Protein Overview: NEO1

General Information:
Name(s) found:	NEO1 / YIL048W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|731806\|sp\|P40527.1\|ATC7_YEAST RecName: Full=Probable phospholipid-transporting ATPase NEO1 [NCBI NR] gi\|6322141\|ref\|NP_012216.1\| Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus; Neo1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S48431 probable membrane protein YIL048w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|557820\|emb\|CAA86174.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Probable phospholipid-transporting ATPase NEO1 OS=Saccharomyces cerevisiae GN=NEO1 PE=1 SV=1 [Swiss-Prot] NEO1 P-type ATPase, a proposed aminophospholipid translocase [mips] Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus [SGD] NEO1, Chr IX from 261436-264891 [yeast_orfs2] Chr IX from 261436-264891 [yeastorf3.fasta] (P40527) Potential phospholipid-transporting ATPase NEO1 (EC 3.6.3.1) [4932.FASTACCERV.txt] NEO1 SGDID:S0001310, Chr IX from 261436-264891, Verified ORF [yeastflysequest.fasta] ATPase that leads to neomycin-resistant protein when overexpressed; P-Type ATPase [Saccharomyces cerevisiae]gi\|557820\|emb\|CAA86174.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|731806\|sp\|P40527\|ATC7_YEAST Potential phospholipid-transporting AT [nrdb_11152004_Chlamydomonas] NEO1 SGDID:S000001310, Chr IX from 261436-264891, Verified ORF, "Protein involved in the retrograde transport from the Golgi complex to the ER and the endosomal membrane traffic; putative aminophospholipid translocase; member of the highly conserved Drs2 [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NEO1 SGDID:S000001310, Chr IX from 261436-264891, Verified ORF, "Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Probable phospholipid-transporting ATPase NEO1 [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein involved in the retrograde transport from the Golgi complex to the ER and the endosomal membrane traffic; putative aminophospholipid translocase; member of the highly conserved Drs2 family of P-type ATPases; Neo1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NEO1 SGDID:S000001310, Chr IX from 261436-264891, Verified ORF, "Protein involved in the retrograde transport from the Golgi complex to the ER and the endosomal membrane traffic; putative aminophospholipid translocase; member of the highly conserved Drs2 family of P-type ATPases" [yeast-contam_custom.fasta] [chemostatdb.fasta] Probable phospholipid-transporting ATPase NEO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NEO1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NEO1 SGDID:S000001310, Chr IX from 261437-264892, Genome Release 64-1-1, Verified ORF, "Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus" [yeast-030211-contam.fasta] NEO1 SGDID:S000001310, Chr IX from 261437-264892, Genome Release 64-2-1, Verified ORF, "Putative aminophospholipid translocase (flippase); involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus" [20141208_orf_trans.fasta] TR1907\|c0_g1_i1\|g.1382 ORF TR1907\|c0_g1_i1\|g.1382 TR1907\|c0_g1_i1\|m.1382 type:complete len:1152 (+) TR1907\|c0_g1_i1:308-3763(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6453\|c0_g1_i1\|g.4638 ORF TR6453\|c0_g1_i1\|g.4638 TR6453\|c0_g1_i1\|m.4638 type:complete len:1152 (+) TR6453\|c0_g1_i1:393-3848(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YIL048W SGDID:S000001310, Chr IX from 261437-264892, Genome