Protein Overview: KIN4

General Information:
Name(s) found:	KIN4 / YOR233W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|729900\|sp\|Q01919.1\|KIN4_YEAST RecName: Full=Serine/threonine-protein kinase KIN4 [NCBI NR] gi\|6324807\|ref\|NP_014876.1\| Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; Kin4p [Saccharomyce [NCBI NR] gi\|5515\|emb\|CAA48115.1\| protein kinase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S29344 protein kinase KIN3 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420535\|emb\|CAA99453.1\| KIN4 [Saccharomyces cerevisiae] [NCBI NR] Serine/threonine-protein kinase KIN4 OS=Saccharomyces cerevisiae GN=KIN4 PE=1 SV=1 [Swiss-Prot] KIN4 Ser/thr protein kinase [mips] Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck [SGD] (Q01919) Serine/threonine-protein kinase KIN4 (EC 2.7.1.-) [4932.FASTACCERV.txt] Chr XV from 775846-778248 [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 775845-778247 [yeastorf3.fasta] KIN4, Chr XV from 775845-778247 [yeast_orfs2] KIN4 SGDID:S0005759, Chr XV from 775846-778248, Verified ORF [yeastflysequest.fasta] Kin4p [Saccharomyces cerevisiae]gi\|1420535\|emb\|CAA99453.1\| KIN4 [Saccharomyces cerevisiae]gi\|5515\|emb\|CAA48115.1\| protein kinase [Saccharomyces cerevisiae]gi\|729900\|sp\|Q01919\|KIN4_YEAST Serine/threonine-protein kinase KIN4gi\|322953\|pir\|\|S29344 protein [nrdb_11152004_Chlamydomonas] (Q01919) Serine/threonine-protein kinase KIN4 (EC 2.7.1.37) [cerv4932.FASTAC] KIN4 SGDID:S000005759, Chr XV from 775846-778248, Verified ORF, "Nonessential protein kinase with unknown cellular role" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] KIN4 SGDID:S000005759, Chr XV from 775847-778249, Verified ORF, "Kinase that acts by inhibiting the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud nec [yeast-contam.fasta] KIN4 SGDID:S000005759, Chr XV from 775847-778249, Verified ORF, "Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole b [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Serine/threonine-protein kinase KIN4 [nr-271106-contam.fasta] KIN4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Kinase that acts by inhibiting the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; Kin4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] protein kinase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] KIN4 SGDID:S000005759, Chr XV from 775847-778249, Verified ORF, "Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck" [yeast-bovrabprots.fasta] KIN4 SGDID:S000005759, Chr XV from 775847-778249, Verified ORF, "Kinase that acts by inhibiting the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck" [yeast-contam_custom.fasta] [chemostatdb.fasta] Serine/threonine-protein kinase KIN4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=KIN4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] KIN4 SGDID:S000005759, Chr XV from 775846-778248, Genome Release 64-1-1, Verified ORF, "Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck" [yeast-030211-contam.fasta] KIN4 SGDID:S000005759, Chr XV from 775846-778248, Genome Release 64-2-1, Verified ORF, "Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YOR233W SGDID:S000005759, Chr XV from 775846-778248, Genome Release 64-1-1, Verified ORF, "Serine/threonine protein kinase that inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	800 amino acids
Gene Ontology:
Cellular Component:	cellular bud neck [IDA] cell cortex [IDA] spindle pole body [IDA]
Biological Process:	mitotic cell cycle spindle orientation checkpoint [IGI ][IMP ]
Molecular Function:	protein kinase activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASVPKRHTY GGNVVTDRDR HSLQRNNEIL HPIHKNQRKH ATFGPYIIGS TLGEGEFGKV  60
   61 KLGWTKASSS NEVPKQVAIK LIRRDTIKKD ADKEIKIYRE INALKHLTHP NIIYLEEVLQ 120
  121 NSKYIGIVLE FVSGGEFYKY IQRKRRLKES SACRLFAQLI SGVNYMHYKG LVHRDLKLEN 180
  181 LLLDKHENLV ITDFGFVNEF FEDNELMKTS CGSPCYAAPE LVVSTKAYEA RKADVWSCGV 240
  241 ILYAMLAGYL PWDDDHENPT GDDIARLYKY ITQTPLKFPE YITPIPRDLL RRILVPNPRR 300
  301 RINLQTIKRH VWLKPHEAFL SIQPNYWDEH LQKERPKPPN KGDVGRHSTY SSSASSYSKS 360
  361 RDRNSLIIES TLEQHRMSPQ LATSRPASPT FSTGSKVVLN DTKNDMKESN INGERTSASC 420
  421 RYTRDSKGNG QTQIEQVSAR HSSRGNKHTS VAGLVTIPGS PTTARTRNAP SSKLTEHVKD 480
  481 SSQTSFTQEE FHRIGNYHVP RSRPRPTSYY PGLSRNTADN SLADIPVNKL GSNGRLTDAK 540
  541 DPVPLNAIHD TNKATISNNS IMLLSEGPAA KTSPVDYHYA IGDLNHGDKP ITEVIDKINK 600
  601 DLTHKAAENG FPRESIDPES TSTILVTKEP TNSTDEDHVE SQLENVGHSS NKSDASSDKD 660
  661 SKKIYEKKRF SFMSLYSSLN GSRSTVESRT SKGNAPPVSS RNPSGQSNRS NIKITQQQPR 720
  721 NLSDRVPNPD KKINDNRIRD NAPSYAESEN PGRSVRASVM VSTLREENRS ELSNEGNNVE 780
  781 AQTSTARKVL NFFKRRSMRV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		KIN4 YMR010W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YPP1	KIN4	1	Unpublished Fields Lab Data
View Screen	LSM8	KIN4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	KIN4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..101] [115..131] [344..416]	PSI-BLAST	442.9897	cAMP-dependent PK, catalytic subunit
2	View Details	[102..114] [132..343]	PSI-BLAST	442.9897	cAMP-dependent PK, catalytic subunit
3	View Details	[417..800]	deduced	N/A	No confident structure predictions are available.
4	View Details	[558..664]	deduced	N/A	No confident structure predictions are available.
5	View Details	[665..800]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2