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Complex Overview

From Publication: Ho Y. et al. (2002) Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature. 2002 Jan 10;415(6868):180-3.
Notes: This molecular complex record represents a population of complexes co-purified by immunoprecipitation of FLAG-tagged Rad3. The topolgies of protein complexes in this experiment are unknown.
Complex Size: 14 proteins

Complex Member Proteins

Protein

GO Cellular Component GO Biological Process GO Molecular Function
AAC3
  • mitochondrion
  • mitochondrial inner membrane
  • anaerobic respiration
  • ATP:ADP antiporter activity
  • ACO1
  • mitochondrial matrix
  • mitochondrion
  • cytosol
  • mitochondrial nucleoid
  • glutamate biosynthetic process
  • response to drug
  • citrate metabolic process
  • propionate metabolic process
  • tricarboxylic acid cycle
  • mitochondrial genome maintenance
  • aconitate hydratase activity
  • ATP3
  • mitochondrial proton-transporting ATP synthase, central stalk
  • mitochondrion
  • ATP synthesis coupled proton transport
  • hydrogen ion transporting ATP synthase activity, rotational mechanism
  • CCL1
  • mitochondrion
  • holo TFIIH complex
  • negative regulation of transcription from RNA polymerase II promoter, mitotic
  • transcription initiation from RNA polymerase II promoter
  • cyclin-dependent protein kinase regulator activity
  • general RNA polymerase II transcription factor activity
  • HOR2
  • nucleus
  • cytoplasm
  • carbohydrate metabolic process
  • response to osmotic stress
  • glycerol biosynthetic process
  • glycerol-1-phosphatase activity
  • HXT6
  • mitochondrion
  • plasma membrane
  • hexose transport
  • fructose transmembrane transporter activity
  • glucose transmembrane transporter activity
  • mannose transmembrane transporter activity
  • LSC1
  • mitochondrion
  • mitochondrial nucleoid
  • tricarboxylic acid cycle
  • succinyl-CoA metabolic process
  • succinate-CoA ligase (ADP-forming) activity
  • MET18
  • nucleoplasm
  • telomere maintenance
  • nucleotide-excision repair
  • transcription from RNA polymerase II promoter
  • methionine metabolic process
  • RNA polymerase II transcription factor activity
  • RAD3
  • holo TFIIH complex
  • nucleotide-excision repair factor 3 complex
  • negative regulation of transcription from RNA polymerase II promoter, mitotic
  • nucleotide-excision repair, DNA duplex unwinding
  • nucleotide-excision repair
  • transcription initiation from RNA polymerase II promoter
  • DNA helicase activity
  • general RNA polymerase II transcription factor activity
  • RHR2
  • nucleus
  • cytoplasm
  • response to drug
  • response to osmotic stress
  • glycerol biosynthetic process
  • glycerol-1-phosphatase activity
  • RPN1
  • nucleus
  • proteasome regulatory particle, base subcomplex
  • cytoplasm
  • endoplasmic reticulum
  • ubiquitin-dependent protein catabolic process
  • endopeptidase activity
  • protein binding, bridging
  • RPT3
  • proteasome regulatory particle, base subcomplex
  • ubiquitin-dependent protein catabolic process
  • ATPase activity
  • endopeptidase activity
  • TFP1
  • vacuolar proton-transporting V-type ATPase, V1 domain
  • fungal-type vacuole membrane
  • protein metabolic process
  • vacuolar acidification
  • intron homing
  • proton-transporting ATPase activity, rotational mechanism
  • endodeoxyribonuclease activity
  • THI22
  • cellular_component
  • thiamin biosynthetic process
  • phosphomethylpyrimidine kinase activity
  • Cellular Component Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    proteasome regulatory particle, base subcomplex 1.6406E-4 14 9 2 6292
    holo TFIIH complex 2.0481E-4 14 10 2 6292
    proton-transporting two-sector ATPase complex, catalytic domain 3.5365E-4 14 13 2 6292
    proteasome regulatory particle 1.1326E-3 14 23 2 6292
    proteasome accessory complex 1.1326E-3 14 23 2 6292
    nucleoid 1.4494E-3 14 26 2 6292
    mitochondrial nucleoid 1.4494E-3 14 26 2 6292
    proton-transporting two-sector ATPase complex 2.6233E-3 14 35 2 6292
    proteasome complex 4.8918E-3 14 48 2 6292
    DNA-directed RNA polymerase II, holoenzyme 6.611E-3 14 56 2 6292
    mitochondrial proton-transporting ATP synthase, central stalk 6.6614E-3 14 3 1 6292
    proton-transporting ATP synthase, central stalk 6.6614E-3 14 3 1 6292

    Biological Process Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    generation of precursor metabolites and energy 2.0034E-6 14 195 6 6292
    energy derivation by oxidation of organic compounds 1.1735E-5 14 149 5 6292
    polyol biosynthetic process 2.7517E-5 14 4 2 6292
    alditol biosynthetic process 2.7517E-5 14 4 2 6292
    glycerol biosynthetic process 2.7517E-5 14 4 2 6292
    regulation of transcription from RNA polymerase II promoter, mitotic 1.2776E-4 14 8 2 6292
    negative regulation of transcription from RNA polymerase II promoter, mitotic 1.2776E-4 14 8 2 6292
    regulation of transcription, mitotic 1.6406E-4 14 9 2 6292
    negative regulation of transcription, mitotic 1.6406E-4 14 9 2 6292
    glycerol metabolic process 2.0481E-4 14 10 2 6292
    alditol metabolic process 2.0481E-4 14 10 2 6292
    small molecule biosynthetic process 3.8937E-4 14 310 5 6292
    tricarboxylic acid cycle 4.7486E-4 14 15 2 6292
    acetyl-CoA catabolic process 4.7486E-4 14 15 2 6292
    cellular metabolic process 5.8003E-4 14 3033 13 6292
    polyol metabolic process 6.8931E-4 14 18 2 6292
    acetyl-CoA metabolic process 8.5383E-4 14 20 2 6292
    coenzyme catabolic process 8.5383E-4 14 20 2 6292
    fermentation 8.5383E-4 14 20 2 6292
    metabolic process 9.4511E-4 14 3157 13 6292
    cofactor catabolic process 1.0354E-3 14 22 2 6292
    cellular respiration 1.1429E-3 14 97 3 6292
    nucleotide-excision repair 3.0877E-3 14 38 2 6292
    small molecule metabolic process 3.877E-3 14 760 6 6292
    succinyl-CoA metabolic process 4.4455E-3 14 2 1 6292
    anaerobic respiration 4.4455E-3 14 2 1 6292
    alcohol biosynthetic process 4.694E-3 14 47 2 6292
    transcription initiation from RNA polymerase II promoter 4.694E-3 14 47 2 6292
    transcription initiation 7.0781E-3 14 58 2 6292
    negative regulation of transcription from RNA polymerase II promoter 7.5599E-3 14 60 2 6292
    citrate metabolic process 8.8726E-3 14 4 1 6292
    cellular biosynthetic process 9.9866E-3 14 1567 8 6292

    Molecular Function Analysis

    Given the number of proteins in the complex (A), total proteins annotated with a given GO term (B), and the total number of annotated proteins (T); the p-value represents the chances of randomly having the number of proteins in the complex annotated with a specific GO term (I).

    Only showing terms with a p-value less than or equal to 0.01.

    GO Term

    P-value

    A

    B

    I

    T

    glycerol-1-phosphatase activity 1.3776E-5 14 3 2 6292
    RNA polymerase II transcription factor activity 2.6553E-3 14 130 3 6292
    transmembrane transporter activity 3.7248E-3 14 306 4 6292
    aconitate hydratase activity 4.4455E-3 14 2 1 6292
    succinate-CoA ligase (ADP-forming) activity 4.4455E-3 14 2 1 6292
    succinate-CoA ligase activity 4.4455E-3 14 2 1 6292
    hydrogen ion transmembrane transporter activity 6.1587E-3 14 54 2 6292
    endopeptidase activity 6.1587E-3 14 54 2 6292
    ATP:ADP antiporter activity 6.6614E-3 14 3 1 6292
    phosphomethylpyrimidine kinase activity 6.6614E-3 14 3 1 6292
    monovalent inorganic cation transmembrane transporter activity 7.3172E-3 14 59 2 6292
    general RNA polymerase II transcription factor activity 8.567E-3 14 64 2 6292
    CoA-ligase activity 8.8726E-3 14 4 1 6292
    acid-thiol ligase activity 8.8726E-3 14 4 1 6292
    hydrolase activity 9.6217E-3 14 911 6 6292

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