Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
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PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 16 | 43 | 37.6% | 0.016 | 63004 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 12 | 33 | 56.9% | 0.0246 | 32498 |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 12 | 49 | 42.4% | 0.0327 | 36711 |
[ |
YPK9 | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... | 11 | 14 | 10.7% | 0.00201 | 166749 |
[ |
NYV1 | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... | 8 | 32 | 56.5% | 0.0267 | 28964 |
[ |
FRA1 | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... | 7 | 7 | 13.5% | 0.00197 | 84924 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 6 | 16 | 35.5% | 0.0156 | 24668 |
[ |
YPF1 | Putative protein of unknown function with similarity to a human minor histocompatibility antigen and... | 6 | 9 | 15.2% | 0.00324 | 67525 |
[ |
MNR2 | Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... | 6 | 8 | 10.2% | 0.00174 | 109736 |
[ |
VNX1 | Low affinity vacuolar membrane localized monovalent cation/H+ antiporter; member of the calcium exch... | 4 | 5 | 7.4% | 0.00116 | 102499 |
[ |
FPS1 | Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glyce... | 4 | 4 | 7.2% | 0.00126 | 73877 |
[ |
MAM3 | Protein required for normal mitochondrial morphology, has similarity to hemolysins | 3 | 7 | 11.3% | 0.00209 | 77712 |
[ |
ZRC1 | Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... | 3 | 5 | 9.7% | 0.00239 | 48345 |
[ |
PHO91 | Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... | 3 | 4 | 3.8% | 9.45E-4 | 99490 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 3 | 3 | 2.5% | 4.18E-4 | 171120 |
[ |
YNL217W | Hypothetical protein | 2 | 3 | 8.6% | 0.00194 | 37151 |
[ |
UGA1 | Gamma-aminobutyrate (GABA) transaminase (4-aminobutyrate aminotransferase) involved in the 4-aminobu... | 2 | 3 | 7.4% | 0.00134 | 52946 |
[ |
AVT3 | Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seve... | 2 | 3 | 6.8% | 9.15E-4 | 75459 |
[ |
ZRT3 | Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm whe... | 2 | 4 | 5.6% | 0.00168 | 55403 |
[ |
CPS1 | Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions | 2 | 3 | 4.5% | 0.0011 | 64597 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 2 | 2 | 4.4% | 8.45E-4 | 54545 |
[ |
YCK3 | Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... | 2 | 2 | 4.2% | 8.06E-4 | 60261 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 2 | 6 | 3.4% | 0.00216 | 64382 |
[ |
YDR089W | Protein of unknown function; deletion confers resistance to Nickel | 2 | 2 | 3.1% | 4.86E-4 | 98712 |