Protein Overview: TRM8

General Information:
Name(s) found:	TRM8 / YDL201W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320000\|ref\|NP_010080.1\| Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA; Trm8p [Saccharomyces cerevisiae] [NCBI NR] gi\|32171782\|sp\|Q12009.1\|TRMB_YEAST RecName: Full=tRNA (guanine-N(7)-)-methyltransferase; AltName: Full=tRNA(m7G46)-methyltransferase; AltName: Full=Transfer RNA methyltransferase 8 [NCBI NR] pir\|\|S67760 hypothetical protein YDL201w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1431332\|emb\|CAA98779.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1429356\|emb\|CAA67468.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] tRNA (guanine-N(7)-)-methyltransferase OS=Saccharomyces cerevisiae GN=TRM8 PE=1 SV=1 [Swiss-Prot] TRM8 Part of complex required for 7-methylguanosine modification [mips] Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA [SGD] YDL201W, Chr IV from 99562-100422 [yeast_orfs2] Chr IV from 99562-100422 [yeastorf3.fasta] (Q12009) tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33) (tRNA(m7G46)-methyltransferase) [4932.FASTACCERV.txt] TRM8 SGDID:S0002360, Chr IV from 99562-100422, Verified ORF [yeastflysequest.fasta] Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA [Saccharomyces cerevisiae]gi\|1429356\|emb\|CAA67468.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|1431332\|emb\|CAA98779 [nrdb_11152004_Chlamydomonas] TRM8 SGDID:S000002360, Chr IV from 99562-100422, Verified ORF, "Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA; Trm8p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] tRNA (guanine-N(7)-)-methyltransferase (tRNA(m7G46)-methyltransferase) [nr-271106-contam.fasta] [chemostatdb.fasta] tRNA (guanine-N(7)-)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRM8 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TRM8 SGDID:S000002360, Chr IV from 99561-100421, Genome Release 64-1-1, Verified ORF, "Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA" [yeast-030211-contam.fasta] TRM8 SGDID:S000002360, Chr IV from 99561-100421, Genome Release 64-2-1, Verified ORF, "Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p" [20141208_orf_trans.fasta] YDL201W SGDID:S000002360, Chr IV from 99561-100421, Genome Release 64-1-1, Verified ORF, "Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	286 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	ribosome biogenesis [RCA] tRNA methylation [IMP]
Molecular Function:	protein binding [IPI] tRNA (guanine-N7-)-methyltransferase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKAKPLSQDP GSKRYAYRIN KEENRKELKH VKINESSLVQ EGQKIDLPKK RYYRQRAHSN  60
   61 PFSDHQLEYP VSPQDMDWSK LYPYYKNAEN GQMTKKVTIA DIGCGFGGLM IDLSPAFPED 120
  121 LILGMEIRVQ VTNYVEDRII ALRNNTASKH GFQNINVLRG NAMKFLPNFF EKGQLSKMFF 180
  181 CFPDPHFKQR KHKARIITNT LLSEYAYVLK EGGVVYTITD VKDLHEWMVK HLEEHPLFER 240
  241 LSKEWEENDE CVKIMRNATE EGKKVERKKG DKFVACFTRL PTPAIL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		TRM8 TRM82
View Details	Krogan NJ, et al. (2006)		BNA5 HXK1 TRM8 TRM82
View Details	Qiu et al. (2008)		GCD10 GCD14 NCL1 TRM112 TRM8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	AML1	TRM8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TRM8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..72]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[73..286]	PSI-BLAST	18.39794	No description for 1j4fA_ was found.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
methyltransferase activity	4.15445027778357	bayes_pls_golite062009
N-methyltransferase activity	4.07806017452628	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	4.07153733729662	bayes_pls_golite062009
protein methyltransferase activity	3.92746815303714	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	3.39429041855776	bayes_pls_golite062009
RNA methyltransferase activity	3.07428840534971	bayes_pls_golite062009
histone methyltransferase activity	2.99461585647365	bayes_pls_golite062009
tRNA methyltransferase activity	2.5931020126592	bayes_pls_golite062009
DNA-methyltransferase activity	2.43145108778901	bayes_pls_golite062009
arginine N-methyltransferase activity	2.41876112831625	bayes_pls_golite062009
protein-arginine N-methyltransferase activity	2.41876112831625	bayes_pls_golite062009
rRNA methyltransferase activity	2.29640050660273	bayes_pls_golite062009
transferase activity	2.22303238506686	bayes_pls_golite062009
histone-arginine N-methyltransferase activity	2.04296107639729	bayes_pls_golite062009
tRNA (cytosine-5-)-methyltransferase activity	1.88889414729709	bayes_pls_golite062009
tRNA (cytosine)-methyltransferase activity	1.88889414729709	bayes_pls_golite062009
protein-arginine omega-N monomethyltransferase activity	1.66871163498737	bayes_pls_golite062009
tRNA (adenine-N1-)-methyltransferase activity	1.53559006858264	bayes_pls_golite062009
tRNA (adenine)-methyltransferase activity	1.53559006858264	bayes_pls_golite062009
catalytic activity	1.25333584501255	bayes_pls_golite062009
histone methyltransferase activity (H4-R3 specific)	1.1103522853299	bayes_pls_golite062009
transcription regulator activity	0.914549843668268	bayes_pls_golite062009
protein-arginine omega-N asymmetric methyltransferase activity	0.89325812193225	bayes_pls_golite062009
DNA binding	0.86027591375666	bayes_pls_golite062009
rRNA (guanine) methyltransferase activity	0.720972789045101	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	0.703178429468566	bayes_pls_golite062009
nucleic acid binding	0.616949327277483	bayes_pls_golite062009
rRNA (adenine) methyltransferase activity	0.533090627724779	bayes_pls_golite062009
rRNA (adenine-N6,N6-)-dimethyltransferase activity	0.455449410561251	bayes_pls_golite062009
RNA binding	0.417524996559564	bayes_pls_golite062009
binding	0.383237930789959	bayes_pls_golite062009
tRNA (guanine) methyltransferase activity	0.203168546434167	bayes_pls_golite062009
rRNA (uridine) methyltransferase activity	0.0858581024725429	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2