Protein Overview: SMC5

General Information:
Name(s) found:	SMC5 / YOL034W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74627239\|sp\|Q08204.1\|SMC5_YEAST RecName: Full=Structural maintenance of chromosomes protein 5 [NCBI NR] gi\|6324539\|ref\|NP_014608.1\| Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair [NCBI NR] pir\|\|S66717 hypothetical protein YOL034w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1419827\|emb\|CAA99034.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Structural maintenance of chromosomes protein 5 OS=Saccharomyces cerevisiae GN=SMC5 PE=1 SV=1 [Swiss-Prot] SMC5 Structural maintenance of chromosomes (SMC) protein, required for cell viability [mips] Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair [SGD] >Q08204 (Q08204) Chromosome XV reading frame ORF YOL034W [4932.FASTACCERV.txt] Chr XV from 259923-263204 [hmr_yeast5_DM3-1_pombe.fasta] YOL034W, Chr XV from 259923-263204 [yeast_orfs2] SMC5 SGDID:S0005394, Chr XV from 259923-263204, Verified ORF [yeastflysequest.fasta] Smc5p [Saccharomyces cerevisiae]gi\|1419827\|emb\|CAA99034.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2131997\|pir\|\|S66717 hypothetical protein YOL034w - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] (Q08204) S.cerevisiae chromosome XV reading frame ORF YOL034w [cerv4932.FASTAC] SMC5 SGDID:S000005394, Chr XV from 259923-263204, Verified ORF, "Structural maintenance of chromosomes (SMC) protein, interacts with Smc6p and Nse1p to form a large complex; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SMC5 SGDID:S000005394, Chr XV from 259924-263205, Verified ORF, "Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe [yeast-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Structural maintenance of chromosome 5 [nr-271106-contam.fasta] Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair; Smc5p [Saccharomyces cerev [nr-271106-contam.fasta] SMC5 SGDID:S000005394, Chr XV from 259924-263205, Verified ORF, "Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Structural maintenance of chromosomes protein 5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SMC5 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SMC5 SGDID:S000005394, Chr XV from 259923-263204, Genome Release 64-1-1, Verified ORF, "Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair" [yeast-030211-contam.fasta] SMC5 SGDID:S000005394, Chr XV from 259923-263204, Genome Release 64-2-1, Verified ORF, "Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair" [20141208_orf_trans.fasta] YOL034W SGDID:S000005394, Chr XV from 259923-263204, Genome Release 64-1-1, Verified ORF, "Structural maintenance of chromosomes (SMC) protein; essential subunit of the Mms21-Smc5-Smc6 complex; required for growth and DNA repair; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1093 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] Smc5-Smc6 complex [IPI ]
Biological Process:	cell proliferation [IDA] DNA repair [IDA][IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTSLIDLGRY VERTHHGEDT EPRSKRVKIA KPDLSSFQPG SIIKIRLQDF VTYTLTEFNL  60
   61 SPSLNMIIGP NGSGKSTFVC AVCLGLAGKP EYIGRSKKVE DFIKNGQDVS KIEITLKNSP 120
  121 NVTDIEYIDA RDETIKITRI ITRSKRRSDY LINDYQVSES VVKTLVAQLN IQLDNLCQFL 180
  181 SQERVEEFAR LKSVKLLVET IRSIDASLLD VLDELRELQG NEQSLQKDLD FKKAKIVHLR 240
  241 QESDKLRKSV ESLRDFQNKK GEIELHSQLL PYVKVKDHKE KLNIYKEEYE RAKANLRAIL 300
  301 KDKKPFANTK KTLENQVEEL TEKCSLKTDE FLKAKEKINE IFEKLNTIRD EVIKKKNQNE 360
  361 YYRGRTKKLQ ATIISTKEDF LRSQEILAQT HLPEKSVFED IDIKRKEIIN KEGEIRDLIS 420
  421 EIDAKANAIN HEMRSIQRQA ESKTKSLTTT DKIGILNQDQ DLKEVRDAVL MVREHPEMKD 480
  481 KILEPPIMTV SAINAQFAAY LAQCVDYNTS KALTVVDSDS YKLFANPILD KFKVNLRELS 540
  541 SADTTPPVPA ETVRDLGFEG YLSDFITGDK RVMKMLCQTS KIHTIPVSRR ELTPAQIKKL 600
  601 ITPRPNGKIL FKRIIHGNRL VDIKQSAYGS KQVFPTDVSI KQTNFYQGSI MSNEQKIRIE 660
  661 NEIINLKNEY NDRKSTLDAL SNQKSGYRHE LSELASKNDD INREAHQLNE IRKKYTMRKS 720
  721 TIETLREKLD QLKREARKDV SQKIKDIDDQ IQQLLLKQRH LLSKMASSMK SLKNCQKELI 780
  781 STQILQFEAQ NMDVSMNDVI GFFNEREADL KSQYEDKKKF VKEMRDTPEF QSWMREIRSY 840
  841 DQDTKEKLNK VAEKYEEEGN FNLSFVQDVL DKLESEIAMV NHDESAVTIL DQVTAELREL 900
  901 EHTVPQQSKD LETIKAKLKE DHAVLEPKLD DIVSKISARF ARLFNNVGSA GAVRLEKPKD 960
  961 YAEWKIEIMV KFRDNAPLKK LDSHTQSGGE RAVSTVLYMI ALQEFTSAPF RVVDEINQGM1020
 1021 DSRNERIVHK AMVENACAEN TSQYFLITPK LLTGLHYHEK MRIHCVMAGS WIPNPSEDPK1080
 1081 MIHFGETSNY SFD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Hazbun TR, et al. (2003)		KRE29 MMS21 NSE1 NSE3 NSE4 NSE5 SMC5 SMC6
View Details	Krogan NJ, et al. (2006)		GDH2 MMS21 NSE3 NSE4 SMC5 YOR352W
View Details	Qiu et al. (2008)		NSE1 SMC5 SMC6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NSE5	No Comments	Hazbun TR, et al. (2003)
View Run	SMC5	No Comments	Hazbun TR, et al. (2003)
View Run	NSE3	No Comments	Hazbun TR, et al. (2003)
View Run	NSE5	No Comments	Hazbun TR, et al. (2003)
View Run	NSE3	No Comments	Hazbun TR, et al. (2003)
View Run	SMC5	No Comments	Hazbun TR, et al. (2003)
View Run	NSE5	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SMC5	FRT2	2	Hazbun TR, et al. (2003)
View Screen	SMC5	SAP1	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	SMC5 and SPC42	Unpublished Data
View Data	SMC5	Unpublished Data
View Data	SMC5 and SPC42	Unpublished Data
View Data	SMC5	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..126]	PSI-BLAST	8.0	Rad50
2	View Details	[127..283]	PSI-BLAST	23.69897	Heavy meromyosin subfragment
3	View Details	[284..300] [372..404]	PSI-BLAST	2.154902	Colicin Ia; Colicin Ia, N-terminal domain
4	View Details	[301..371] [405..435]	PSI-BLAST	2.154902	Colicin Ia; Colicin Ia, N-terminal domain
5	View Details	[436..713]	PSI-BLAST	2.154902	Colicin Ia; Colicin Ia, N-terminal domain
6	View Details	[714..903]	PSI-BLAST	8.522879	Tropomyosin
7	View Details	[904..1093]	PSI-BLAST	25.522879	Smc head domain

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2