Protein Overview: ILV6

General Information:
Name(s) found:	ILV6 / YCL009C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319837\|ref\|NP_009918.1\| Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria; Ilv6p [Saccharomyces cerevisiae [NCBI NR] gi\|3041696\|sp\|P25605.2\|ILV6_YEAST RecName: Full=Acetolactate synthase small subunit, mitochondrial; AltName: Full=Acetohydroxy-acid synthase small subunit; Short=AHAS; Short=ALS; Flags: Precursor [NCBI NR] gi\|1907135\|emb\|CAA42350.1\| acetolactate synthase, regulatory subunit [Saccharomyces cerevisiae] [NCBI NR] Acetolactate synthase small subunit, mitochondrial OS=Saccharomyces cerevisiae GN=ILV6 PE=1 SV=2 [Swiss-Prot] ILV6 Acetolactate synthase, regulatory subunit [mips] Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria [SGD] ILV6, Chr III from 104614-105543, reverse complement [yeast_orfs2] Chr III from 104614-105543, reverse complement [yeastorf3.fasta] Chr III from 105543-104614, reverse complement [yeastorf5.fasta] (P25605) Acetolactate synthase small subunit, mitochondrial precursor (EC 2.2.1.6) (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS) [4932.FASTACCERV.txt] ILV6 SGDID:S0000515, Chr III from 105547-104618, reverse complement, Verified ORF [yeastflysequest.fasta] Ilv6p [Saccharomyces cerevisiae]gi\|1907135\|emb\|CAA42350.1\| acetolactate synthase, regulatory subunit [Saccharomyces cerevisiae]gi\|3041696\|sp\|P25605\|ILV6_YEAST Acetolactate synthase small subunit, mitochondrial precursor (AHAS) (Acetohydroxy-acid synthas [nrdb_11152004_Chlamydomonas] ILV6 SGDID:S000000515, Chr III from 105547-104618, reverse complement, Verified ORF, "Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ILV6 SGDID:S000000515, Chr III from 105548-104619, reverse complement, Verified ORF, "Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, [yeast-contam.fasta] acetolactate synthase, regulatory subunit [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria; Ilv6p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Acetolactate synthase small subunit, mitochondrial precursor (AHAS) (Acetohydroxy-acid synthase small subunit) (ALS) [nr-271106-contam.fasta] ILV6 SGDID:S000000515, Chr III from 105548-104619, reverse complement, Verified ORF, "Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Acetolactate synthase small subunit, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ILV6 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ILV6 SGDID:S000000515, Chr III from 105548-104619, Genome Release 64-1-1, reverse complement, Verified ORF, "Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria" [yeast-030211-contam.fasta] ILV6 SGDID:S000000515, Chr III from 105548-104619, Genome Release 64-2-1, reverse complement, Verified ORF, "Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria" [20141208_orf_trans.fasta] YCL009C SGDID:S000000515, Chr III from 105548-104619, Genome Release 64-1-1, reverse complement, Verified ORF, "Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	309 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] acetolactate synthase complex [IDA] mitochondrial nucleoid [IDA]
Biological Process:	branched chain family amino acid biosynthetic process [IDA][ISS]
Molecular Function:	acetolactate synthase activity [IDA] enzyme regulator activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRSLLQSGH RRVVASSCAT MVRCSSSSTS ALAYKQMHRH ATRPPLPTLD TPSWNANSAV  60
   61 SSIIYETPAP SRQPRKQHVL NCLVQNEPGV LSRVSGTLAA RGFNIDSLVV CNTEVKDLSR 120
  121 MTIVLQGQDG VVEQARRQIE DLVPVYAVLD YTNSEIIKRE LVMARISLLG TEYFEDLLLH 180
  181 HHTSTNAGAA DSQELVAEIR EKQFHPANLP ASEVLRLKHE HLNDITNLTN NFGGRVVDIS 240
  241 ETSCIVELSA KPTRISAFLK LVEPFGVLEC ARSGMMALPR TPLKTSTEEA ADEDEKISEI 300
  301 VDISQLPPG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		ILV2 ILV6
View Details	Qiu et al. (2008)		ILV2 ILV6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC23	ILV6	1	Unpublished Fields Lab Data
View Screen	SPT21	ILV6	1	Unpublished Fields Lab Data
View Screen	DOT1	ILV6	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ILV6	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..190]	ORFEUS	10.87	No description for 1in9A was found.
2	View Details	[191..309]	MSA	1.056986	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
acetolactate synthase activity	14.1096734416761	bayes_pls_golite062009
transferase activity, transferring aldehyde or ketonic groups	12.4176994384119	bayes_pls_golite062009
transcription regulator activity	1.94365770896971	bayes_pls_golite062009
binding	1.29694755595984	bayes_pls_golite062009
nucleic acid binding	1.1911993852748	bayes_pls_golite062009
DNA binding	1.11572529990201	bayes_pls_golite062009
catalytic activity	0.645256181736122	bayes_pls_golite062009
transcription factor activity	0.595085179690602	bayes_pls_golite062009
protein binding	0.319036476490264	bayes_pls_golite062009
transcription repressor activity	0.11276971754733	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2