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View Structure Prediction Details

Protein: RAD1
Organism: Saccharomyces cerevisiae
Length: 1100 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for RAD1.

Description E-value Query
Range
Subject
Range
RAD1 - Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excisi...
RAD1_YEAST - DNA repair protein RAD1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD1 PE=1 SV=1
0.0 [1..1100] [1..1100]
gi|7657064, gi|6... - gi|7657064|ref|NP_056584.1| excision repair cross-complementing rodent repair deficiency, complement...
0.0 [84..1076] [7..914]
XPF_CRIGR - DNA repair endonuclease XPF OS=Cricetulus griseus GN=ERCC4 PE=2 SV=3
0.0 [86..1066] [9..904]
ERCC4 - excision repair cross-complementing rodent repair deficiency, complementation group 4
gi|182887847 - gi|182887847|gb|AAI60102.1| Excision repair cross-complementing rodent repair deficiency, complement...
0.0 [83..1066] [6..907]
gi|32766570, gi|... - gi|41054253|ref|NP_956079.1| excision repair cross-complementing rodent repair deficiency, complemen...
0.0 [91..1061] [4..874]

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Predicted Domain #1
Region A:
Residues: [1-299]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQLFYQGDS DDELQEELTR QTTQASQSSK IKNEDEPDDS NHLNEVENED SKVLDDDAVL  60
   61 YPLIPNEPDD IETSKPNIND IRPVDIQLTL PLPFQQKVVE NSLITEDALI IMGKGLGLLD 120
  121 IVANLLHVLA TPTSINGQLK RALVLVLNAK PIDNVRIKEA LEELSWFSNT GKDDDDTAVE 180
  181 SDDELFERPF NVVTADSLSI EKRRKLYISG GILSITSRIL IVDLLSGIVH PNRVTGMLVL 240
  241 NADSLRHNSN ESFILEIYRS KNTWGFIKAF SEAPETFVME FSPLRTKMKE LRLKNVLLW

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 12.522879
Match: 1qdeA_
Description: Initiation factor 4a
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [300-585]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PRFRVEVSSC LNATNKTSHN KVIEVKVSLT NSMSQIQFGL MECLKKCIAE LSRKNPELAL  60
   61 DWWNMENVLD INFIRSIDSV MVPNWHRISY ESKQLVKDIR FLRHLLKMLV TSDAVDFFGE 120
  121 IQLSLDANKP SVSRKYSESP WLLVDEAQLV ISYAKKRIFY KNEYTLEENP KWEQLIHILH 180
  181 DISHERMTNH LQGPTLVACS DNLTCLELAK VLNASNKKRG VRQVLLNKLK WYRKQREETK 240
  241 KLVKEVQSQD TFPENATLNV SSTFSKEQVT TKRRRTRGAS QVAAVE

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 3.0
Match: 1gm5A_
Description: RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [586-742]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 KLRNAGTNVD MEVVFEDHKL SEEIKKGSGD DLDDGQEENA ANDSKIFEIQ EQENEILIDD  60
   61 GDAEFDNGEL EYVGDLPQHI TTHFNKDLWA EHCNEYEYVD RQDEILISTF KSLNDNCSLQ 120
  121 EMMPSYIIMF EPDISFIRQI EVYKAIVKDL QPKVYFM

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 3.0
Match: 1gm5A_
Description: RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
Matching Structure (courtesy of the PDB):

Predicted Domain #4
Region A:
Residues: [743-819]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 YYGESIEEQS HLTAIKREKD AFTKLIRENA NLSHHFETNE DLSHYKNLAE RKLKLSKLRK  60
   61 SNTRNAGGQQ GFHNLTQ

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 28.0
Match: 1d9xA_
Description: Nucleotide excision repair enzyme UvrB
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
single-stranded DNA specific endodeoxyribonuclease activity 12.5797089932289 bayes_pls_golite062009
endodeoxyribonuclease activity 7.65460057398582 bayes_pls_golite062009
deoxyribonuclease activity 6.8949486660401 bayes_pls_golite062009
endonuclease activity 6.81743235298471 bayes_pls_golite062009
four-way junction helicase activity 4.48421726286311 bayes_pls_golite062009
DNA helicase activity 4.45575761476577 bayes_pls_golite062009
DNA strand annealing activity 3.51692202399009 bayes_pls_golite062009
excinuclease ABC activity 3.48615352002265 bayes_pls_golite062009
single-stranded DNA binding 3.44605832131927 bayes_pls_golite062009
structure-specific DNA binding 3.37084035853131 bayes_pls_golite062009
damaged DNA binding 2.98078816242626 bayes_pls_golite062009
DNA binding 2.97855002479178 bayes_pls_golite062009
helicase activity 2.83648239124034 bayes_pls_golite062009
nucleic acid binding 2.79236389919005 bayes_pls_golite062009
nuclease activity 2.20113675721212 bayes_pls_golite062009
binding 2.17324322139334 bayes_pls_golite062009
hydrolase activity 2.07039693890326 bayes_pls_golite062009
hydrolase activity, acting on ester bonds 1.69645042781713 bayes_pls_golite062009
double-stranded DNA binding 1.26255690081743 bayes_pls_golite062009
transposase activity 1.10140160488189 bayes_pls_golite062009
nucleoside-triphosphatase activity 1.05548921907953 bayes_pls_golite062009
pyrophosphatase activity 1.02983374615445 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides 1.01049916010827 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides 1.00920723797259 bayes_pls_golite062009
catalytic activity 0.816447099398022 bayes_pls_golite062009
protein binding 0.71091195096997 bayes_pls_golite062009

Predicted Domain #5
Region A:
Residues: [820-989]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DVVIVDTREF NASLPGLLYR YGIRVIPCML TVGDYVITPD ICLERKSISD LIGSLQNNRL  60
   61 ANQCKKMLKY YAYPTLLIEF DEGQSFSLEP FSERRNYKNK DISTVHPISS KLSQDEIQLK 120
  121 LAKLVLRFPT LKIIWSSSPL QTVNIILELK LGREQPDPSN AVILGTNKVR 

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 113.28043
Match: 1j22A_
Description: Putative ATP-dependent RNA helicase Hef, nuclease domain
Matching Structure (courtesy of the PDB):

Predicted Domain #6
Region A:
Residues: [990-1100]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SDFNSTAKGL KDGDNESKFK RLLNVPGVSK IDYFNLRKKI KSFNKLQKLS WNEINELIND  60
   61 EDLTDRIYYF LRTEKEEQEQ ESTDENLESP GKTTDDNALH DHHNDVPEAP V

[Run NCBI BLAST on this sequence.]

Detection Method: MSA

Shown below are all of our structure predictions for this domain.
Click here to view only most confident match.

    MCM
Score
GO
Score
GO
Term
SCOP
Match
SCOP Description
View Download 0.512 N/A N/A a.60.2 RuvA domain 2-like
View Download 0.411 N/A N/A c.55.1 Actin-like ATPase domain
View Download 0.381 N/A N/A f.14.1 Voltage-gated potassium channels
View Download 0.349 N/A N/A a.90.1 Transcription factor STAT-4 N-domain
View Download 0.326 N/A N/A a.60.9 lambda integrase-like, N-terminal domain
View Download 0.307 N/A N/A a.74.1 Cyclin-like
View Download 0.307 N/A N/A a.144.1 PABC (PABP) domain
View Download 0.264 N/A N/A a.60.7 5' to 3' exonuclease, C-terminal subdomain
View Download 0.262 N/A N/A a.61.1 Retroviral matrix proteins
View Download 0.261 N/A N/A a.1.1 Globin-like
View Download 0.261 N/A N/A a.74.1 Cyclin-like
View Download 0.256 N/A N/A a.27.1 Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases
View Download 0.253 N/A N/A a.48.3 Conserved domain common to transcription factors TFIIS, elongin A, CRSP70
View Download 0.252 N/A N/A a.7.3 Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain
View Download 0.233 N/A N/A a.80.1 DNA polymerase III clamp loader subunits, C-terminal domain
View Download 0.230 N/A N/A a.61.1 Retroviral matrix proteins
View Download 0.229 N/A N/A a.56.1 CO dehydrogenase ISP C-domain like
View Download 0.228 N/A N/A a.118.9 ENTH/VHS domain
View Download 0.219 N/A N/A a.68.1 Wiscott-Aldrich syndrome protein, WASP, C-terminal domain
View Download 0.215 N/A N/A a.74.1 Cyclin-like
View Download 0.209 N/A N/A a.24.17 Group V grass pollen allergen
View Download 0.205 N/A N/A a.4.5 "Winged helix" DNA-binding domain


YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle