Protein Overview: CHK1

General Information:
Name(s) found:	CHK1 / YBR274W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319751\|ref\|NP_009833.1\| Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase [NCBI NR] pir\|\|S46155 probable serine/threonine-specific protein kinase (EC 2.7.1.-) YBR274w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|585344\|sp\|P38147.1\|CHK1_YEAST RecName: Full=Serine/threonine-protein kinase CHK1; AltName: Full=Checkpoint kinase 1 [NCBI NR] gi\|536713\|emb\|CAA85237.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|4325043\|gb\|AAD17231.1\| checkpoint protein kinase homolog Chk1 [Saccharomyces cerevisiae] [NCBI NR] Serine/threonine-protein kinase CHK1 OS=Saccharomyces cerevisiae GN=CHK1 PE=1 SV=1 [Swiss-Prot] CHK1 Regulats inhibitory Cdk phosphorylation of Pds1 [mips] Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase [SGD] CHK1, Chr II from 749550-751133 [yeast_orfs2] Chr II from 749550-751133 [yeastorf3.fasta] Chr II from 749549-751132 [yeastorf4.fasta] Chr II from 749547-751130 [yeastorf5.fasta] (P38147) Serine/threonine-protein kinase CHK1 (EC 2.7.1.-) (Checkpoint kinase 1) [4932.FASTACCERV.txt] CHK1 SGDID:S0000478, Chr II from 749551-751134, Verified ORF [yeastflysequest.fasta] CHK1 SGDID:S0000478, Chr II from 749589-751172, Verified ORF [10112005cervSGD.fasta] Chk1p [Saccharomyces cerevisiae]gi\|536713\|emb\|CAA85237.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|585344\|sp\|P38147\|CHK1_YEAST Serine/threonine-protein kinase CHK1 (Checkpoint kinase 1)gi\|4325043\|gb\|AAD17231.1\| checkpoint protein kinase hom [nrdb_11152004_Chlamydomonas] (P38147) Serine/threonine-protein kinase CHK1 (EC 2.7.1.37) (Checkpoint kinase 1) [cerv4932.FASTAC] CHK1 SGDID:S000000478, Chr II from 749589-751172, Verified ORF, "DNA damage checkpoint effector kinase, mediates mitotic cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer kinase Mec1p; homolog of S. pombe and m [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CHK1 SGDID:S000000478, Chr II from 749589-751172, Verified ORF, "Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Serine/threonine-protein kinase CHK1 (Checkpoint kinase 1) [nr-271106-contam.fasta] checkpoint protein kinase homolog Chk1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA damage checkpoint effector kinase, mediates mitotic cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer kinase Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase; Chk1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CHK1 SGDID:S000000478, Chr II from 749589-751172, Verified ORF, "Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase" [yeast-bovrabprots.fasta] CHK1 SGDID:S000000478, Chr II from 749589-751172, Verified ORF, "DNA damage checkpoint effector kinase, mediates mitotic cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer kinase Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase" [yeast-contam_custom.fasta] [chemostatdb.fasta] Serine/threonine-protein kinase CHK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CHK1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CHK1 SGDID:S000000478, Chr II from 749594-751177, Genome Release 64-1-1, Verified ORF, "Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase" [yeast-030211-contam.fasta] CHK1 SGDID:S000000478, Chr II from 749594-751177, Genome Release 64-2-1, Verified ORF, "Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase" [20141208_orf_trans.fasta] TR8444\|c0_g1_i1\|g.5838 ORF TR8444\|c0_g1_i1\|g.5838 TR8444\|c0_g1_i1\|m.5838 type:complete len:528 (+) TR8444\|c0_g1_i1:61-1644(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6286\|c0_g1_i1\|g.4470 ORF TR6286\|c0_g1_i1\|g.4470 TR6286\|c0_g1_i1\|m.4470 type:complete len:528 (+) TR6286\|c0_g1_i1:61-1644(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YBR274W SGDID:S000000478, Chr II from 749594-751177, Genome Release 64-1-1, Verified ORF, "Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4827\|g.3650 ORF asmbl_4827\|g.3650 asmbl_4827\|m.3650 type:complete len:528 (+) asmbl_4827:59-1642(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5159\|g.3203 ORF ID=asmbl_5159\|g.3203 ID=asmbl_5159\|m.3203 type:complete len:528 (+) ID=asmbl_5159:59-1642(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_4833\|g.3142 ORF ID=asmbl_4833\|g.3142 ID=asmbl_4833\|m.3142 type:complete len:528 (+) ID=asmbl_4833:59-1642(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	527 amino acids
Gene Ontology:
Cellular Component:	nucleus [IPI]
Biological Process:	DNA damage checkpoint [IMP] protein amino acid phosphorylation [IMP][ISS]
Molecular Function:	protein kinase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP TCKKMGLSDK  60
   61 DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG GDLFDKIEPD VGVDSDVAQF 120
  121 YFQQLVSAIN YLHVECGVAH RDIKPENILL DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ 180
  181 RGSPPYMAPE VLYSEEGYYA DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG 240
  241 NLNWGPWSKI EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL 300
  301 AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF QTVSNTQRAF 360
  361 TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND CNELVKRHLQ FNPNKLTKFY 420
  421 TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN FERLCELLGY DNVFPLIINI KTKSNGGYQL 480
  481 CGSISIIKIE EELKSVGFER KTGDPLEWRR LFKKISTICR DIILIPN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BCY1 CHK1 HSL1 TPK1 TPK2
View Details	Ho Y, et al. (2002)		CHK1 CTR1 GFA1 YLR152C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD9	CHK1	2	Unpublished Fields Lab Data
View Screen	CHK1	RAD9	2	Unpublished Fields Lab Data
View Screen	CHK1	RAD3	2	Unpublished Fields Lab Data
View Screen	CHK1	SDS3	2	Unpublished Fields Lab Data
View Screen	CHK1	SNX4	2	Unpublished Fields Lab Data
View Screen	CHK1	YNL234W	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CHK1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..64] [82..96]	PSI-BLAST	500.616428	Cell cycle checkpoint kinase chk1
2	View Details	[65..81] [97..106] [122..175] [287..307]	PSI-BLAST	500.616428	Cell cycle checkpoint kinase chk1
3	View Details	[107..121] [176..286]	PSI-BLAST	500.616428	Cell cycle checkpoint kinase chk1
4	View Details	[308..395]	PSI-BLAST	16.045757	Calmodulin
5	View Details	[396..527]	PSI-BLAST	16.045757	Calmodulin

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.93

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2