Protein Overview: XKS1

General Information:
Name(s) found:	XKS1 / YGR194C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321633\|ref\|NP_011710.1\| Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains; Xks1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S64512 probable membrane protein YGR194c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1723736\|sp\|P42826.2\|XKS1_YEAST RecName: Full=Xylulose kinase; Short=Xylulokinase [NCBI NR] gi\|1323345\|emb\|CAA97220.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Xylulose kinase OS=Saccharomyces cerevisiae GN=XKS1 PE=1 SV=2 [Swiss-Prot] XKS1 Xylulokinase [mips] Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains [SGD] XKS1, Chr VII from 886068-887870, reverse complement [yeast_orfs2] Chr VII from 886068-887870, reverse complement [yeastorf3.fasta] Chr VII from 886075-887877, reverse complement [yeastorf4.fasta] Chr VII from 887877-886075, reverse complement [yeastorf5.fasta] (P42826) Xylulose kinase (EC 2.7.1.17) (Xylulokinase) [4932.FASTACCERV.txt] XKS1 SGDID:S0003426, Chr VII from 887878-886076, reverse complement, Verified ORF [yeastflysequest.fasta] XKS1 SGDID:S0003426, Chr VII from 887880-886078, reverse complement, Verified ORF [10112005cervSGD.fasta] Xks1p [Saccharomyces cerevisiae]gi\|1323345\|emb\|CAA97220.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2132623\|pir\|\|S64512 probable membrane protein YGR194c - yeast (Saccharomyces cerevisiae)gi\|1723736\|sp\|P42826\|XKS1_YEAST Xylulose kinase (Xyl [nrdb_11152004_Chlamydomonas] XKS1 SGDID:S000003426, Chr VII from 887880-886078, reverse complement, Verified ORF, "Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinan [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Xylulose kinase (Xylulokinase) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains; Xks1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] XKS1 SGDID:S000003426, Chr VII from 887880-886078, reverse complement, Verified ORF, "Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Xylulose kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=XKS1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] XKS1 SGDID:S000003426, Chr VII from 887875-886073, Genome Release 64-1-1, reverse complement, Verified ORF, "Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains" [yeast-030211-contam.fasta] XKS1 SGDID:S000003426, Chr VII from 887875-886073, Genome Release 64-2-1, reverse complement, Verified ORF, "Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains" [20141208_orf_trans.fasta] YGR194C SGDID:S000003426, Chr VII from 887875-886073, Genome Release 64-1-1, reverse complement, Verified ORF, "Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	600 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	xylulose catabolic process [IDA]
Molecular Function:	xylulokinase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLCSVIQRQT REVSNTMSLD SYYLGFDLST QQLKCLAINQ DLKIVHSETV EFEKDLPHYH  60
   61 TKKGVYIHGD TIECPVAMWL EALDLVLSKY REAKFPLNKV MAVSGSCQQH GSVYWSSQAE 120
  121 SLLEQLNKKP EKDLLHYVSS VAFARQTAPN WQDHSTAKQC QEFEECIGGP EKMAQLTGSR 180
  181 AHFRFTGPQI LKIAQLEPEA YEKTKTISLV SNFLTSILVG HLVELEEADA CGMNLYDIRE 240
  241 RKFSDELLHL IDSSSKDKTI RQKLMRAPMK NLIAGTICKY FIEKYGFNTN CKVSPMTGDN 300
  301 LATICSLPLR KNDVLVSLGT STTVLLVTDK YHPSPNYHLF IHPTLPNHYM GMICYCNGSL 360
  361 ARERIRDELN KERENNYEKT NDWTLFNQAV LDDSESSENE LGVYFPLGEI VPSVKAINKR 420
  421 VIFNPKTGMI EREVAKFKDK RHDAKNIVES QALSCRVRIS PLLSDSNASS QQRLNEDTIV 480
  481 KFDYDESPLR DYLNKRPERT FFVGGASKND AIVKKFAQVI GATKGNFRLE TPNSCALGGC 540
  541 YKAMWSLLYD SNKIAVPFDK FLNDNFPWHV MESISDVDNE NWDRYNSKIV PLSELEKTLI 600
  601

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		PRO1 XKS1
View Details	Gavin AC, et al. (2002)		CCT4 CHC1 COG8 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 GUT1 IST1 PDC1 PRO1 RRP40 RRP46 SUI2 SUI3 XKS1
View Details	Qiu et al. (2008)		GLK1 XKS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBC7	XKS1	1	Unpublished Fields Lab Data
View Screen	HOF1	XKS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	XKS1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..297]	PSI-BLAST	141.0	Glycerol kinase
2	View Details	[298..352] [532..560]	PSI-BLAST	141.0	Glycerol kinase
3	View Details	[353..531] [561..600]	PSI-BLAST	141.0	Glycerol kinase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
carbohydrate kinase activity	2.33338276303563	bayes_pls_golite062009
hexokinase activity	1.70516840340504	bayes_pls_golite062009
binding	1.44744777288539	bayes_pls_golite062009
glucokinase activity	0.47468456642723	bayes_pls_golite062009
kinase activity	0.345540463799773	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.324655711623803	bayes_pls_golite062009
catalytic activity	0.296484721581333	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.214617425075414	bayes_pls_golite062009
protein binding	0.213044068887966	bayes_pls_golite062009
nucleic acid binding	0.132883501060045	bayes_pls_golite062009
transferase activity	0.105619024604158	bayes_pls_golite062009
glucose binding	0.0361890405625098	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2