Release 64-1-1, Verified ORF, "Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_8785\|g.6425 ORF asmbl_8785\|g.6425 asmbl_8785\|m.6425 type:complete len:1152 (+) asmbl_8785:393-3848(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_8929\|g.6424 ORF asmbl_8929\|g.6424 asmbl_8929\|m.6424 type:complete len:1152 (+) asmbl_8929:306-3761(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_8758\|g.5478 ORF ID=asmbl_8758\|g.5478 ID=asmbl_8758\|m.5478 type:complete len:1152 (+) ID=asmbl_8758:393-3848(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_8935\|g.5379 ORF ID=asmbl_8935\|g.5379 ID=asmbl_8935\|m.5379 type:complete len:1152 (+) ID=asmbl_8935:306-3761(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1151 amino acids
Gene Ontology:
Cellular Component:	endosome [IDA] Golgi membrane [IDA] Golgi apparatus [IDA] COPI-coated vesicle [IDA]
Biological Process:	retrograde vesicle-mediated transport, Golgi to ER [IMP] vacuole organization [IMP] endocytosis [IMP]
Molecular Function:	ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism [ISS] phospholipid-translocating ATPase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPNPPSFKSH KQNLFNSNNN QHANSVDSFD LHLDDSFDAA LDSLQINNNP EPLSKHNTVG  60
   61 DRESFEMRTV DDLDNFSNHS SDSHRKSSNT DTHPLMYDNR LSQDDNFKFT NIASSPPSSS 120
  121 NNIFSKALSY LKVSNTKNWS KFGSPIELSD QHIEREIHPD TTPVYDRNRY VSNELSNAKY 180
  181 NAVTFVPTLL YEQFKFFYNL YFLVVALSQA VPALRIGYLS SYIVPLAFVL TVTMAKEAID 240
  241 DIQRRRRDRE SNNELYHVIT RNRSIPSKDL KVGDLIKVHK GDRIPADLVL LQSSEPSGES 300
  301 FIKTDQLDGE TDWKLRVACP LTQNLSENDL INRISITASA PEKSIHKFLG KVTYKDSTSN 360
  361 PLSVDNTLWA NTVLASSGFC IACVVYTGRD TRQAMNTTTA KVKTGLLELE INSISKILCA 420
  421 CVFALSILLV AFAGFHNDDW YIDILRYLIL FSTIIPVSLR VNLDLAKSVY AHQIEHDKTI 480
  481 PETIVRTSTI PEDLGRIEYL LSDKTGTLTQ NDMQLKKIHL GTVSYTSETL DIVSDYVQSL 540
  541 VSSKNDSLNN SKVALSTTRK DMSFRVRDMI LTLAICHNVT PTFEDDELTY QAASPDEIAI 600
  601 VKFTESVGLS LFKRDRHSIS LLHEHSGKTL NYEILQVFPF NSDSKRMGII VRDEQLDEYW 660
  661 FMQKGADTVM SKIVESNDWL EEETGNMARE GLRTLVIGRK KLNKKIYEQF QKEYNDASLS 720
  721 MLNRDQQMSQ VITKYLEHDL ELLGLTGVED KLQKDVKSSI ELLRNAGIKI WMLTGDKVET 780
  781 ARCVSISAKL ISRGQYVHTI TKVTRPEGAF NQLEYLKINR NACLLIDGES LGMFLKHYEQ 840
  841 EFFDVVVHLP TVIACRCTPQ QKADVALVIR KMTGKRVCCI GDGGNDVSMI QCADVGVGIV 900
  901 GKEGKQASLA ADFSITQFCH LTELLLWHGR NSYKRSAKLA QFVMHRGLII AICQAVYSIC 960
  961 SLFEPIALYQ GWLMVGYATC YTMAPVFSLT LDHDIEESLT KIYPELYKEL TEGKSLSYKT1020
 1021 FFVWVLLSLF QGSVIQLFSQ AFTSLLDTDF TRMVAISFTA LVVNELIMVA LEIYTWNKTM1080
 1081 LVTEIATLLF YIVSVPFLGD YFDLGYMTTV NYYAGLLVIL LISIFPVWTA KAIYRRLHPP1140
 1141 SYAKVQEFAT P

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		DNF3 DRS2 NEO1 PMR1 YOS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NUC1	NEO1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NEO1	Huh WK, et al. (2003)
View Data	NEO1 and COP1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..114]	deduced	N/A	No confident structure predictions are available.
2	View Details	[115..177] [254..356]	PSI-BLAST	1072.218487	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M
3	View Details	[178..246] [357..466] [932..1151]	PSI-BLAST	1072.218487	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M
4	View Details	[247..253] [467..510] [752..931]	PSI-BLAST	1072.218487	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M
5	View Details	[511..751]	PSI-BLAST	1072.218487	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2