Protein Overview: ALG13

General Information:
Name(s) found:	ALG13 / YGL047W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321391\|ref\|NP_011468.1\| Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransfera [NCBI NR] pir\|\|S64051 hypothetical protein YGL047w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1723827\|sp\|P53178.1\|ALG13_YEAST RecName: Full=UDP-N-acetylglucosamine transferase subunit ALG13; AltName: Full=Asparagine-linked glycosylation protein 13 [NCBI NR] gi\|1322537\|emb\|CAA96749.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (P53178) Hypothetical glycosyl transferase YGL074W (EC 2.-.-.-) [Swiss-Prot] UDP-N-acetylglucosamine transferase subunit ALG13 OS=Saccharomyces cerevisiae GN=ALG13 PE=1 SV=1 [Swiss-Prot] ALG13 Essential protein required for the second step of dolichyl-linked oligosaccharide synthesis [mips] Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases [SGD] YGL047W, Chr VII from 411549-412157 [yeast_orfs2] Chr VII from 411549-412157 [yeastorf3.fasta] Chr VII from 411553-412161 [yeastorf4.fasta] YGL047W SGDID:S0003015, Chr VII from 411553-412161, Uncharacterized ORF [yeastflysequest.fasta] YGL047W SGDID:S0003015, Chr VII from 411555-412163, Uncharacterized ORF [10112005cervSGD.fasta] Protein required for cell viability [Saccharomyces cerevisiae]gi\|1322537\|emb\|CAA96749.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1723827\|sp\|P53178\|YGE7_YEAST Hypothetical glycosyl transferase YGL074Wgi\|2131571\|pir\|\|S64051 hypothetical prot [nrdb_11152004_Chlamydomonas] ALG13 SGDID:S000003015, Chr VII from 411555-412163, Verified ORF, "Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar t [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] UDP-N-acetylglucosamine transferase subunit ALG13 (Asparagine-linked glycosylation protein 13) [nr-271106-contam.fasta] Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; Alg13p [Saccharomyces c [nr-271106-contam.fasta] ALG13 SGDID:S000003015, Chr VII from 411555-412163, Verified ORF, "Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases" [yeast-bovrabprots.fasta] [chemostatdb.fasta] UDP-N-acetylglucosamine transferase subunit ALG13 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALG13 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ALG13 SGDID:S000003015, Chr VII from 411552-412160, Genome Release 64-1-1, Verified ORF, "Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases" [yeast-030211-contam.fasta] ALG13 SGDID:S000003015, Chr VII from 411552-412160, Genome Release 64-2-1, Verified ORF, "Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress" [20141208_orf_trans.fasta] YGL047W SGDID:S000003015, Chr VII from 411552-412160, Genome Release 64-1-1, Verified ORF, "Catalytic component of UDP-GlcNAc transferase, required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	202 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA] nucleus [IDA] extrinsic to endoplasmic reticulum membrane [IDA] cytoplasm [IDA ] UDP-N-acetylglucosamine transferase complex [IPI ] cytosol [IDA] endoplasmic reticulum [IDA]
Biological Process:	dolichol-linked oligosaccharide biosynthetic process [IDA][IMP ][ISS ]
Molecular Function:	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity [IDA][IMP ][ISS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGIIEEKALF VTCGATVPFP KLVSCVLSDE FCQELIQYGF VRLIIQFGRN YSSEFEHLVQ  60
   61 ERGGQRESQK IPIDQFGCGD TARQYVLMNG KLKVIGFDFS TKMQSIIRDY SDLVISHAGT 120
  121 GSILDSLRLN KPLIVCVNDS LMDNHQQQIA DKFVELGYVW SCAPTETGLI AGLRASQTEK 180
  181 LKPFPVSHNP SFERLLVETI YS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		ALG13 ALG14 ALG2 ALG6 DIE2 OST5 SHR5 STT3 WBP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ALG13	No Comments	Hazbun TR, et al. (2003)
View Run	ALG13	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ALG13	SWI5	1	Hazbun TR, et al. (2003)
View Screen	ALG13	YER187W	1	Hazbun TR, et al. (2003)
View Screen	ALG13	RME1	1	Hazbun TR, et al. (2003)
View Screen	ALG13	SNF6	1	Hazbun TR, et al. (2003)
View Screen	ALG13	YLR227W-A, YML04...	1	Hazbun TR, et al. (2003)
View Screen	ALG13	PPR1	1	Hazbun TR, et al. (2003)
View Screen	ALG13	NSL1	1	Hazbun TR, et al. (2003)
View Screen	ALG13	YBL081W	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ALG13 and SPC42	Hazbun TR, et al. (2003)
View Data	ALG13	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..202]	PSI-BLAST	27.522879	Peptidoglycan biosynthesys glycosyltransferase MurG

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.80

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2