trans.fasta] YMR036C SGDID:S000004639, Chr XIII from 343520-341856, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	554 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA]
Biological Process:	G2/M transition of mitotic cell cycle [IGI][IMP][ISS] regulation of cyclin-dependent protein kinase activity [IGI][IMP]
Molecular Function:	protein tyrosine phosphatase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS NVDDDLINKE  60
   61 NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE NDDIVLSMHF ASQTLQSPTR 120
  121 NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI 180
  181 PRSSRKSSQK FSNITQNTLN FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF 240
  241 FPRISPETLK NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK 300
  301 VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF YPDILILDGG 360
  361 YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES KRFATKNNSF RKLASPSNPN 420
  421 FFYRDSHQSS TTMASSALSF RFEPPPKLSL NHRRVSSGSS LNSSESTGDE NFFPILSKSS 480
  481 MSSNSNLSTS HMLLMDGLDT PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA 540
  541 RRSLFPSLET EDKK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		COR1 CPR1 HTB2 MIH1 QCR2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIH1	experimental1 - mih1-1	McCusker D, et al (2007)
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CLB5	MIH1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MIH1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..66]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[67..176]	MSA	3.031994	View MSA. No confident structure predictions are available.
3	View Details	[177..456]	PSI-BLAST	279.54902	CDC25b
4	View Details	[457..554]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2