YRC Public Data Repository - Protein Structure Prediction

Protein:	PCK1
Organism:	Saccharomyces cerevisiae
Length:	549 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for PCK1.

Description	E-value	Query Range	Subject Range
gi\|151941637 - gi\|151941637\|gb\|EDN60000.1\| phosphoenolpyruvate carboxylkinase [Saccharomyces cerevisiae YJM789] gi\|190409910 - gi\|190409910\|gb\|EDV13175.1\| phosphoenolpyruvate carboxylkinase [Saccharomyces cerevisiae RM11-1a] PCKA_YEAST - Phosphoenolpyruvate carboxykinase [ATP] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=... PCK1 - Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carboh...	0.0	[1..549]	[1..549]
PCKA_KLULA - Phosphoenolpyruvate carboxykinase [ATP] OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 7... PCKA_KLULA, PPCK... - Phosphoenolpyruvate carboxykinase [ATP] OS=Kluyveromyces lactis GN=PCK1 PE=3 SV=2, Phosphoenolpyruva...	0.0	[1..548]	[1..543]
gi\|13785471 - gi\|13785471\|dbj\|BAB43909.1\| phosphoenolpyruvate carboxykinase [Flaveria pringlei]	0.0	[27..548]	[132..658]
gi\|13785469 - gi\|13785469\|dbj\|BAB43908.1\| phosphoenolpyruvate carboxykinase [Flaveria trinervia]	0.0	[27..548]	[136..662]
PCKA_ARATH - Phosphoenolpyruvate carboxykinase [ATP] OS=Arabidopsis thaliana GN=PCKA PE=1 SV=1	0.0	[27..548]	[145..671]
gi\|16950587 - gi\|16950587\|gb\|AAG01894.2\| phosphoenolpyruvate carboxykinase [Lycopersicon esculentum]	0.0	[8..548]	[110..662]
gi\|19225036, gi\|... - gi\|37532252\|ref\|NP_920428.1\| putative phosphoenolpyruvate carboxykinase [Oryza sativa (japonica cult...	0.0	[29..548]	[113..636]

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Predicted Domain #1

Region A:
Residues: [1-222]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSPSKMNATV GSTSEVEQKI RQELALSDEV TTIRRNAPAA VLYEDGLKEN KTVISSSGAL  60
   61 IAYSGVKTGR SPKDKRIVEE PTSKDEIWWG PVNKPCSERT WSINRERAAD YLRTRDHIYI 120
  121 VDAFAGWDPK YRIKVRVVCA RAYHALFMTN MLIRPTEEEL AHFGEPDFTV WNAGQFPANL 180
  181 HTQDMSSKST IEINFKAMEM IILGTEYAGE MKKGIFTVMF YL

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [284-344]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EGGCYAKCIN LSAEKEPEIF DAIKFGSVLE NVIYDEKSHV VDYDDSSITE NTRCAYPIDY  60
   61 I

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	2440.0
Match:	1ii2A_
Description:	Phosphoenolpyruvate (PEP) carboxykinase (ATP-oxaloacetate carboxy-liase)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
phosphoenolpyruvate carboxykinase activity	5.427657115096	bayes_pls_golite062009
phosphoenolpyruvate carboxykinase (ATP) activity	4.07297753409796	bayes_pls_golite062009
phosphoenolpyruvate carboxykinase (GTP) activity	3.22045404005498	bayes_pls_golite062009
catalytic activity	1.59551963655826	bayes_pls_golite062009
lyase activity	0.903636804009288	bayes_pls_golite062009
carbon-carbon lyase activity	0.63361553348301	bayes_pls_golite062009
carboxy-lyase activity	0.110170641189915	bayes_pls_golite062009

Predicted Domain #2

Region A:
Residues: [223-283]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPVHHNVLTL HSSANQGIQN GDVTLFFGLS GTGKTTLSAD PHRLLIGDDE HCWSDHGVFN  60
   61 I

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [345-361]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PSAKIPCLAD SHPKNII

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [391-438]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 TSKMAGTEQG VTEPEPTFSS CFGQPFLALH PIRYATMLAT KMSQHKAN

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	2440.0
Match:	1ii2A_
Description:	Phosphoenolpyruvate (PEP) carboxykinase (ATP-oxaloacetate carboxy-liase)
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [362-390]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LLTCDASGVL PPVSKLTPEQ VMYHFISGY

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [439-549]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 AYLINTGWTG SSYVSGGKRC PLKYTRAILD SIHDGSLANE TYETLPIFNL QVPTKVNGVP  60
   61 AELLNPAKNW SQGESKYRGA VTNLANLFVQ NFKIYQDRAT PDVLAAGPQF E

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	2440.0
Match:	1ii2A_
Description:	Phosphoenolpyruvate (PEP) carboxykinase (ATP-oxaloacetate carboxy-liase)
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: