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Protein Overview: NIA2_ARATH

General Information:
Name(s) found: NIA2_ARATH [Swiss-Prot]
Description(s) found:
Found 24 descriptions.
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  • Nitrate reductase [NADH] 2 OS=Arabidopsis thaliana GN=NIA2 PE=1 SV=1 [Swiss-Prot]
    • Organism: Arabidopsis thaliana
    Length: 917 amino acids

    Gene Ontology:
    Cellular Component: vacuole [IDA]
    plasma membrane [IDA]
    Biological Process: response to symbiotic fungus [IEP]
    nitric oxide biosynthetic process [IMP]
    response to light stimulus [IMP]
    nitrate assimilation [IMP]
    Molecular Function: nitrate reductase activity [IDA][ISS]
    nitrate reductase (NADH) activity [IDA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAASVDNRQY ARLEPGLNGV VRSYKPPVPG RSDSPKAHQN QTTNQTVFLK PAKVHDDDED  60
   61 VSSEDENETH NSNAVYYKEM IRKSNAELEP SVLDPRDEYT ADSWIERNPS MVRLTGKHPF 120
  121 NSEAPLNRLM HHGFITPVPL HYVRNHGHVP KAQWAEWTVE VTGFVKRPMK FTMDQLVSEF 180
  181 AYREFAATLV CAGNRRKEQN MVKKSKGFNW GSAGVSTSVW RGVPLCDVLR RCGIFSRKGG 240
  241 ALNVCFEGSE DLPGGAGTAG SKYGTSIKKE YAMDPSRDII LAYMQNGEYL TPDHGFPVRI 300
  301 IIPGFIGGRM VKWLKRIIVT TKESDNFYHF KDNRVLPSLV DAELADEEGW WYKPEYIINE 360
  361 LNINSVITTP CHEEILPINA FTTQRPYTLK GYAYSGGGKK VTRVEVTVDG GETWNVCALD 420
  421 HQEKPNKYGK FWCWCFWSLE VEVLDLLSAK EIAVRAWDET LNTQPEKMIW NLMGMMNNCW 480
  481 FRVKTNVCKP HKGEIGIVFE HPTLPGNESG GWMAKERHLE KSADAPPSLK KSVSTPFMNT 540
  541 TAKMYSMSEV KKHNSADSCW IIVHGHIYDC TRFLMDHPGG SDSILINAGT DCTEEFEAIH 600
  601 SDKAKKMLED YRIGELITTG YSSDSSSPNN SVHGSSAVFS LLAPIGEATP VRNLALVNPR 660
  661 AKVPVQLVEK TSISHDVRKF RFALPVEDMV LGLPVGKHIF LCATINDKLC LRAYTPSSTV 720
  721 DVVGYFELVV KIYFGGVHPR FPNGGLMSQY LDSLPIGSTL EIKGPLGHVE YLGKGSFTVH 780
  781 GKPKFADKLA MLAGGTGITP VYQIIQAILK DPEDETEMYV IYANRTEEDI LLREELDGWA 840
  841 EQYPDRLKVW YVVESAKEGW AYSTGFISEA IMREHIPDGL DGSALAMACG PPPMIQFAVQ 900
  901 PNLEKMQYNI KEDFLIF

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..76] deduced N/A No confident structure predictions are available.
    2 View Details [77..537] PSI-BLAST 136.0 crystal structure of the Molybdenum-containing nitrate reducing fragment of Pichia angusta assimilatory nitrate reductase
    3 View Details [538..657] PSI-BLAST 14.522879 Cytochrome b5
    4 View Details [658..917] PSI-BLAST 62.39794 STRUCTURAL STUDIES ON CORN NITRATE REDUCTASE: REFINED STRUCTURE OF THE CYTOCHROME B REDUCTASE FRAGMENT AT 2.5 ANGSTROMS, ITS ADP COMPLEX AND AN ACTIVE SITE MUTANT AND MODELING OF THE CYTOCHROME B DOMAIN

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
    		•  1.94941127592758 bayes_pls_golite062009
  • cytochrome-c oxidase activity
    		•  1.91951393988999 bayes_pls_golite062009
  • oxidoreductase activity, acting on heme group of donors, oxygen as acceptor
    		•  1.91951393988999 bayes_pls_golite062009
  • oxidoreductase activity, acting on heme group of donors
    		•  1.91951393988999 bayes_pls_golite062009
  • sterol desaturase activity
    		•  1.72061589886774 bayes_pls_golite062009
  • C-5 sterol desaturase activity
    		•  1.72061589886774 bayes_pls_golite062009
  • linoleoyl-CoA desaturase activity
    		•  1.72061589886774 bayes_pls_golite062009
  • electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity
    		•  1.68214478072718 bayes_pls_golite062009
  • heme-copper terminal oxidase activity
    		•  1.67979832413891 bayes_pls_golite062009
  • binding
    		•  1.56870596629206 bayes_pls_golite062009
  • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
    		•  1.05227276400356 bayes_pls_golite062009
  • hydrogen ion transmembrane transporter activity
    		•  0.888859895539844 bayes_pls_golite062009
  • oxidoreductase activity
    		•  0.850524633890514 bayes_pls_golite062009
  • oxidoreductase activity, acting on NADH or NADPH
    		•  0.842413203688846 bayes_pls_golite062009
  • monovalent inorganic cation transmembrane transporter activity
    		•  0.808453086600089 bayes_pls_golite062009
  • cation transmembrane transporter activity
    		•  0.602390039366203 bayes_pls_golite062009
  • inorganic cation transmembrane transporter activity
    		•  0.493634303942328 bayes_pls_golite062009
  • protein binding
    		•  0.268795825133297 bayes_pls_golite062009
  • steroid binding
    		•  0.177535173083536 bayes_pls_golite062009
    4
    Term Confidence Notes
  • oxidoreductase activity, acting on NADH or NADPH
    		•  4.30648542212464 bayes_pls_golite062009
  • NADH dehydrogenase (ubiquinone) activity
    		•  4.04090398393497 bayes_pls_golite062009
  • coenzyme binding
    		•  3.33805077160148 bayes_pls_golite062009
  • NADH dehydrogenase activity
    		•  3.28962873390534 bayes_pls_golite062009
  • NADH dehydrogenase (quinone) activity
    		•  3.20430149465423 bayes_pls_golite062009
  • oxidoreductase activity, acting on NADH or NADPH, heme protein as acceptor
    		•  3.111558299994 bayes_pls_golite062009
  • oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor
    		•  3.03192933933799 bayes_pls_golite062009
  • oxidoreductase activity
    		•  3.02740987944465 bayes_pls_golite062009
  • FAD binding
    		•  2.07600122867642 bayes_pls_golite062009
  • cytochrome-b5 reductase activity
    		•  1.5685421124725 bayes_pls_golite062009
  • binding
    		•  1.56341797572673 bayes_pls_golite062009
  • ferredoxin-NADP+ reductase activity
    		•  1.46342400843015 bayes_pls_golite062009
  • ferredoxin reductase activity
    		•  1.46342400843015 bayes_pls_golite062009
  • oxidoreductase activity, acting on iron-sulfur proteins as donors, NAD or NADP as acceptor
    		•  1.46342400843015 bayes_pls_golite062009
  • catalytic activity
    		•  1.45374095556065 bayes_pls_golite062009
  • sulfite reductase (NADPH) activity
    		•  1.43049542627708 bayes_pls_golite062009
  • NADP or NADPH binding
    		•  1.18833847639357 bayes_pls_golite062009
  • demethylase activity
    		•  1.1768217851058 bayes_pls_golite062009
  • oxidoreductase activity, acting on iron-sulfur proteins as donors
    		•  0.73475657802914 bayes_pls_golite062009
  • transferase activity
    		•  0.708491789248912 bayes_pls_golite062009
  • FMN binding
    		•  0.649158247260553 bayes_pls_golite062009
  • riboflavin synthase activity
    		•  0.380537461428523 bayes_pls_golite062009
  • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
    		•  0.33163571415742 bayes_pls_golite062009
  • cofactor binding
    		•  0.205324283520452 bayes_pls_golite062009
  • protein binding
    		•  0.144900113694048 bayes_pls_golite062009
  • oxidoreductase activity, acting on sulfur group of donors
    		•  0.11268023449343 bayes_pls_golite062009


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle