Protein Overview: MGT1

General Information:
Name(s) found:	MGT1 / YDL200C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320001\|ref\|NP_010081.1\| DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage; Mgt1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|XUBYMC methylated-DNA-protein-cysteine S-methyltransferase (EC 2.1.1.63) [similarity] - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|171949\|gb\|AAA34780.1\| O-6-methylguanine-DNA methyltransferase [Saccharomyces cerevisiae] [NCBI NR] gi\|1431330\|emb\|CAA98778.1\| MGT1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1429357\|emb\|CAA67469.1\| methylated-DNA-protein-cysteine S-methyl-transferase [Saccharomyces cerevisiae] [NCBI NR] gi\|127072\|sp\|P26188\|MGMT_YEAST Methylated-DNA--protein-cysteine methyltransferase (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase) [NCBI NR] Methylated-DNA--protein-cysteine methyltransferase - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] MGT1 O6-methylguanine DNA repair methyltransferase [mips] DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage [SGD] MGT1, Chr IV from 100502-101122, reverse complement [yeast_orfs2] Chr IV from 100502-101122, reverse complement [yeastorf3.fasta] Chr IV from 101122-100502, reverse complement [yeastorf5.fasta] (P26188) Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) [4932.FASTACCERV.txt] MGT1 SGDID:S0002359, Chr IV from 101122-100502, reverse complement, Verified ORF [yeastflysequest.fasta] 6-O-methylguanine-DNA methylase [Saccharomyces cerevisiae]gi\|1429357\|emb\|CAA67469.1\| methylated-DNA-protein-cysteine S-methyl-transferase [Saccharomyces cerevisiae]gi\|1431330\|emb\|CAA98778.1\| MGT1 [Saccharomyces cerevisiae]gi\|2144396\|pir\|\|XUBYMC methyla [nrdb_11152004_Chlamydomonas] (P26188) Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase) [cerv4932.FASTAC] MGT1 SGDID:S000002359, Chr IV from 101122-100502, reverse complement, Verified ORF, "DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MGT1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] O-6-methylguanine-DNA methyltransferase [nr-271106-contam.fasta] methylated-DNA-protein-cysteine S-methyl-transferase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Methylated-DNA--protein-cysteine methyltransferase (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase) [nr-271106-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	206 amino acids
Gene Ontology:
Cellular Component:	nucleus [IC]
Biological Process:	DNA dealkylation involved in DNA repair [IMP]
Molecular Function:	methylated-DNA-[protein]-cysteine S-methyltransferase activity [IDA ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MHKKKIENGR IFDLNGPTMK ELLYYTFIET EVTGAFLVFR EKTQNLVFAS LGNDKLFLLG  60
   61 KVEGFLKKHE KQDTMYDLQE LKEAETYKKS IENYTICLEN KMPLPSGAIP FEFLFGTDFQ 120
  121 RKVWNELLNV EHGHVVTYGD IAKRIGKPTA ARSVGRACGS NNLALLVPCH RIVGSNRKLT 180
  181 GYKWSCKLKE QLLNNEKENS LSLSRL

Protein Complex Data

No complex found for this protein.

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	DMC1	MGT1	1	Unpublished Fields Lab Data
View Screen	PRR1	MGT1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..114]	PSI-BLAST	232.69897	Ada DNA repair protein
2	View Details	[115..206]	PSI-BLAST	232.69897	Ada DNA repair protein

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
methylated-DNA-[protein]-cysteine S-methyltransferase activity	3.81641972619245	bayes_pls_golite062009
S-methyltransferase activity	2.66007025371147	bayes_pls_golite062009
catalytic activity	1.90838528948382	bayes_pls_golite062009
DNA binding	1.54553435062678	bayes_pls_golite062009
nucleic acid binding	1.49720271661049	bayes_pls_golite062009
binding	1.30758209326705	bayes_pls_golite062009
transcription regulator activity	1.26332746912934	bayes_pls_golite062009
transferase activity	1.22815682532854	bayes_pls_golite062009
methyltransferase activity	1.14625481859451	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	1.13184530961695	bayes_pls_golite062009
transcription factor activity	0.99095733801135	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2