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Protein Overview: THI6

General Information:
Name(s) found: THI6 / YPL214C [SGD]
Description(s) found:
Found 32 descriptions.
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  • Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 540 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed articleLink to PubMed article]
    cytosol [IDALink to PubMed article]
    Biological Process: thiamin biosynthetic process [TASLink to PubMed article]
    Molecular Function: thiamin-phosphate diphosphorylase activity [TASLink to PubMed article]
    hydroxyethylthiazole kinase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVFTKEEVDY SLYLVTDSTM LPPGTTLCSQ VEAGLKNGVT LVQIREKDIE TKNFVAEALE  60
   61 VQKICKKYNV PLIINDRIDV AMAIDADGVH VGQDDMPIPM VRKLLGPSKI LGWSVGKPSE 120
  121 VETLAKWGPD MVDYIGVGTL FPTSTKKNPK KSPMGPQGAI AILDALEEFK ATWCRTVGIG 180
  181 GLHPDNIQRV LCQCVASNGK RSLDGISLVS DIMAAPDACA ATKRLRGLLD ATRYQFVECE 240
  241 LNNTFPTTTS IQNVISQVSN NRPLVQHITN KVHQNFGANV TLALGSSPIM SEIESEVSEL 300
  301 ARIPNASLLL NTGSVAPIEM LKAAINAYNE VNRPITFDPV GYSATETRLC LNNTLLTYGQ 360
  361 FACIKGNCSE ILSLAKLNNH KMKGVDSSSG KTNIDTLVRA TQIVAFQYRT VAVCTGEFDC 420
  421 VADGTFGGEY KLSSGTEGIT AEDLPCVIIE DGPIPIMGDI TASGCSLGST IASFIGGLDS 480
  481 TGKLFDAVVG AVLLYKSAGK LASTRCQGSG SFHVELIDAL YQLFHENKPE KWSASLKKFK 540
  541

    Protein Complex Data

    No complex found for this protein.

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data THI6 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..233] PSI-BLAST 241.228787 Thiamin phosphate synthase
    2 View Details [234..540] PSI-BLAST 266.616428 Hydroxyethylthiazole kinase (THZ kinase, ThiK)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • thiamin-phosphate diphosphorylase activity
    		•  5.90476603396884 bayes_pls_golite062009
  • phosphomethylpyrimidine kinase activity
    		•  1.96653650813022 bayes_pls_golite062009
  • catalytic activity
    		•  1.94308403509017 bayes_pls_golite062009
  • transferase activity
    		•  1.66762065968825 bayes_pls_golite062009
  • transferase activity, transferring alkyl or aryl (other than methyl) groups
    		•  0.73172690404654 bayes_pls_golite062009
  • phosphotransferase activity, phosphate group as acceptor
    		•  0.429889467826441 bayes_pls_golite062009
    2
    Term Confidence Notes
  • phosphomethylpyrimidine kinase activity
    		•  3.972577109569 bayes_pls_golite062009
  • phosphotransferase activity, phosphate group as acceptor
    		•  2.29466565429735 bayes_pls_golite062009
  • kinase activity
    		•  2.28531824242987 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  2.27980720248925 bayes_pls_golite062009
  • transferase activity
    		•  2.15883296431019 bayes_pls_golite062009
  • catalytic activity
    		•  1.74474587246986 bayes_pls_golite062009
  • hydroxyethylthiazole kinase activity
    		•  1.28568044601242 bayes_pls_golite062009
  • thiamin-phosphate diphosphorylase activity
    		•  1.27602595951972 bayes_pls_golite062009
  • carbohydrate kinase activity
    		•  1.12876288824319 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  0.673555832721854 bayes_pls_golite062009
  • pyridoxal kinase activity
    		•  0.217863391052366 bayes_pls_golite062009


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle