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Protein Overview: MAD1

General Information:
Name(s) found: MAD1 / YGL086W [SGD]
Description(s) found:
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  • gi|729975|sp|P40957.1|MAD1_YEAST RecName: Full=Spindle assembly checkpoint component MAD1; AltName: Full=Mitotic arrest deficient protein 1 [NCBI NR]
  • gi|6321352|ref|NP_011429.1| Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p; Mad1p [NCBI NR]
  • gi|551092|gb|AAA91620.1| Mad1p [NCBI NR]
  • pir||A57276 MAD1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1322611|emb|CAA96791.1| MAD1 [Saccharomyces cerevisiae] [NCBI NR]
  • Spindle assembly checkpoint component MAD1 OS=Saccharomyces cerevisiae GN=MAD1 PE=1 SV=1 [Swiss-Prot]
  • MAD1 Spindle assembly checkpoint protein [mips]
  • Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p [SGD]
  • MAD1, Chr VII from 347117-349366 [yeast_orfs2]
  • Chr VII from 347117-349366 [yeastorf3.fasta]
  • Chr VII from 347120-349369 [yeastorf4.fasta]
  • (P40957) Spindle assembly checkpoint component MAD1 (Mitotic MAD1 protein) [4932.FASTACCERV.txt]
  • MAD1 SGDID:S0003054, Chr VII from 347120-349369, Verified ORF [yeastflysequest.fasta]
  • MAD1 SGDID:S0003054, Chr VII from 347122-349371, Verified ORF [10112005cervSGD.fasta]
  • Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p [Saccharomyces cerevisiae]gi|13226 [nrdb_11152004_Chlamydomonas]
  • (P40957) Spindle assembly checkpoint component MAD1 (Mitotic arrest deficient protein 1) [cerv4932.FASTAC]
  • MAD1 SGDID:S000003054, Chr VII from 347122-349371, Verified ORF, "Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting compl [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p; Mad1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • MAD1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Spindle assembly checkpoint component MAD1 (Mitotic arrest deficient protein 1) [nr-271106-contam.fasta]
  • Mad1p [nr-271106-contam.fasta]
  • MAD1 SGDID:S000003054, Chr VII from 347122-349371, Verified ORF, "Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Spindle assembly checkpoint component MAD1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MAD1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • MAD1 SGDID:S000003054, Chr VII from 347119-349368, Genome Release 64-1-1, Verified ORF, "Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p" [yeast-030211-contam.fasta]
  • MAD1 SGDID:S000003054, Chr VII from 347119-349368, Genome Release 64-2-1, Verified ORF, "Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability" [20141208_orf_trans.fasta]
  • YGL086W SGDID:S000003054, Chr VII from 347119-349368, Genome Release 64-1-1, Verified ORF, "Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 749 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    nuclear pore [IDALink to PubMed article]
    Biological Process: mitotic cell cycle spindle assembly checkpoint [IGILink to PubMed article][IMPLink to PubMed article]
    nucleocytoplasmic transport [IGILink to PubMed article][IPILink to PubMed article]
    Molecular Function: molecular_function [ND]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDVRAALQCF FSALSGRFTG KKLGLEIYSI QYKMSNSGGS SPFLESPGGS PDVGSTNGQS  60
   61 NRQIQALQFK LNTLQNEYEI EKLQLQKQTN ILEKKYKATI DELEKALNDT KYLYESNDKL 120
  121 EQELKSLKER SANSMNDKDK CIEELRTTLQ NKDLEMETLR QQYDSKLSKV TNQCDHFKLE 180
  181 AESSHSLLMK YEKEIKRQSV DIKDLQHQVM EKDDELSSVK ASKMINSHPN YSTEEFNELT 240
  241 EMNKMIQDQV QYTKELELAN MQQANELKKL KQSQDTSTFW KLENEKLQNK LSQLHVLESQ 300
  301 YENLQLENID LKSKLTKWEI YNDSDDDDDN NVNNNDNNNN NKNDNNNDNN NDTSNNNNIN 360
  361 NNNRTKNNIR NNPEEIIRDW KLTKKECLIL TDMNDKLRLD NNNLKLLNDE MALERNQILD 420
  421 LNKNYENNIV NLKRLNHELE QQKSLSFEEC RLLREQLDGL YSAQNNALLE VENSETHASN 480
  481 KNVNEDMNNL IDTYKNKTED LTNELKKLND QLLSNSNDVE TQRKKRKLTS DQIGLNYSQR 540
  541 LNELQLENVS VSRELSKAQT TIQLLQEKLE KLTKLKEKKI RILQLRDGPF IKDQFIKKNK 600
  601 LLLLEKENAD LLNELKKNNP AVETVPISVY DSLNFELKQF EQEVFKSNKR FSRLKQVFNN 660
  661 KSLEFIDVVN SLLGFKLEFQ QDSRVKIFSC FKPEKYLIAD LNENTLKSNL DADIEGWDDL 720
  721 MNLWVEDRGQ LPCFLATITL RLWEQRQAK

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) MAD1 MAD2
    View Details Krogan NJ, et al. (2006) MAD1 MAD2
    View Details Qiu et al. (2008) MAD1 MLP1 MLP2 SUS1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #04 Alpha Factor Prep2 Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen NUP2 MAD1 1 Unpublished Fields Lab Data
    View Screen ECM29 MAD1 1 Unpublished Fields Lab Data
    View Screen SPT23 MAD1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data MAD1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..275] PSI-BLAST 16.154902 Heavy meromyosin subfragment
    2 View Details [276..364] PSI-BLAST 11.69897 Tropomyosin
    3 View Details [365..446] PSI-BLAST 11.69897 Tropomyosin
    4 View Details [447..508] PSI-BLAST 11.69897 Tropomyosin
    5 View Details [509..619] PSI-BLAST 11.69897 Tropomyosin
    6 View Details [620..749] MSA 3.855997 View MSA. Confident ab initio structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.59

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle