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Protein Overview: RPO41

General Information:
Name(s) found: RPO41 / YFL036W [SGD]
Description(s) found:
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  • gi|836718|dbj|BAA09203.1| mithochondrial DNA-directed RNA polymerase [Saccharomyces cerevisiae] [NCBI NR]
  • gi|1589360|prf||2210408E RNA polymerase [NCBI NR]
  • gi|14318482|ref|NP_116617.1| Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Rpo41p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S56218 DNA-directed RNA polymerase (EC 2.7.7.6), mitochondrial - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • RPO41 DNA-directed RNA polymerase, mitochondrial [mips]
  • Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition [SGD]
  • RPO41, Chr VI from 58781-62836 [yeast_orfs2]
  • Chr VI from 58781-62836 [yeastorf3.fasta]
  • RPO41 SGDID:S0001858, Chr VI from 58781-62836, Verified ORF [yeastflysequest.fasta]
  • Rpo41p [Saccharomyces cerevisiae]gi|1084540|pir||S56218 DNA-directed RNA polymerase (EC 2.7.7.6), mitochondrial - yeast (Saccharomyces cerevisiae)gi|836718|dbj|BAA09203.1| mithochondrial DNA-directed RNA polymerase [Saccharomyces cerevisiae]gi|1589360| [nrdb_11152004_Chlamydomonas]
  • RPO41 SGDID:S000001858, Chr VI from 58781-62836, Verified ORF, "Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Rpo41p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • RNA polymerase [nr-271106-contam.fasta]
  • mithochondrial DNA-directed RNA polymerase [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • DNA-directed RNA polymerase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPO41 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • RPO41 SGDID:S000001858, Chr VI from 58782-62837, Genome Release 64-1-1, Verified ORF, "Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition" [yeast-030211-contam.fasta]
  • RPO41 SGDID:S000001858, Chr VI from 58782-62837, Genome Release 64-2-1, Verified ORF, "Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation" [20141208_orf_trans.fasta]
  • YFL036W SGDID:S000001858, Chr VI from 58782-62837, Genome Release 64-1-1, Verified ORF, "Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
  • DNA-directed RNA polymerase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPO41 PE=3 SV=2 [Swanson-Carlisle-Scereplusabalone-contam.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 1351 amino acids

    Gene Ontology:
    Cellular Component: mitochondrial matrix [IDALink to PubMed articleLink to PubMed article]
    mitochondrion [IDALink to PubMed article]
    mitochondrial nucleoid [IDALink to PubMed article]
    Biological Process: transcription from mitochondrial promoter [IMPLink to PubMed article]
    mitochondrial genome maintenance [IMPLink to PubMed article]
    Molecular Function: DNA-directed RNA polymerase activity [IDALink to PubMed articleLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRPAYKSLV KTSLLQRRLI SSKGSKLFKP SPDSTSTILI SEDPLVTGSS PTSSTTSGII  60
   61 SSNDFPLFNK NRKDAKSSIS YQWKNPSELE FDPFNKSHAS AVTSMTRTRD VMQLWSLLEA 120
  121 CLQSNLMKRA FSILESLYLV PEHKQRFIED YNMYLNSFSK NDPNFPILKM NEKLTNDLET 180
  181 SFKDVNYNDK TLAIMIHHAL NFHSTTSSML LKPIISAYLK MSVNGIREIF SCLDILTISD 240
  241 LNILMNDLKV ITPSQLPNSV RPILESLTLS PTPVNNIENE EGLNKVEAEN DSKLHKASNA 300
  301 SSDSIKKPSL DPLREVSFHG STEVLSKDAE KLIAVDTIGM RVIRHTLLGL SLTPEQKEQI 360
  361 SKFKFDANDN VLKMKPTKND DNNNSINFFE IYNSLPTLEE KKAFESALNI FNQDRQKVLE 420
  421 NRATEAARER WKHDFEEAKA RGDISIEKNL NVKLWKWYNE MLPLVKEEIN HCRSLLSEKL 480
  481 SDKKGLNKVD TNRLGYGPYL TLIDPGKMCV ITILELLKLN STGGVIEGMR TARAVISVGK 540
  541 AIEMEFRSEQ VLKSESQAFR DVNKKSPEFK KLVQNAKSVF RSSQIEQSKI LWPQSIRARI 600
  601 GSVLISMLIQ VAKVSVQGVD PVTKAKVHGE APAFAHGYQY HNGSKLGVLK IHKTLIRQLN 660
  661 GERLIASVQP QLLPMLVEPK PWVNWRSGGY HYTQSTLLRT KDSPEQVAYL KAASDNGDID 720
  721 RVYDGLNVLG RTPWTVNRKV FDVVSQVWNK GEGFLDIPGA QDEMVLPPAP PKNSDPSILR 780
  781 AWKLQVKTIA NKFSSDRSNR CDTNYKLEIA RAFLGEKLYF PHNLDFRGRA YPLSPHFNHL 840
  841 GNDMSRGLLI FWHGKKLGPS GLKWLKIHLS NLFGFDKLPL KDRVAFTESH LQDIKDSAEN 900
  901 PLTGDRWWTT ADKPWQALAT CFELNEVMKM DNPEEFISHQ PVHQDGTCNG LQHYAALGGD 960
  961 VEGATQVNLV PSDKPQDVYA HVARLVQKRL EIAAEKGDEN AKILKDKITR KVVKQTVMTN1020
 1021 VYGVTYVGAT FQIAKQLSPI FDDRKESLDF SKYLTKHVFS AIRELFHSAH LIQDWLGESA1080
 1081 KRISKSIRLD VDEKSFKNGN KPDFMSSVIW TTPLGLPIVQ PYREESKKQV ETNLQTVFIS1140
 1141 DPFAVNPVNA RRQKAGLPPN FIHSLDASHM LLSAAECGKQ GLDFASVHDS YWTHASDIDT1200
 1201 MNVVLREQFI KLHEVDLVLR LKEEFDQRYK NYVKIGKLKR STDLAQKIIR IRKDLSRKLG1260
 1261 RSTTLADEIY FEKKRQELLN SPLIEDRNVG EKMVTTVSLF EDITDLDALE LENGGDENSG1320
 1321 MSVLLPLRLP EIPPKGDFDV TVLRNSQYFF S

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Qiu et al. (2008) ACO1 ILV5 MTF1 RIM1 RPO41 YHM2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RPO41 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..379] deduced N/A No confident structure predictions are available.
    2 View Details [380..423]
    [547..591]
    [671..696]
    [805..820]    		 PSI-BLAST 1703.0103 T7 RNA polymerase
    3 View Details [424..546]
    [592..670]
    [697..733]
    [769..804]
    [821..840]
    [1124..1144]    		 PSI-BLAST 1703.0103 T7 RNA polymerase
    4 View Details [734..768]
    [841..945]
    [1167..1205]    		 PSI-BLAST 1703.0103 T7 RNA polymerase
    5 View Details [946..1123]
    [1145..1166]
    [1206..1259]    		 PSI-BLAST 1703.0103 T7 RNA polymerase
    6 View Details [1260..1351] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • nucleotidyltransferase activity
    		•  7.61500320688433 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  7.08533444189463 bayes_pls_golite062009
  • RNA polymerase activity
    		•  7.08533444189463 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  5.40114148359009 bayes_pls_golite062009
  • transferase activity
    		•  4.7475061982508 bayes_pls_golite062009
  • DNA-directed DNA polymerase activity
    		•  3.58831088586896 bayes_pls_golite062009
  • DNA polymerase activity
    		•  3.3731931763308 bayes_pls_golite062009
  • binding
    		•  1.92492422240469 bayes_pls_golite062009
  • DNA binding
    		•  1.87721092677049 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.64235873416087 bayes_pls_golite062009
  • chromatin binding
    		•  1.32386525961438 bayes_pls_golite062009
  • catalytic activity
    		•  1.04899053677564 bayes_pls_golite062009
  • protein binding
    		•  0.561579019896047 bayes_pls_golite062009
  • exonuclease activity
    		•  0.16454326755539 bayes_pls_golite062009
  • 3'-5' exonuclease activity
    		•  0.0700154911930899 bayes_pls_golite062009
  • nuclease activity
    		•  0.0574785418962505 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle