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Protein Overview: PCK1

General Information:
Name(s) found: PCK1 / YKR097W [SGD]
Description(s) found:
Found 28 descriptions.
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  • Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 549 amino acids

    Gene Ontology:
    Cellular Component: cytosol [IDALink to PubMed article]
    Biological Process: gluconeogenesis [IEPLink to PubMed article]
    Molecular Function: phosphoenolpyruvate carboxykinase (ATP) activity [IDALink to PubMed article][IMPLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN KTVISSSGAL  60
   61 IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT WSINRERAAD YLRTRDHIYI 120
  121 VDAFAGWDPK YRIKVRVVCA RAYHALFMTN MLIRPTEEEL AHFGEPDFTV WNAGQFPANL 180
  181 HTQDMSSKST IEINFKAMEM IILGTEYAGE MKKGIFTVMF YLMPVHHNVL TLHSSANQGI 240
  241 QNGDVTLFFG LSGTGKTTLS ADPHRLLIGD DEHCWSDHGV FNIEGGCYAK CINLSAEKEP 300
  301 EIFDAIKFGS VLENVIYDEK SHVVDYDDSS ITENTRCAYP IDYIPSAKIP CLADSHPKNI 360
  361 ILLTCDASGV LPPVSKLTPE QVMYHFISGY TSKMAGTEQG VTEPEPTFSS CFGQPFLALH 420
  421 PIRYATMLAT KMSQHKANAY LINTGWTGSS YVSGGKRCPL KYTRAILDSI HDGSLANETY 480
  481 ETLPIFNLQV PTKVNGVPAE LLNPAKNWSQ GESKYRGAVT NLANLFVQNF KIYQDRATPD 540
  541 VLAAGPQFE

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..222]
    [284..344]    		 PSI-BLAST 2440.0 Phosphoenolpyruvate (PEP) carboxykinase (ATP-oxaloacetate carboxy-liase)
    2 View Details [223..283]
    [345..361]
    [391..438]    		 PSI-BLAST 2440.0 Phosphoenolpyruvate (PEP) carboxykinase (ATP-oxaloacetate carboxy-liase)
    3 View Details [362..390]
    [439..549]    		 PSI-BLAST 2440.0 Phosphoenolpyruvate (PEP) carboxykinase (ATP-oxaloacetate carboxy-liase)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • phosphoenolpyruvate carboxykinase activity
    		•  5.427657115096 bayes_pls_golite062009
  • phosphoenolpyruvate carboxykinase (ATP) activity
    		•  4.07297753409796 bayes_pls_golite062009
  • phosphoenolpyruvate carboxykinase (GTP) activity
    		•  3.22045404005498 bayes_pls_golite062009
  • catalytic activity
    		•  1.59551963655826 bayes_pls_golite062009
  • lyase activity
    		•  0.903636804009288 bayes_pls_golite062009
  • carbon-carbon lyase activity
    		•  0.63361553348301 bayes_pls_golite062009
  • carboxy-lyase activity
    		•  0.110170641189915 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle