Protein Overview: GYP1

General Information:
Name(s) found:	GYP1 / YOR070C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324644\|ref\|NP_014713.1\| Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion; Gyp1p [Saccharomyces cerevisiae] [NCBI NR] gi\|23821647\|sp\|Q08484.1\|GYP1_YEAST RecName: Full=GTPase-activating protein GYP1; AltName: Full=GAP for YPT1 [NCBI NR] pir\|\|S66953 hypothetical protein YOR070c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|2104882\|emb\|CAA94555.1\| YOR29-21 [Saccharomyces cerevisiae] [NCBI NR] gi\|1420221\|emb\|CAA99263.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] GTPase-activating protein GYP1 OS=Saccharomyces cerevisiae GN=GYP1 PE=1 SV=1 [Swiss-Prot] GYP1 GTPase activating protein for Ypt1p and Sec4p [mips] Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion [SGD] (Q08484) GTPase-activating protein GYP1 (GAP for YPT1) [4932.FASTACCERV.txt] Chr XV from 457821-455908, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 455908-457821, reverse complement [yeastorf3.fasta] GYP1, Chr XV from 455908-457821, reverse complement [yeast_orfs2] GYP1 SGDID:S0005596, Chr XV from 457821-455908, reverse complement, Verified ORF [yeastflysequest.fasta] Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion [Saccharomyces cerevisiae]gi\|2104882\|emb\|CAA94555.1\| YOR29-21 [Saccharomyces c [nrdb_11152004_Chlamydomonas] GYP1 SGDID:S000005596, Chr XV from 457821-455908, reverse complement, Verified ORF, "Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and f [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GYP1 SGDID:S000005596, Chr XV from 457822-455909, reverse complement, Verified ORF, "Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and f [yeast-contam.fasta] Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion; Gyp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YOR29-21 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GTPase-activating protein GYP1 (GAP for YPT1) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GYP1 SGDID:S000005596, Chr XV from 457822-455909, reverse complement, Verified ORF, "Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion" [yeast-bovrabprots.fasta] [chemostatdb.fasta] GTPase-activating protein GYP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GYP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GYP1 SGDID:S000005596, Chr XV from 457820-455907, Genome Release 64-1-1, reverse complement, Verified ORF, "Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion" [yeast-030211-contam.fasta] GYP1 SGDID:S000005596, Chr XV from 457820-455907, Genome Release 64-2-1, reverse complement, Verified ORF, "Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion" [20141208_orf_trans.fasta] YOR070C SGDID:S000005596, Chr XV from 457820-455907, Genome Release 64-1-1, reverse complement, Verified ORF, "Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	637 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] Golgi apparatus [IDA]
Biological Process:	vesicle-mediated transport [IDA]
Molecular Function:	Rab GTPase activator activity [IDA][IMP][IPI][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGVRSAAKEM HERDHNSDSS SLVTSLMKSW RISSASSSKK PSLYKMNTTE STSLPSGYAS  60
   61 SADRDRRTSD GNFEAMAKQQ ASTRRTSNSY SPLRYVNPTL STASNESPRP ALLLRQHHQR 120
  121 HHHHQQPRHS SSGSVGNNCS NSTEPNKKGD RYFKDLDEDW SAVIDDYNMP IPILTNGGFG 180
  181 TPVAPTRTLS RKSTSSSINS ISNMGTSAVR NSSSSFTYPQ LPQLQKEKTN DSKKTQLEIE 240
  241 NERDVQELNS IIQRISKFDN ILKDKTIINQ QDLRQISWNG IPKIHRPVVW KLLIGYLPVN 300
  301 TKRQEGFLQR KRKEYRDSLK HTFSDQHSRD IPTWHQIEID IPRTNPHIPL YQFKSVQNSL 360
  361 QRILYLWAIR HPASGYVQGI NDLVTPFFET FLTEYLPPSQ IDDVEIKDPS TYMVDEQITD 420
  421 LEADTFWCLT KLLEQITDNY IHGQPGILRQ VKNLSQLVKR IDADLYNHFQ NEHVEFIQFA 480
  481 FRWMNCLLMR EFQMGTVIRM WDTYLSETSQ EVTSSYSMSS NDIKPPVTPT EPRVASFVTP 540
  541 TKDFQSPTTA LSNMTPNNAV EDSGKMRQSS LNEFHVFVCA AFLIKWSDQL MEMDFQETIT 600
  601 FLQNPPTKDW TETDIEMLLS EAFIWQSLYK DATSHWL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Qiu et al. (2008)		GYP1 GYP5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	PAN1	No Comments	Toshima J, et al (2006)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CIN4	GYP1	2	Uetz P, et al. (2000)
View Screen	UBP6	GYP1	1	Unpublished Fields Lab Data
View Screen	YJR012C	GYP1	1	Hazbun TR, et al. (2003)
View Screen	PRR1	GYP1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	GYP1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..238]	MSA	6.149997	View MSA. No confident structure predictions are available.
2	View Details	[239..445] [499..539]	PSI-BLAST	10084.0	Ypt/Rab-GAP domain of gyp1p
3	View Details	[446..498] [540..637]	PSI-BLAST	10084.0	Ypt/Rab-GAP domain of gyp1p

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
GTPase regulator activity	1.01713431388195	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.01642360334206	bayes_pls_golite062009
GTPase activator activity	0.505325037853263	bayes_pls_golite062009
enzyme regulator activity	0.434818760059511	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2