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Protein Overview: rad32

General Information:
Name(s) found: rad32 / SPAC13C5.07 [Sanger Pombe]
Description(s) found:
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  • gi|908896|emb|CAA90458.1| Rad32 nuclease [Schizosaccharomyces pombe] [NCBI NR]
  • pir||S58097 probable dna repair phosphoesterase (EC 3.1.-.-) rad32 - fission yeast (Schizosaccharomyces pombe) [NCBI NR]
  • gi|19113847|ref|NP_592935.1| Rad32 nuclease [Schizosaccharomyces pombe] [NCBI NR]
  • gi|1172822|sp|Q09683.1|RAD32_SCHPO RecName: Full=DNA repair protein rad32 [NCBI NR]
  • Rad32 nuclease [Sanger Pombe]
  • DNA repair protein rad32 OS=Schizosaccharomyces pombe GN=rad32 PE=1 SV=1 [Swiss-Prot]
  • (Q09683) DNA repair protein rad32 [Swiss-Prot]
  • DNA REPAIR PROTEIN; REPAIR EXONUCLEASE; MEIOTIC RECOMBINATION PROTEIN; INVOLVED IN THE PROCESSING OF DNA BREAKS; INVOLVED IN END JOINING; SIMILAR TO S. CEREVISIAE MRE11: Q09683; [pompepVF.fasta]
  • DNA REPAIR PROTEIN; MEIOTIC RECOMBINATION PROTEIN; INVOLVED IN THE PROCESSING OF DNA BREAKS: Q09683; [pompepV13]
  • DNA repair protein rad32 [pompep4896]
  • |rad32||DNA repair protein (PMID 7885834) [pompepnew.fasta]
  • |rad32||exonuclease|Schizosaccharomyces pombe|chr 1|||Manual [pombe041504.fasta]
  • dna repair protein rad32 [Schizosaccharomyces pombe]gi|908896|emb|CAA90458.1| rad32 [Schizosaccharomyces pombe]gi|2130472|pir||S58097 probable dna repair phosphoesterase (EC 3.1.-.-) rad32 - fission yeast (Schizosaccharomyces pombe)gi|1172822|sp|Q09683 [nrdb_11152004_Chlamydomonas]
  • |rad32|mre11|exonuclease|Schizosaccharomyces pombe|chr 1|||Manual [pompep2242005.fasta]
  • |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pombe|chr 1|||Manual [SGD_S-Pombe_na_10-23-2009_con_reversed.fasta]
  • hypothetical protein SPAC13C5.07 [Schizosaccharomyces pombe 972h-] [nr-271106-contam.fasta]
  • SPAC13C5.07 [Schizosaccharomyces pombe] [nr-271106-contam.fasta]
  • DNA repair protein rad32 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=rad32 PE=1 SV=1 [uniprot_sprot.fasta.20160308]
    • Organism: Schizosaccharomyces pombe
    Length: 649 amino acids

    Gene Ontology:
    Cellular Component: Mre11 complex [TASLink to PubMed article]
    Biological Process: DNA damage checkpoint [IMPLink to PubMed article]
    DNA double-strand break processing [IGILink to PubMed article]
    reciprocal meiotic recombination [IMPLink to PubMed article]
    double-strand break repair via homologous recombination [IGILink to PubMed article]
    meiotic gene conversion [IDALink to PubMed article]
    telomere maintenance [IMPLink to PubMed article]
    intra-S DNA damage checkpoint [IMPLink to PubMed article]
    double-strand break repair via nonhomologous end joining [IMPLink to PubMed articleLink to PubMed article]
    cellular protein localization [IGILink to PubMed article]
    meiotic DNA double-strand break formation [TASLink to PubMed article]
    double-strand break repair [IMPLink to PubMed article]
    Molecular Function: protein binding [IPILink to PubMed article]
    double-stranded DNA specific 3'-5' exodeoxyribonuclease activity [TASLink to PubMed article]
    manganese ion binding [IEA]
    single-stranded DNA specific 3'-5' exodeoxyribonuclease activity [TASLink to PubMed article]
    single-stranded DNA specific endodeoxyribonuclease activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPNDPSDMNN ELHNENTIRI LISSDPHVGY GEKDPVRGND SFVSFNEILE IARERDVDMI  60
   61 LLGGDIFHDN KPSRKALYQA LRSLRLNCLG DKPCELELLS DTSLTTGDTA VCNINYLDPN 120
  121 INVAIPVFSI HGNHDDPSGD GRYSALDILQ VTGLVNYFGR VPENDNIVVS PILLQKGFTK 180
  181 LALYGISNVR DERLYHSFRE NKVKFLRPDL YRDEWFNLLT VHQNHSAHTP TSYLPESFIQ 240
  241 DFYDFVLWGH EHECLIDGSY NPTQKFTVVQ PGSTIATSLS PGETAPKHCG ILNITGKDFH 300
  301 LEKIRLRTVR PFIMKDIILS EVSSIPPMVE NKKEVLTYLI SKVEEAITEA NAQWYEAQGT 360
  361 VPVVENEKPP LPLIRLRVDY TGGYQTENPQ RFSNRFVGRV ANATDVVQFY LKKKYTRSKR 420
  421 NDGLYTSAVE DIKINSLRVE SLVNEYLKTN RLECLPEDSL GEAVVNFVEK DDRDAIKECV 480
  481 ETQLNKQINL LVKKRVTEEN LEQEISSIIN DLPKISTTKR KDYEELPEEV SETSINIAEH 540
  541 TPVLKHTSSL LDHHSPLATS SSEHEMEATP SPALLKKTNK RRELPSSLTK KNTRTPQRSK 600
  601 EVKKVPARKL SQSTKKSDKN TQSTLLFYDP SSTTEAQYLD NEDDEILDD

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..350] PSI-BLAST 44.69897 Mre11
    2 View Details [351..474] Pfam 18.267606 No description for PF04152.5 was found. No confident structure predictions are available.
    3 View Details [475..649] MSA 1.061975 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • hydrolase activity
    		•  3.13006743434485 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  2.78838275355792 bayes_pls_golite062009
  • protein serine/threonine phosphatase activity
    		•  1.72108778738758 bayes_pls_golite062009
  • binding
    		•  1.44538582463569 bayes_pls_golite062009
  • catalytic activity
    		•  1.39852585255405 bayes_pls_golite062009
  • phosphoprotein phosphatase activity
    		•  1.23036240099336 bayes_pls_golite062009
  • phosphoric ester hydrolase activity
    		•  0.899406797746366 bayes_pls_golite062009
  • phosphatase activity
    		•  0.76188635342041 bayes_pls_golite062009
  • transporter activity
    		•  0.31687928484091 bayes_pls_golite062009
  • protein binding
    		•  0.283905381578004 bayes_pls_golite062009
  • calcium-dependent protein serine/threonine phosphatase activity
    		•  0.0429959933934001 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Drew K, et al. (2011) The proteome folding project: Proteome-scale prediction of structure and function. Genome Res. 2011 Sep 16


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle